MemPro™ Membrane Protein Families

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Creative Biostructure provides one-stop MemPro™ gene-to-structure services for membrane protein production , purification , crystallization , structure determination and characterization . Scientists from Creative Biostructure have rich experience and advanced techniques (e.g., energy-dispersive X-ray spectroscopy , NMR spectroscopy and Cyro-Electron Microscopy ) to serve customers with membrane protein structure determination and analysis .

Over 50% of modern medical drug targets at membrane proteins, whose structures and functions are largely unknown. However, membrane proteins are known to be difficult to crystallize with traditional methods because of the rich hydrophobic and insoluble features. Creative Biostructure takes advantage of the MemPro™ cell-free system and high-throughput screening detergents for the optimization of high-yield production and purification strategies for membrane proteins in natural conformations.

Creative Biostructure guarantees the following high-standard services:

√ Harvest of purified and functional membrane proteins with proper documentation;

√ Structural and functional analysis of membrane proteins;

√ Building of structure models for Computer-Aided Drug Design (CADD) and ligand design.

Creative Biostructure can provide custom MemPro™ protein production and analysis services for membrane proteins including but not limited to:

Family name	Description
GPCR	7-transmembrane domain receptors transducing extracellular signals into cells.
Ion channels	Important transmembrane (TM) pore-forming proteins
Transporters	Membrane transports play crucial roles in all biological processes.
Rhodopsin-like receptors and pumps	The most famous GPCRs
ABC transporters	Using ATP to translocate various substrates across membranes
Major Intrinsic Protein (MIP)/FNT superfamily	Specific for the transport of water and small neutral solutes
Pentameric ligand-gated ion channels	Ligand-dependent signal conversion from chemical signals to electric signals.
Ammonia and urea transporters	Regulating transepithelial ammonia secretion
Major Facilitator Superfamily (MFS)	Facilitating small solute movement in response to chemical concentration gradients of sugars, drugs, etc .
Sulfatase	Hydrolysis of sulfate esters; involved in sulfur cycling
Peptidase AD	Cleaving amyloid precursor; its dysfunction will cause Alzheimer's disease.
MAPEG	Eicosanoid and Glutathione metabolism proteins
Transmembrane proteins 14	Heme biosynthesis
Hypoxia induced protein	Catalyzing the reduction of oxygen; assemble of respiratory
Peptidase family M48	Proteolytical removal of farnesylated C-terminal
Fatty acid desaturase/hydroxylase	Biosynthesis of membrane phospholipids, cholesterol ester and triglycerides
Receptor-type kinases	Tyrosine-protein kinases, regulate central nervous system, gene transcription and cell differentiation.
T-cell surface glycoprotein CD3 zeta chain	Expression of TCR complex.
Immunoglobulin superfamily	Recognition, binding and adhesion process of cells.
Integrin	Bridges for cell-cell and cell-extracellular matrix interaction
Toll-like receptors	Innate immune system
Protein jagged	Notch signaling; embryonic development
TYROBP	Containing ITAM; active signal transduction associated with TCR
Stannin	Initiating the apoptotic cascade
Glycophorin	Glycoprotein of the membrane of red blood cell carrying sugar molecules.
SNARE complex	Vesicle fusion
BNip3 protein family	Protect cell from apoptosis
Bcl-2	Regulating cell-death, either induce apoptotic or inhibit apoptosis.
Single-helix ATPase regulators	ATPase regulators; P-P-bond hydrolysis-driven transporter
Voltage-gated K+ channel accessory protein (MinK)	Returning the depolarized cell to rest state.
Cytochrome P450	Terminal oxidase enzyme in electron transfer chain.
Transmembrane proteins with NAD(P)-binding Rossmann-fold domains	Monoamine oxidase; deaminates norepinephrine, epinephrine, serotonin and dopamine.
Oligosaccaryltransferase	Transferring a 14-sugar oligosaccharide from dolichol to nascent protein
N-(deoxy)ribosyltransferase-like superfamily	CD38; synthesis and hydrolysis of cyclic ADP-ribose
Peptidase MA	Zinc metalloenzymes; Cleave amino acids from the N-terminal
TPR alpha-helical repeat proteins	Containing anaphase-promoting complex; mediating protein-protein interaction.
Acyl CoA binding protein	Binding with medium/long-chain acyl-CoA esters and functioning as an intracellular carrier of acyl-CoA esters
Amyloid beta A4 protein	Involved in Alzheimer's diseases
Lysosome-associated membrane glycoprotein	Specific to lysosomes; CD107
Tumor necrosis factor receptor	Binding with TNF and NGF; interacting with a variety of signal molecules. Highly associated with apoptosis.
Type I phosphodiesterase/nucleotide pyrophosphatase	Hydrolyzing nucleoside 5' triphosphates to their monophosphates and di-adenosine polyphosphates.
Squalene/phytoene synthase	Cholesterol biosynthetic pathway
FMN-linked oxidoreductases	DHODH; biosynthesis of orotate
Neutral/alkaline ceramidase	Converting sphingolipid to sphingosine
Aldehyde dehydrogenase	ALDH; Oxidation of aldehydes
Mitochondrial and plastid porins	Calcium ion transporter in mitochondrial. Regulate cell metabolism and apoptosis
HSD-11B	Converting cortisone to cortisol
stearoyl -CoA desaturase 1	A δ-9 fatty acid desaturase that catalyzes the synthesis of monounsaturated fatty acids.
CAAX protease	Integral membrane protease located at the endoplasmic reticulum to catalyze proteins contain a CAAX motifs.
EPHA1	A receptor tyrosine kinase that binds membrane-associated ephrin-A family ligands.
EPHA2	A receptor tyrosine kinase that binds membrane-associated ephrin-A family ligands.
EPHA3	A receptor tyrosine kinase that binds membrane-associated ephrin-A family ligands.
EPHA4	A receptor tyrosine kinase that binds membrane-associated ephrin-A family ligands.
HER2/ErbB2	A transmembrane glycoprotein receptor tyrosine kinase involved in the signal transduction pathways.
HER3/ErbB3	A member of the epidermal growth factor receptor (EGFR/ERBB) family of receptor tyrosine kinases; can form active heterodimers with HER2/ErbB2.
HER4/ErbB4	A ubiquitously expressed member of the epidermal growth factor (EGF/ErbB) family of receptor tyrosine kinases.
5-lipoxygenase–activating protein	A protein that in humans is encoded by the ALOX5AP gene; necessary for the activation of 5-lipoxygenase and the production of leukotrienes.
Gamma secretase	A multi-subunit protease complex that cleaves single-pass transmembrane proteins.
Glucose transporter	A group of membrane proteins that facilitate the transport of glucose over a plasma membrane.
Insulin receptor	A transmembrane receptor that is activated by insulin, IGF-I, IGF-II and belongs to the group of tyrosine kinase receptors.
LRR-containing membrane proteins	A structural motif of 20-30 amino acid stretches that are abundant in the hydrophobic leucine residue.
Leukotriene C4 synthase	An enzyme that in humans is encoded by the LTC4S gene; converts leukotriene A4 and glutathione to leukotriene C4.
Microsomal prostaglandin E synthase	A member of membrane associated proteins in eicosanoid and glutathione metabolism (MAPE) superfamily that catalyze the biosynthesis of active PGE2 from cyclooxygenases (COX-1- and COX-2) derived PGH2.
VEGF receptors	A class of receptors for vascular endothelial growth factor (VEGF); can be classified as membrane bound VEGFR (mbVEGFR) and soluble VEGFR (sVEGFR).
Chemokine Receptor 8 Protein	Significantly highly expressed in various tumors and inflammation sites