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List of Restriction Enzyme Sites

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Restriction Enzyme Name Search

Browse restriction enzyme based on alphabetical listing:

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Enzyme NamePrototypeSequenceCut SiteOverhang
AaaIXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
AagIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
AarICACCTGCCACCTGCNNNN^NNNN
GTGGACGNNNNNNNN^
5' - NNNN
AasIDrdIGACNNNNNNGTCGACNNNN^NNGTC
CTGNN^NNNNCAG
NN - 3'
AatIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
AatIIGACGTCGACGT^C
C^TGCAG
ACGT - 3'
AauIBsp1407ITGTACAT^GTACA
ACATG^T
5' - CATG
AbaIBclITGATCAT^GATCA
ACTAG^T
5' - CTAG
AbeIBbvCICCTCAGCCC^TCAGC
GGAGT^CG
5' - AGT
AbrIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
AccIGTMKACGT^MKAC
CAMK^TG
5' - MK
AccIIFnuDIICGCGCG^CG
GC^GC
Blunt
AccIIIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Acc16IMstITGCGCATGC^GCA
ACG^CGT
Blunt
Acc36IBspMIACCTGCACCTGCNNNN^NNNN
TGGACGNNNNNNNN^
5' - NNNN
Acc65IKpnIGGTACCG^GTACC
CCATG^G
5' - CATG
Acc113IScaIAGTACTAGT^ACT
TCA^TGA
Blunt
AccB1IHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
AccB2IHaeIIRGCGCYRGCGC^Y
R^CGCGY
GCGC - 3'
AccB7IPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
AccBSIBsrBICCGCTCCCG^CTC
GGC^GAG
Blunt
AccEBIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
AceITseIGCWGCG^CWGC
CGWC^G
5' - GWC
AceIINheIGCTAGCGCTAG^C
C^GATCG
CTAG - 3'
AceIIICAGCTCCAGCTCNNNNNNN^NNNN
GTCGAGNNNNNNNNNNN^
5' - NNNN
AciICCGCC^CGC
GGC^G
5' - GC
AclIAACGTTAA^CGTT
TTGC^AA
5' - GC
AclNISpeIACTAGTA^CTAGT
TGATC^A
5' - GATC
AclWIBinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
AcpIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
AcpIIPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
AcrIIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
AcsIApoIRAATTYR^AATTY
RTTAA^Y
5' - TTAA
AcuIEco57ICTGAAGCTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN
NN - 3'
AcvIPmaCICACGTGCAC^GTG
GTG^CAC
Blunt
AcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
AdeIDraIIICACNNNGTGCACNNN^GTG
GTG^NNNCAC
NNN - 3'
AeuIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
AfaIRsaIGTACGT^AC
CA^TG
Blunt
Afa22MIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
Afa16RIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
AfeIEco47IIIAGCGCTAGC^GCT
TCG^CGA
Blunt
AflIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
AflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
AflIIIACRYGTA^CRYGT
TGRYC^A
5' - GRYC
AgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
AglIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
AhaICauIICCSGGCC^SGG
GGS^CC
5' - S
AhaIIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
AhaIIITTTAAATTT^AAA
AAA^TTT
Blunt
AhaB8IKpnIGGTACCG^GTACC
CCATG^G
5' - CATG
AhdIEam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
AhlISpeIACTAGTA^CTAGT
TGATC^A
5' - GATC
AhyISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
AitIEco47IIIAGCGCTAGC^GCT
TCG^CGA
Blunt
AjnIEcoRIICCWGG^CCWGG
GGWCC^
5' - GGWCC
AjoIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
AleIOliICACNNNNGTGCACNN^NNGTG
GTGNN^NNCAC
Blunt
AlfIGCANNNNNNTGCGCANNNNNNTGCNNNNNNNNNNNN^
CGTNNNNNNACGNNNNNNNNNN^NN
NN - 3'
AliIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
AliAJIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
AloIGAACNNNNNNTCCGAACNNNNNNTCCNNNNNNNNNNNN^
CTTGNNNNNNAGGNNNNNNN^NNNNN
NNNNN - 3'
AluIAGCTAG^CT
TC^GA
Blunt
AlwIBinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
Alw21IHgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
Alw26IBsmAIGTCTCGTCTCN^NNNN
CAGAGNNNNN^
5' - NNNN
Alw44IApaLIGTGCACG^TGCAC
CACGT^G
5' - ACGT
AlwNICAGNNNCTGCAGNNN^CTG
GTC^NNNGAC
NNN - 3'
AlwXIBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
Ama87IAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
AocISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
AocIISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
AorIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Aor13HIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Aor51HIEco47IIIAGCGCTAGC^GCT
TCG^CGA
Blunt
AosIMstITGCGCATGC^GCA
ACG^CGT
Blunt
AosIIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
ApaIGGGCCCGGGCC^C
C^CCGGG
GGCC - 3'
ApaBIGCANNNNNTGCGCANNNNN^TGC
CGT^NNNNNACG
NNNNN - 3'
ApaCIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
ApaLIGTGCACG^TGCAC
CACGT^G
5' - ACGT
ApaORIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
ApeKITseIGCWGCG^CWGC
CGWC^G
5' - GWC
ApiIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
ApoIRAATTYR^AATTY
RTTAA^Y
5' - TTAA
ApyIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
AquIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
AscIGGCGCGCCGG^CGCGCC
CCGCGC^GG
5' - GCGC
AseIVspIATTAATAT^TAAT
TAAT^TA
5' - AT
AseIICauIICCSGGCC^SGG
GGS^CC
5' - S
AsiIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
AsiAIAgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
AsiSISgfIGCGATCGCGCGAT^CGC
CGC^TAGCG
AT - 3'
AsnIVspIATTAATAT^TAAT
TAAT^TA
5' - AT
AspITth111IGACNNNGTCGACN^NNGTC
CTGNN^NCAG
5' - N
Asp700IXmnIGAANNNNTTCGAANN^NNTTC
CTTNN^NNAAG
Blunt
Asp713IPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Asp718IKpnIGGTACCG^GTACC
CCATG^G
5' - CATG
Asp745IAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
AspAIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
AspA2IAvrIICCTAGGC^CTAGG
GGATC^C
5' - GATC
AspEIEam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
AspHIHgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
Asp10HIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Asp10HIIPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
Asp26HIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
Asp27HIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
Asp35HIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
Asp36HIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
Asp40HIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
Asp50HIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
AspLEIHhaIGCGCGCG^C
C^GCG
CG - 3'
AspMIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
AspMDIMboIGATC^GATC
CTAG^
5' - CTAG
AspNINlaIVGGNNCCGGN^NCC
CCN^NGG
Blunt
AspS9IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
AssIScaIAGTACTAGT^ACT
TCA^TGA
Blunt
AstWIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
AsuIGGNCCG^GNCC
CCNG^G
5' - CNG
AsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
AsuIIIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
AsuC2ICauIICCSGGCC^SGG
GGS^CC
5' - S
AsuHPIHphIGGTGAGGTGANNNNNNNN^
CCACTNNNNNNN^N
N - 3'
AsuNHINheIGCTAGCG^CTAGC
CGATC^G
5' - GATC
AtsITth111IGACNNNGTCGACN^NNGTC
CTGNN^NCAG
5' - N
AvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
AvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
AvcIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
AviIIMstITGCGCATGC^GCA
ACG^CGT
Blunt
AvrIICCTAGGC^CTAGG
GGATC^C
5' - GATC
AvrBIIAvrIICCTAGGC^CTAGG
GGATC^C
5' - GATC
AxyISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
AanIPsiITTATAATTA^TAA
AAT^ATT
Blunt
AbsICCTCGAGGCC^TCGAGG
GGAGCT^CC
5' - AGCT
AcoICfrIYGGCCRY^GGCCR
YCCGG^R
5' - CCGG
AfiIGCNNNNNNNGGGCNNNNN^NNGG
CGNN^NNNNNCC
NNN - 3'
AjiIBtrICACGTCCAC^GTC
GTG^CAG
Blunt
AluBIAluIAGCTAG^CT
TC^GA
Blunt
AsiGIAgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
Bac36IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BaeIACNNNNGTAYCACNNNNGTAYCNNNNNNNNNNNN^
TGNNNNCATYGNNNNNNN^NNNNN
NNNNN - 3'
BalITGGCCATGG^CCA
ACC^GGT
Blunt
Bal228IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
BamNxIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
BanIHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
BanIIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
BanIIIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BanAIHaeIIIGGCCGG^CC
CC^GG
Blunt
BasIPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
BauIBsiICACGAGC^ACGAG
GTGCT^C
5' - TGCT
BavIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
BavAIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
BavAIIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BavBIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
BavBIIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BavCIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BbeINarIGGCGCCGGCGC^C
C^CGCGG
GCGC - 3'
BbiIIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
Bbi24IMluIACGCGTA^CGCGT
TGCGC^A
5' - GCGC
BbrIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
Bbr7IBbvIIGAAGACGAAGACNNNNNNN^NNNN
CTTCTGNNNNNNNNNNN^
5' - NNNN
BbrPIPmaCICACGTGCAC^GTG
GTG^CAC
Blunt
BbsIBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BbuISphIGCATGCGCATG^C
C^GTACG
CATG - 3'
BbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
BbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
Bbv12IHgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
Bbv16IIBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BbvAIXmnIGAANNNNTTCGAANN^NNTTC
CTTNN^NNAAG
Blunt
BbvAIIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BbvAIIIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
BbvBIHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
BbvCICCTCAGCCC^TCAGC
GGAGT^CG
5' - AGT
Bca77IBetIWCCGGWW^CCGGW
WGGCC^W
5' - GGCC
BccICCATCCCATCNNNN^N
GGTAGNNNNN^
5' - N
Bce4IMwoIGCNNNNNNNGCGCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
Bce22IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Bce83ICTTGAGCTTGAGNNNNNNNNNNNNNNNN^
GAACTCNNNNNNNNNNNNNN^NN
NN - 3'
Bce243IMboIGATC^GATC
CTAG^
5' - CTAG
Bce751IBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
BceAIBcefIACGGCACGGCNNNNNNNNNNNN^NN
TGCCGNNNNNNNNNNNNNN^
5' - NN
BceBIFnuDIICGCGCG^CG
GC^GC
Blunt
BceCIMwoIGCNNNNNNNGCGCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
BcefIACGGCACGGCNNNNNNNNNNNN^N
TGCCGNNNNNNNNNNNNN^
5' - N
BcgICGANNNNNNTGCCGANNNNNNTGCNNNNNNNNNNNN^
GCTNNNNNNACGNNNNNNNNNN^NN
NN - 3'
Bci29IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BciBIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BciBIIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BciVIGTATCCGTATCCNNNNNN^
CATAGGNNNNN^N
N - 3'
BclITGATCAT^GATCA
ACTAG^T
5' - CTAG
BcmIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BcnICauIICCSGGCC^SGG
GGS^CC
5' - S
BcoIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
Bco5IKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
Bco27IHpaIICCGGC^CGG
GGC^C
5' - GC
Bco116IKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
Bco118ICfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
BcoAIPmaCICACGTGCAC^GTG
GTG^CAC
Blunt
BcoKIKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
BcuISpeIACTAGTA^CTAGT
TGATC^A
5' - GATC
BcuAIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
BdiIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BdiSISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
BecAIIHaeIIIGGCCGG^CC
CC^GG
Blunt
BepIFnuDIICGCGCG^CG
GC^GC
Blunt
BetIWCCGGWW^CCGGW
WGGCC^W
5' - GGCC
BfaIMaeICTAGC^TAG
GAT^C
5' - AT
BfiIACTGGGACTGGGNNNNN^
TGACCCNNNN^N
N - 3'
Bfi57IMboIGATC^GATC
CTAG^
5' - CTAG
Bfi89ICfrIYGGCCRY^GGCCR
YCCGG^R
5' - CCGG
BflIBsiYICCNNNNNNNGGCCNNNNN^NNGG
GGNN^NNNNNCC
NNN - 3'
BfmISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
BfrIAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
BfrBIAvaIIIATGCATATG^CAT
TAC^GTA
Blunt
BfuIBciVIGTATCCGTATCCNNNNNN^
CATAGGNNNNN^N
N - 3'
BfuAIBspMIACCTGCACCTGCNNNN^NNNN
TGGACGNNNNNNNN^
5' - NNNN
BfuCIMboIGATC^GATC
CTAG^
5' - CTAG
BglIGCCNNNNNGGCGCCNNNN^NGGC
CGGN^NNNNCCG
NNN - 3'
BglIIAGATCTA^GATCT
TCTAG^A
5' - CTAG
BimIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Bim19IAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Bim19IIHaeIIIGGCCGG^CC
CC^GG
Blunt
BinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
BlfIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Bli41IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
Bli86IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
Bli736IEco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
BliAIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BliHKISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
BliRIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BlnIAvrIICCTAGGC^CTAGG
GGATC^C
5' - GATC
BloHIXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
BloHIIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
BlpIEspIGCTNAGCGC^TNAGC
CGANT^CG
5' - ANT
BluIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
Bme12IMboIGATC^GATC
CTAG^
5' - CTAG
Bme18IAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
Bme142IHaeIIRGCGCYRGC^GCY
RCG^CGY
Blunt
Bme216IAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
Bme361IHaeIIIGGCCGG^CC
CC^GG
Blunt
Bme585IFauICCCGCCCCGCNNNN^NN
GGGCGNNNNNN^
5' - NN
Bme1390IScrFICCNGGCC^NGG
GGN^CC
5' - N
Bme1580IBseSIGKGCMCGKGCM^C
C^KCGMG
KGCM - 3'
BmgBIBtrICACGTCCAC^GTC
GTG^CAG
Blunt
BmrIBfiIACTGGGACTGGGNNNNN^
TGACCCNNNN^N
N - 3'
BmtINheIGCTAGCGCTAG^C
C^GATCG
CTAG - 3'
BmyISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
BnaIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
BoxIPshAIGACNNNNGTCGACNN^NNGTC
CTGNN^NNCAG
Blunt
BpcISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
BpiIBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BplIGAGNNNNNCTCGAGNNNNNCTCNNNNNNNNNNNNN^
CTCNNNNNGAGNNNNNNNN^NNNNN
NNNNN - 3'
BpmIGsuICTGGAGCTGGAGNNNNNNNNNNNNNNNN^
GACCTCNNNNNNNNNNNNNN^NN
NN - 3'
BpoAIVspIATTAATAT^TAAT
TAAT^TA
5' - AT
BptIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BpuIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
Bpu10ICCTNAGCCC^TNAGC
GGANT^CG
5' - ANT
Bpu14IAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Bpu95IFnuDIICGCGCG^CG
GC^GC
Blunt
Bpu1102IEspIGCTNAGCGC^TNAGC
CGANT^CG
5' - ANT
BpuAIBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BpuAmISacIGAGCTCGAG^CTC
CTC^GAG
Blunt
BpuB5ISplICGTACGC^GTACG
GCATG^C
5' - CATG
BpuDIBpu10ICCTNAGCCC^TNAGC
GGANT^CG
5' - ANT
BpuEIBce83ICTTGAGCTTGAGNNNNNNNNNNNNNNNN^
GAACTCNNNNNNNNNNNNNN^NN
NN - 3'
BpuSIFinIGGGACGGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^
5' - NNNN
BsaIEco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
Bsa29IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BsaAIYACGTRYAC^GTR
YTG^CAR
Blunt
BsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
BsaHIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
BsaJISecICCNNGGC^CNNGG
GGNNC^C
5' - GNNC
BsaMIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
BsaOIMcrICGRYCGCGRY^CG
GC^RYGC
RY - 3'
BsaWIBetIWCCGGWW^CCGGW
WGGCC^W
5' - GGCC
BsaXIACNNNNNCTCCACNNNNNCTCCNNNNNNNNNN^
TGNNNNNGAGGNNNNNNN^NNN
NNN - 3'
BscIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
Bsc4IBsiYICCNNNNNNNGGCCNNNNN^NNGG
GGNN^NNNNNCC
NNN - 3'
Bsc91IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
Bsc107IBsiYICCNNNNNNNGGCCNNNNN^NNGG
GGNN^NNNNNCC
NNN - 3'
BscAISfaNIGCATCGCATCNNNN^NN
CGTAGNNNNNN^
5' - NN
BscBINlaIVGGNNCCGGN^NCC
CCN^NGG
Blunt
BscCIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
BscFIMboIGATC^GATC
CTAG^
5' - CTAG
Bse1IBsrIACTGGACTGGN^
TGAC^CN
GN - 3'
Bse8IBsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
Bse15IAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
Bse16IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bse17IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bse21ISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
Bse24IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bse64IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
Bse118ICfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
Bse634ICfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
BseAIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
BseBIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BseCIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BseDISecICCNNGGC^CNNGG
GGNNC^C
5' - GNNC
Bse3DIBsrDIGCAATGGCAATGNN^
CGTTAC^NN
NN - 3'
BseGIFokIGGATGGGATGNN^
CCTAC^NN
NN - 3'
BseJIBsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
BseKIBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
BseLIBsiYICCNNNNNNNGGCCNNNNN^NNGG
GGNN^NNNNNCC
NNN - 3'
BseMIBsrDIGCAATGGCAATGNN^
CGTTAC^NN
NN - 3'
BseMIICTCAGCTCAGNNNNNNNNNN^
GAGTCNNNNNNNN^NN
NN - 3'
BseNIBsrIACTGGACTGGN^
TGAC^CN
GN - 3'
BsePIGCGCGCG^CGCGC
CGCGC^G
5' - GCGC
BseQIHaeIIIGGCCGG^CC
CC^GG
Blunt
BseRIGAGGAGGAGGAGNNNNNNNNNN^
CTCCTCNNNNNNNN^NN
NN - 3'
BseSIGKGCMCGKGCM^C
C^KCGMG
KGCM - 3'
BseT9IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
BseT10IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
BseXIBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
BseX3IXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
BseYICCCAGCC^CCAGC
GGGTC^G
5' - GGTC
BseZIKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
BsgIGTGCAGGTGCAGNNNNNNNNNNNNNNNN^
CACGTCNNNNNNNNNNNNNN^NN
NN - 3'
BshIHaeIIIGGCCGG^CC
CC^GG
Blunt
Bsh45IHgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
Bsh1236IFnuDIICGCGCG^CG
GC^GC
Blunt
Bsh1285IMcrICGRYCGCGRY^CG
GC^RYGC
RY - 3'
Bsh1365IBsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
BshFIHaeIIIGGCCGG^CC
CC^GG
Blunt
BshGIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BshKIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BshNIHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
BshTIAgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
BsiICACGAGC^ACGAG
GTGCT^C
5' - TGCT
BsiBIBsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
BsiCIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
BsiEIMcrICGRYCGCGRY^CG
GC^RYGC
RY - 3'
BsiHKAIHgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
BsiHKCIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
BsiKIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
BsiLIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BsiMIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
BsiQIBclITGATCAT^GATCA
ACTAG^T
5' - CTAG
BsiSIHpaIICCGGC^CGG
GGC^C
5' - GC
BsiWISplICGTACGC^GTACG
GCATG^C
5' - CATG
BsiXIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BsiYICCNNNNNNNGGCCNNNNN^NNGG
GGNN^NNNNNCC
NNN - 3'
BsiZIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BslIBsiYICCNNNNNNNGGCCNNNNN^NNGG
GGNN^NNNNNCC
NNN - 3'
BslFIFinIGGGACGGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^
5' - NNNN
BsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
BsmAIGTCTCGTCTCN^NNNN
CAGAGNNNNN^
5' - NNNN
BsmBIEsp3ICGTCTCCGTCTCN^NNNN
GCAGAGNNNNN^
5' - NNNN
BsmFIFinIGGGACGGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^
5' - NNNN
BsmSIStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
Bso31IEco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
BsoBIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
BsoCISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
BsoFIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
BsoMAIBsmAIGTCTCGTCTCN^NNNN
CAGAGNNNNN^
5' - NNNN
Bsp6IFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
Bsp13IBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Bsp19INcoICCATGGC^CATGG
GGTAC^C
5' - GTAC
Bsp24IGACNNNNNNTGGGACNNNNNNTGGNNNNNNNNNNNN^
CTGNNNNNNACCNNNNNNN^NNNNN
NNNNN - 3'
Bsp50IFnuDIICGCGCG^CG
GC^GC
Blunt
Bsp63IPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Bsp67IMboIGATC^GATC
CTAG^
5' - CTAG
Bsp68INruITCGCGATCG^CGA
AGC^GCT
Blunt
Bsp98IBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
Bsp105IMboIGATC^GATC
CTAG^
5' - CTAG
Bsp106IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
Bsp119IAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Bsp120IApaIGGGCCCG^GGCCC
CCCGG^G
5' - CCGG
Bsp123IFnuDIICGCGCG^CG
GC^GC
Blunt
Bsp143IMboIGATC^GATC
CTAG^
5' - CTAG
Bsp143IIHaeIIRGCGCYRGCGC^Y
R^CGCGY
GCGC - 3'
Bsp211IHaeIIIGGCCGG^CC
CC^GG
Blunt
Bsp423IBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
Bsp519IHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
Bsp1286ISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
Bsp1407ITGTACAT^GTACA
ACATG^T
5' - CATG
Bsp1720IEspIGCTNAGCGC^TNAGC
CGANT^CG
5' - ANT
Bsp1894IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Bsp2095IMboIGATC^GATC
CTAG^
5' - CTAG
Bsp4009IBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
BspAIMboIGATC^GATC
CTAG^
5' - CTAG
BspA2IAvrIICCTAGGC^CTAGG
GGATC^C
5' - GATC
Bsp153AIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
BspAAIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
BspAAIIXbaITCTAGAT^CTAGA
AGATC^T
5' - GATC
BspAAIIIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
BspANIHaeIIIGGCCGG^CC
CC^GG
Blunt
BspBIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
BspBIIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BspBRIHaeIIIGGCCGG^CC
CC^GG
Blunt
BspBS31IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BspCIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
BspCNIBseMIICTCAGCTCAGNNNNNNNNN^
GAGTCNNNNNNN^NN
NN - 3'
BspDIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BspEIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
BspFIMboIGATC^GATC
CTAG^
5' - CTAG
BspF4IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
BspHITCATGAT^CATGA
AGTAC^T
5' - GTAC
BspIS4IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BspJIMboIGATC^GATC
CTAG^
5' - CTAG
BspJIIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BspKIHaeIIIGGCCGG^CC
CC^GG
Blunt
BspKT5IEco57ICTGAAGCTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN
NN - 3'
BspKT6IMboIGATCGAT^C
C^TAG
AT - 3'
BspKT8IHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
BspLINlaIVGGNNCCGGN^NCC
CCN^NGG
Blunt
BspLAIHhaIGCGCGCG^C
C^GCG
CG - 3'
BspLAIIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
BspLAIIIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
BspLS2ISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
BspLU4IAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
BspLU11IACATGTA^CATGT
TGTAC^A
5' - GTAC
BspLU11IIIFinIGGGACGGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^
5' - NNNN
BspMIACCTGCACCTGCNNNN^NNNN
TGGACGNNNNNNNN^
5' - NNNN
BspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
BspM39IPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
BspM90ISnaIGTATACGTA^TAC
CAT^ATG
Blunt
BspMAIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
BspMKISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
BspNIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BspO4IPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
BspOVIEam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
BspOVIIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BspPIBinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
BspRIHaeIIIGGCCGG^CC
CC^GG
Blunt
BspR7ISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
BspST5ISfaNIGCATCGCATCNNNNN^NNNN
CGTAGNNNNNNNNN^
5' - NNNN
BspTIAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
BspT104IAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
BspT107IHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
BspTNIEco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
BspTS514IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BspWIMwoIGCNNNNNNNGCGCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
BspXIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BspXIIBclITGATCAT^GATCA
ACTAG^T
5' - CTAG
BspZEIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BsrIACTGGACTGGN^
TGAC^CN
GN - 3'
BsrAIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
BsrBICCGCTCCCG^CTC
GGC^GAG
Blunt
BsrBRIBsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
BsrDIGCAATGGCAATGNN^
CGTTAC^NN
NN - 3'
BsrFICfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
BsrGIBsp1407ITGTACAT^GTACA
ACATG^T
5' - CATG
BsrSIBsrIACTGGACTGGN^
TGAC^CN
GN - 3'
BssAICfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
BssECISecICCNNGGC^CNNGG
GGNNC^C
5' - GNNC
BssHIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
BssHIIBsePIGCGCGCG^CGCGC
CGCGC^G
5' - GCGC
BssIMISimIGGGTCGG^GTC
CCCAG^
5' - CAG
BssKIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
BssNAISnaIGTATACGTA^TAC
CAT^ATG
Blunt
BssSIBsiICACGAGC^ACGAG
GTGCT^C
5' - TGCT
BssT1IStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
BstIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
Bst1IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bst2IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bst6IKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
Bst11IBsrIACTGGACTGGN^
TGAC^CN
GN - 3'
Bst12IBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
Bst19ISfaNIGCATCGCATCNNNN^NN
CGTAGNNNNNN^
5' - NN
Bst19IIMboIGATC^GATC
CTAG^
5' - CTAG
Bst28IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
Bst38IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bst40IHpaIICCGGC^CGG
GGC^C
5' - GC
Bst71IBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
Bst98IAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
Bst100IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bst1107ISnaIGTATACGTA^TAC
CAT^ATG
Blunt
BstACIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
BstAPIApaBIGCANNNNNTGCGCANNNN^NTGC
CGTN^NNNNACG
NNN - 3'
BstAUIBsp1407ITGTACAT^GTACA
ACATG^T
5' - CATG
BstBIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Bst2BIBsiICACGAGC^ACGAG
GTGCT^C
5' - TGCT
BstBAIBsaAIYACGTRYAC^GTR
YTG^CAR
Blunt
BstBSISnaIGTATACGTA^TAC
CAT^ATG
Blunt
BstB7SICfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
BstBS32IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BstBZ153IBsePIGCGCGCG^CGCGC
CGCGC^G
5' - GCGC
Bst4CITsp4CIACNGTACN^GT
TG^NCA
N - 3'
BstC8ICac8IGCNNGCGCN^NGC
CGN^NCG
Blunt
BstD102IBsrBICCGCTCCCG^CTC
GGC^GAG
Blunt
BstDEIDdeICTNAGC^TNAG
GANT^C
5' - ANT
BstDSIDsaICCRYGGC^CRYGG
GGRYC^C
5' - GRYC
BstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
BstENIEcoNICCTNNNNNAGGCCTNN^NNNAGG
GGANNN^NNTCC
5' - N
BstENIIMboIGATC^GATC
CTAG^
5' - CTAG
BstEZ359IHpaIGTTAACGTT^AAC
CAA^TTG
Blunt
BstFIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
BstF5IFokIGGATGGGATGNN^
CCTAC^NN
NN - 3'
BstFNIFnuDIICGCGCG^CG
GC^GC
Blunt
BstFZ438IFauICCCGCCCCGCNNNN^NN
GGGCGNNNNNN^
5' - NN
BstGZ53IEsp3ICGTCTCCGTCTCN^NNNN
GCAGAGNNNNN^
5' - NNNN
BstH2IHaeIIRGCGCYRGCGC^Y
R^CGCGY
GCGC - 3'
BstH9IBinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
BstHHIHhaIGCGCGCG^C
C^GCG
CG - 3'
BstHPIHpaIGTTAACGTT^AAC
CAA^TTG
Blunt
BstHZ55IBstXICCANNNNNNTGGCCANNNNN^NTGG
GGTN^NNNNNACC
NNNN - 3'
BstIZ316IDraIIICACNNNGTGCACNNN^GTG
GTG^NNNCAC
NNN - 3'
BstJZ301IDdeICTNAGC^TNAG
GANT^C
5' - ANT
BstKTIMboIGATCGAT^C
C^TAG
AT - 3'
BstM6IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BstMBIMboIGATC^GATC
CTAG^
5' - CTAG
BstMCIMcrICGRYCGCGRY^CG
GC^RYGC
RY - 3'
BstMWIMwoIGCNNNNNNNGCGCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
BstMZ611IScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
BstNIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Bst31NIBsrBICCGCTCCCG^CTC
GGC^GAG
Blunt
BstNSINspIRCATGYRCATG^Y
R^GTACY
CATG - 3'
BstNZ169IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BstOIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BstOZ616IFinIGGGACGGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^
5' - NNNN
BstPIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
BstPAIPshAIGACNNNNGTCGACNN^NNGTC
CTGNN^NNCAG
Blunt
BstPZ418IFokIGGATGGGATGNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNN^
5' - NNNN
BstPZ740IAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
BstRZ246ISwaIATTTAAATATTT^AAAT
TAAA^TTTA
Blunt
BstSIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
BstSCIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
BstSFISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
BstSNISnaBITACGTATAC^GTA
ATG^CAT
Blunt
BstSWISwaIATTTAAATATTT^AAAT
TAAA^TTTA
Blunt
BstT7IBclITGATCAT^GATCA
ACTAG^T
5' - CTAG
BstT9IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
BstT10IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
Bst31TIBinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
BstTS5IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BstUIFnuDIICGCGCG^CG
GC^GC
Blunt
Bst2UIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BstVIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
BstV1IBbvIGCAGCGCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^
5' - NNNN
BstV2IBbvIIGAAGACGAAGACNN^NNNN
CTTCTGNNNNNN^
5' - NNNN
BstXICCANNNNNNTGGCCANNNNN^NTGG
GGTN^NNNNNACC
NNNN - 3'
BstX2IXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
BstYIXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
BstZIXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
BstZ17ISnaIGTATACGTA^TAC
CAT^ATG
Blunt
Bsu6IKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
Bsu15IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
Bsu23IBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Bsu36ISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
Bsu54IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Bsu1532IFnuDIICGCGCG^CG
GC^GC
Blunt
Bsu1854IHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
BsuBIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
BsuFIHpaIICCGGC^CGG
GGC^C
5' - GC
BsuRIHaeIIIGGCCGG^CC
CC^GG
Blunt
BsuTUIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BteIHaeIIIGGCCGG^CC
CC^GG
Blunt
BtgIDsaICCRYGGC^CRYGG
GGRYC^C
5' - GRYC
BtgZIGCGATGGCGATGNNNNNNNNNN^NNNN
CGCTACNNNNNNNNNNNNNN^
5' - NNNN
BthAIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
BthCIFnu4HIGCNGCGCNG^C
C^GNCG
CNG - 3'
BthDIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BthEIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BtkIFnuDIICGCGCG^CG
GC^GC
Blunt
BtkIIMboIGATC^GATC
CTAG^
5' - CTAG
BtrICACGTCCAC^GTC
GTG^CAG
Blunt
BtsIGCAGTGGCAGTGNN^
CGTCAC^NN
NN - 3'
BveIBspMIACCTGCACCTGCNNNN^NNNN
TGGACGNNNNNNNN^
5' - NNNN
BvuIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
BvuBISplICGTACGC^GTACG
GCATG^C
5' - CATG
BaeGIBseSIGKGCMCGKGCM^C
C^KCGMG
KGCM - 3'
BciT130IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
BcoDIBsmAIGTCTCGTCTCN^NNNN
CAGAGNNNNN^
5' - NNNN
BisIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
BlsIGCNGCGCN^GC
CG^NCG
N - 3'
BmcAIScaIAGTACTAGT^ACT
TCA^TGA
Blunt
BmeRIAhdIGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
BmeT110ICYCGRGCY^CGRG
GYGC^RC
5' - GC
BmgT120IGGNCCGG^NCC
CCN^GG
5' - N
BmiIGGNNCCGGN^NCC
CCN^NGG
Blunt
BmrFICCNGGCC^NGG
GGN^CC
5' - N
BmuIACTGGG(NNNN)NACTGGG(NN^NN)N
TGACCC(N^NNN)N
N - 3'
BpuMICauIICCSGGCC^SGG
GGS^CC
5' - S
BpvUIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
BshVIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
BsnIHaeIIIGGCCGG^CC
CC^GG
Blunt
BspACIAciICCGCC^CGC
GGC^G
5' - GC
BspFNIFnuDIICGCGCG^CG
GC^GC
Blunt
BspOIGCTAGCGCTAG^C
C^GATCG
CTAG - 3'
BspQIGCTCTTCNNNNGCTCTTCN^NNN
CGAGAAGNNNN^
5' - NNN
BssMIMboIGATC^GATC
CTAG^
5' - CTAG
BssNIGRCGYCGR^CGYC
CRGC^YG
5' - GC
BstAFICTTAAGC^TTAAG
GAATT^C
5' - AATT
BstMAIGTCTCNNNNNGTCTCN^NNNN
CAGAGNNNNN^
5' - NNNN
BstSLIGKGCMCGKGCM^C
C^KCGMG
KGCM - 3'
BtsCIGGATGNNGGATGNN^
CCTAC^NN
NN - 3'
BtsIMutICAGTGCAGTGNN^
GTCAC^NN
NN - 3'
BtuMITCGCGATCG^CGA
TCG^CGA
Blunt
CacIMboIGATC^GATC
CTAG^
5' - CTAG
Cac8IGCNNGCGCN^NGC
CGN^NCG
Blunt
CaiIAlwNICAGNNNCTGCAGNNN^CTG
GTC^NNNGAC
NNN - 3'
CauIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
CauIICCSGGCC^SGG
GGS^CC
5' - S
CauB3IBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
CbiIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
CboIHpaIICCGGC^CGG
GGC^C
5' - GC
CbrIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
CciNINotIGCGGCCGCGC^GGCCGC
CGCCGG^CG
5' - CCGG
CcoINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
CcrIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
CcuIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
CcyIMboIGATC^GATC
CTAG^
5' - CTAG
CdiICATCGCATC^G
GTAG^C
Blunt
CelIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
CelIIEspIGCTNAGCGC^TNAGC
CGANT^CG
5' - ANT
CeqIEcoRVGATATCGAT^ATC
CTA^TAG
Blunt
CflIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
CfoIHhaIGCGCGCG^C
C^GCG
CG - 3'
CfrIYGGCCRY^GGCCR
YCCGG^R
5' - CCGG
Cfr6IPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
Cfr9ISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
Cfr10IRCCGGYR^CCGGY
RGGCC^Y
5' - GGCC
Cfr13IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Cfr42ISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
CfrA4IPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
CfrBIStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
CfrJ4ISmaICCCGGGCCC^GGG
GGG^CCC
Blunt
CfuIDpnIGATCGA^TC
CT^AG
Blunt
CfuIIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
ChaIMboIGATCGATC^
^CTAG
GATC - 3'
CjeICCANNNNNNGTCCANNNNNNGTNNNNNNNNNNNNNNN^
GGTNNNNNNCANNNNNNNNN^NNNNNN
NNNNNN - 3'
CjePICCANNNNNNNTCCCANNNNNNNTCNNNNNNNNNNNNNN^
GGTNNNNNNNAGNNNNNNNN^NNNNNN
NNNNNN - 3'
ClaIATCGATAT^CGAT
TAGC^TA
5' - GC
CltIHaeIIIGGCCGG^CC
CC^GG
Blunt
CpfIMboIGATC^GATC
CTAG^
5' - CTAG
CpoIRsrIICGGWCCGCG^GWCCG
GCCWG^GC
5' - CWG
CscISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
CsiAIAgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
CsiBINotIGCGGCCGCGC^GGCCGC
CGCCGG^CG
5' - CCGG
CspIRsrIICGGWCCGCG^GWCCG
GCCWG^GC
5' - CWG
Csp6IRsaIGTACG^TAC
CAT^G
5' - AT
Csp45IAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
CspAIAgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
CspBINotIGCGGCCGCGC^GGCCGC
CGCCGG^CG
5' - CCGG
CspCICAANNNNNGTGGCAANNNNNGTGGNNNNNNNNNNNN^
GTTNNNNNCACCNNNNNNNNNN^NN
NN - 3'
Csp68KIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
Csp68KIIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Csp68KIIIAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
Csp68KVIFnuDIICGCGCG^CG
GC^GC
Blunt
CspKVIFnuDIICGCGCG^CG
GC^GC
Blunt
CstIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
CstMIAAGGAGAAGGAGNNNNNNNNNNNNNNNNNNNN^
TTCCTCNNNNNNNNNNNNNNNNNN^NN
NN - 3'
CthIIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
CviAIMboIGATC^GATC
CTAG^
5' - CTAG
CviAIINlaIIICATGC^ATG
GTA^C
5' - TA
CviBIHinfIGANTCG^ANTC
CTNA^G
5' - TNA
CviJIRGCYRG^CY
RC^GY
Blunt
CviQIRsaIGTACG^TAC
CAT^G
5' - AT
CviRITGCATG^CA
AC^GT
Blunt
CviRIIRsaIGTACG^TAC
CAT^G
5' - AT
CviTICviJIRGCYRG^CY
RC^GY
Blunt
CvnISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
CciITCATGAT^CATGA
TCATG^A
5' - catg
CseIGACGCGACGCNNNNNN^NNNNN
GACGCNNNNNNNNNNN^
5' - NNNNN
CviKI-1RGCYRG^CY
RC^GY
Blunt
DdeICTNAGC^TNAG
GANT^C
5' - ANT
DmaIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
DpaIScaIAGTACTAGT^ACT
TCA^TGA
Blunt
DpnIGATCGA^TC
CT^AG
Blunt
DpnIIMboIGATC^GATC
CTAG^
5' - CTAG
DraIAhaIIITTTAAATTT^AAA
AAA^TTT
Blunt
DraIIRGGNCCYRG^GNCCY
RCCNG^GY
5' - CNG
DraIIICACNNNGTGCACNNN^GTG
GTG^NNNCAC
NNN - 3'
DrdIGACNNNNNNGTCGACNNNN^NNGTC
CTGNN^NNNNCAG
NN - 3'
DriIEam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
DsaICCRYGGC^CRYGG
GGRYC^C
5' - GRYC
DsaIIHaeIIIGGCCGG^CC
CC^GG
Blunt
DsaIIIXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
DsaIVAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
DsaVScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
DseDIDrdIGACNNNNNNGTCGACNNNN^NNGTC
CTGNN^NNNNCAG
NN - 3'
DinIGGCGCCGGC^GCC
CCG^CGG
Blunt
EacIBinIGGATCGGATCNNNN^N
CCTAGNNNNN^
5' - N
EaeICfrIYGGCCRY^GGCCR
YCCGG^R
5' - CCGG
Eae46ISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
EaeAISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
EagIXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
EagBIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
EagMIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
Eam1104IKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
Eam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
EarIKsp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
EcaIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
EciIGGCGGAGGCGGANNNNNNNNNNN^
CCGCCTNNNNNNNNN^NN
NN - 3'
Eci125IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
EclIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
Ecl136IISacIGAGCTCGAG^CTC
CTC^GAG
Blunt
EclHKIEam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
EclRISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
EclXIXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
Ecl18kIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
Ecl37kIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Ecl2zIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Eco24IHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
Eco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
Eco32IEcoRVGATATCGAT^ATC
CTA^TAG
Blunt
Eco47IAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
Eco47IIIAGCGCTAGC^GCT
TCG^CGA
Blunt
Eco52IXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
Eco56INaeIGCCGGCG^CCGGC
CGGCC^G
5' - GGCC
Eco57ICTGAAGCTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN
NN - 3'
Eco64IHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
Eco72IPmaCICACGTGCAC^GTG
GTG^CAC
Blunt
Eco78INarIGGCGCCGGC^GCC
CCG^CGG
Blunt
Eco81ISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
Eco88IAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
Eco91IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
Eco105ISnaBITACGTATAC^GTA
ATG^CAT
Blunt
Eco130IStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
Eco147IStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
Eco255IScaIAGTACTAGT^ACT
TCA^TGA
Blunt
Eco1831ICauIICCSGG^CCSGG
GGSCC^
5' - GGSCC
EcoA4IEco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
EcoHICauIICCSGG^CCSGG
GGSCC^
5' - GGSCC
EcoHK31ICfrIYGGCCRY^GGCCR
YCCGG^R
5' - CCGG
EcoICRISacIGAGCTCGAG^CTC
CTC^GAG
Blunt
Eco75KIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
Eco57MICTGRAGCTGRAGNNNNNNNNNNNNNNNN^
GACRTCNNNNNNNNNNNNNN^NN
NN - 3'
EcoNICCTNNNNNAGGCCTNN^NNNAGG
GGANNN^NNTCC
5' - N
EcoO44IEco31IGGTCTCGGTCTCN^NNNN
CCAGAGNNNNN^
5' - NNNN
EcoO65IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
EcoO109IDraIIRGGNCCYRG^GNCCY
RCCNG^GY
5' - CNG
EcoO128IBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
EcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
EcoRIICCWGG^CCWGG
GGWCC^
5' - GGWCC
EcoRVGATATCGAT^ATC
CTA^TAG
Blunt
EcoT14IStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
EcoT22IAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
EcoT38IHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
EcoVIIIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
Eco13kIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
Eco21kIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
Eco27kIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
Eco29kISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
Eco53kISacIGAGCTCGAG^CTC
CTC^GAG
Blunt
Eco137kIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
EgeINarIGGCGCCGGC^GCC
CCG^CGG
Blunt
EheINarIGGCGCCGGC^GCC
CCG^CGG
Blunt
ErhIStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
ErhB9IPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
ErhB9IIStyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
ErpIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
EsaBC3ITaqITCGATC^GA
AG^CT
Blunt
EsaBC4IHaeIIIGGCCGG^CC
CC^GG
Blunt
EspIGCTNAGCGC^TNAGC
CGANT^CG
5' - ANT
Esp3ICGTCTCCGTCTCN^NNNN
GCAGAGNNNNN^
5' - NNNN
Esp4IAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
Esp1396IPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
EcoP15ICAGCAGCAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN
GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN^
5' - NN
FalIAAGNNNNNCTTAAGNNNNNCTTNNNNNNNNNNNNN^
TTCNNNNNGAANNNNNNNN^NNNNN
NNNNN - 3'
FalIIFnuDIICGCGCG^CG
GC^GC
Blunt
FaqIFinIGGGACGGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^
5' - NNNN
FatINlaIIICATG^CATG
GTAC^
5' - GTAC
FauICCCGCCCCGCNNNN^NN
GGGCGNNNNNN^
5' - NN
FauBIIFnuDIICGCGCG^CG
GC^GC
Blunt
FauNDINdeICATATGCA^TATG
GTAT^AC
5' - AT
FbaIBclITGATCAT^GATCA
ACTAG^T
5' - CTAG
FblIAccIGTMKACGT^MKAC
CAMK^TG
5' - MK
FbrIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
FdiIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
FdiIIMstITGCGCATGC^GCA
ACG^CGT
Blunt
FgoIMaeICTAGC^TAG
GAT^C
5' - AT
FmuIAsuIGGNCCGGNC^C
C^CNGG
GNC - 3'
FnuAIHinfIGANTCG^ANTC
CTNA^G
5' - TNA
FnuCIMboIGATC^GATC
CTAG^
5' - CTAG
FnuDIHaeIIIGGCCGG^CC
CC^GG
Blunt
FnuDIICGCGCG^CG
GC^GC
Blunt
FnuDIIIHhaIGCGCGCG^C
C^GCG
CG - 3'
FnuEIMboIGATC^GATC
CTAG^
5' - CTAG
Fnu4HIGCNGCGC^NGC
CGN^CG
5' - N
FokIGGATGGGATGNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNN^
5' - NNNN
FriOIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
FseIGGCCGGCCGGCCGG^CC
CC^GGCCGG
CCGG - 3'
FsiIApoIRAATTYR^AATTY
RTTAA^Y
5' - TTAA
FspIMstITGCGCATGC^GCA
ACG^CGT
Blunt
FspIIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Fsp1604IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
FspAIRTGCGCAYRTGC^GCAY
RACG^CGTY
Blunt
FspBIMaeICTAGC^TAG
GAT^C
5' - AT
Fsp4HIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
FspMSIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
FssIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
FunIEco47IIIAGCGCTAGC^GCT
TCG^CGA
Blunt
FunIIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
FaeICATGCATG^
^GTAC
CATG - 3'
FaiIYATRYA^TR
YT^AR
Blunt
GalISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
GceISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
GceGLISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
GdiIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
GdiIICGGCCRC^GGCCR
GCCGG^R
5' - CCGG
GstIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
GsuICTGGAGCTGGAGNNNNNNNNNNNNNNNN^
GACCTCNNNNNNNNNNNNNN^NN
NN - 3'
GlaIGCGCGC^GC
CG^CG
Blunt
GluIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
GsaICCCAGCCCCAG^C
G^GGTCG
CCAG - 3'
HacIMboIGATC^GATC
CTAG^
5' - CTAG
HaeIWGGCCWWGG^CCW
WCC^GGW
Blunt
HaeIIRGCGCYRGCGC^Y
R^CGCGY
GCGC - 3'
HaeIIIGGCCGG^CC
CC^GG
Blunt
HaeIVGAYNNNNNRTCGAYNNNNNRTCNNNNNNNNNNNNNN^
CTYNNNNNRAGNNNNNNNNN^NNNNN
NNNNN - 3'
HalIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
HalIIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
HapIIHpaIICCGGC^CGG
GGC^C
5' - GC
HgaIGACGCGACGCNNNNN^NNNNN
CTGCGNNNNNNNNNN^
5' - NNNNN
HgiIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
HgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
HgiBIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
HgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
HgiCIIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
HgiCIIISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
HgiDIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
HgiDIISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
HgiEIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
HgiGIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
HgiHIHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
HgiHIIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
HgiHIIIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
HgiJIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
HgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
HgiS22ICauIICCSGGCC^SGG
GGS^CC
5' - S
HhaIGCGCGCG^C
C^GCG
CG - 3'
HhaIIHinfIGANTCG^ANTC
CTNA^G
5' - TNA
Hin1IAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
Hin1IINlaIIICATGCATG^
^GTAC
CATG - 3'
Hin2IHpaIICCGGC^CGG
GGC^C
5' - GC
Hin4IGAYNNNNNVTCGAYNNNNNVTCNNNNNNNNNNNNN^
CTYNNNNNVAGNNNNNNNN^NNNNN
NNNNN - 3'
Hin6IHhaIGCGCG^CGC
CGC^G
5' - GC
HinJCIHindIIGTYRACGTY^RAC
CAY^RTG
Blunt
HinP1IHhaIGCGCG^CGC
CGC^G
5' - GC
HincIIHindIIGTYRACGTY^RAC
CAY^RTG
Blunt
HindIIGTYRACGTY^RAC
CAY^RTG
Blunt
HindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
HinfIGANTCG^ANTC
CTNA^G
5' - TNA
HjaIEcoRVGATATCGAT^ATC
CTA^TAG
Blunt
HpaIGTTAACGTT^AAC
CAA^TTG
Blunt
HpaIICCGGC^CGG
GGC^C
5' - GC
HphIGGTGAGGTGANNNNNNNN^
CCACTNNNNNNN^N
N - 3'
Hpy8IMjaIVGTNNACGTN^NAC
CAN^NTG
Blunt
Hpy51ITsp45IGTSAC^GTSAC
CASTG^
5' - CASTG
Hpy99ICGWCGCGWCG^
^GCWGC
CGWCG - 3'
Hpy178IIITCNNGATC^NNGA
AGNN^CT
5' - NN
Hpy188ITCNGATCN^GA
AG^NCT
N - 3'
Hpy188IIIHpy178IIITCNNGATC^NNGA
AGNN^CT
5' - NN
HpyAVHin4IICCTTCCCTTCNNNNNN^
GGAAGNNNNN^N
N - 3'
HpyBIRsaIGTACGT^AC
CA^TG
Blunt
HpyBIIMjaIVGTNNACGTN^NAC
CAN^NTG
Blunt
HpyCIEcoRVGATATCGAT^ATC
CTA^TAG
Blunt
HpyC1IBccICCATCCCATCNNNN^N
GGTAGNNNNN^
5' - N
HpyCH4INlaIIICATGCATG^
^GTAC
CATG - 3'
HpyCH4IIITsp4CIACNGTACN^GT
TG^NCA
N - 3'
HpyCH4IVMaeIIACGTA^CGT
TGC^A
5' - GC
HpyCH4VCviRITGCATG^CA
AC^GT
Blunt
HpyF10VIMwoIGCNNNNNNNGCGCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
HpyF44IIICviRITGCATG^CA
AC^GT
Blunt
HsoIHhaIGCGCG^CGC
CGC^G
5' - GC
Hsp92IAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
Hsp92IINlaIIICATGCATG^
^GTAC
CATG - 3'
HspAIHhaIGCGCG^CGC
CGC^G
5' - GC
HsuIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
Hpy166IIMjaIVGTNNACGTN^NAC
CAN^NTG
Blunt
HpyF3IDdeICTNAGC^TNAG
GANT^C
5' - ANT
ItaIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
I-CeuITAACTATAACGGTCCTAAGGTAGCGATAACTATAACGGTCCTAA^GGTAGCGA
ATTGATATTGCCAG^GATTCCATCGCT
CTAA - 3'
I-PpoITAACTATGACTCTCTTAAGGTAGCCAAATTAACTATGACT^CTCTTAAGGTAGCCAAAT
ATTGATACTGAGAGAATT^CCATCGGTTTA
5' - GAGAATT
I-SceITAGGGATAACAGGGTAATTAGGGATAA^CAGGGTAAT
ATCCC^TATTGTCCCATTA
ATAA - 3'
KasINarIGGCGCCG^GCGCC
CCGCG^G
5' - CGCG
Kaz48kIDraIIRGGNCCYRGGNC^CY
RC^CNGGY
GNC - 3'
KoxIIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
KpnIGGTACCGGTAC^C
C^CATGG
GTAC - 3'
Kpn2IBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Kpn378ISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
Kpn2kIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
Kpn49kIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
Kpn49kIICauIICCSGG^CCSGG
GGSCC^
5' - GGSCC
KspISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
Ksp22IBclITGATCAT^GATCA
ACTAG^T
5' - CTAG
Ksp632ICTCTTCCTCTTCN^NNN
GAGAAGNNNN^
5' - NNN
KspAIHpaIGTTAACGTT^AAC
CAA^TTG
Blunt
Kzo9IMboIGATC^GATC
CTAG^
5' - CTAG
Kzo49IAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
LcaIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
LlaAIMboIGATC^GATC
CTAG^
5' - CTAG
LlaBISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
LlaCIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
LlaG2INheIGCTAGCG^CTAGC
CGATC^G
5' - GATC
Lmu60ISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
LplIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
LpnIHaeIIRGCGCYRGC^GCY
RCG^CGY
Blunt
LspIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
LweISfaNIGCATCGCATCNNNNN^NNNN
CGTAGNNNNNNNNN^
5' - NNNN
LguIGCTCTTCNNNNGCTCTTCN^NNN
CGA^GAAGNNNN
CTTCN - 3'
Lsp1109IGCAGC(N)8(N)4GCAGC(N)8(N)^4
CGTC^G(N)8(N)4
C(N)8(N) - 3'
MabISexAIACCWGGTA^CCWGGT
TGGWCC^A
5' - GGWCC
MaeICTAGC^TAG
GAT^C
5' - AT
MaeIIACGTA^CGT
TGC^A
5' - GC
MaeIIIGTNAC^GTNAC
CANTG^
5' - CANTG
MaeK81ISplICGTACGC^GTACG
GCATG^C
5' - CATG
MaeK81IIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
MamIBsaBIGATNNNNATCGATNN^NNATC
CTANN^NNTAG
Blunt
MavIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
MbiIBsrBICCGCTCCCG^CTC
GGC^GAG
Blunt
MboIGATC^GATC
CTAG^
5' - CTAG
MboIIGAAGAGAAGANNNNNNNN^
CTTCTNNNNNNN^N
N - 3'
MchINarIGGCGCCGG^CGCC
CCGC^GG
5' - GC
MchAINotIGCGGCCGCGC^GGCCGC
CGCCGG^CG
5' - CCGG
MchAIIHaeIIIGGCCGG^CC
CC^GG
Blunt
McrICGRYCGCGRY^CG
GC^RYGC
RY - 3'
MfeICAATTGC^AATTG
GTTAA^C
5' - TTAA
MflIXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
MfoAIHaeIIIGGCCGG^CC
CC^GG
Blunt
Mgl14481ICauIICCSGGCC^SGG
GGS^CC
5' - S
MgoIMboIGATC^GATC
CTAG^
5' - CTAG
MhaAIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
MhlISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
MkrAIMboIGATC^GATC
CTAG^
5' - CTAG
MlaIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
MlaAIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
MlsIBalITGGCCATGG^CCA
ACC^GGT
Blunt
MltIAluIAGCTAG^CT
TC^GA
Blunt
MluIACGCGTA^CGCGT
TGCGC^A
5' - GCGC
Mlu23IBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
Mlu31IBalITGGCCATGG^CCA
ACC^GGT
Blunt
MluB2INruITCGCGATCG^CGA
AGC^GCT
Blunt
MluNIBalITGGCCATGG^CCA
ACC^GGT
Blunt
MlyIPleIGAGTCGAGTCNNNNN^
CTCAGNNNNN^
Blunt
Mly113INarIGGCGCCGG^CGCC
CCGC^GG
5' - GC
MmeITCCRACTCCRACNNNNNNNNNNNNNNNNNNNN^
AGGRTGNNNNNNNNNNNNNNNNNN^NN
NN - 3'
MnlICCTCCCTCNNNNNNN^
GGAGNNNNNN^N
N - 3'
MnoIHpaIICCGGC^CGG
GGC^C
5' - GC
Mph1103IAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
MroIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
MroNINaeIGCCGGCG^CCGGC
CGGCC^G
5' - GGCC
MroXIXmnIGAANNNNTTCGAANN^NNTTC
CTTNN^NNAAG
Blunt
MscIBalITGGCCATGG^CCA
ACC^GGT
Blunt
MseITTAAT^TAA
AAT^T
5' - AT
MslICAYNNNNRTGCAYNN^NNRTG
GTYNN^NNRAC
Blunt
MspIHpaIICCGGC^CGG
GGC^C
5' - GC
Msp17IAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
Msp20IBalITGGCCATGG^CCA
ACC^GGT
Blunt
Msp67IScrFICCNGGCC^NGG
GGN^CC
5' - N
MspA1INspBIICMGCKGCMG^CKG
GMC^GKC
Blunt
MspB4IHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
MspCIAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
MspR9IScrFICCNGGCC^NGG
GGN^CC
5' - N
MspSWISwaIATTTAAATATTT^AAAT
TAAA^TTTA
Blunt
MspV281IHgiAIGWGCWCGWGCW^C
C^WCGWG
WGCW - 3'
MspYIBsaAIYACGTRYAC^GTR
YTG^CAR
Blunt
MssIPmeIGTTTAAACGTTT^AAAC
CAAA^TTTG
Blunt
MstITGCGCATGC^GCA
ACG^CGT
Blunt
MstIISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
MthZIMaeICTAGC^TAG
GAT^C
5' - AT
MunIMfeICAATTGC^AATTG
GTTAA^C
5' - TTAA
MvaIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Mva1269IBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
MvnIFnuDIICGCGCG^CG
GC^GC
Blunt
MvrIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
MwoIGCNNNNNNNGCGCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
MxaISacIGAGCTCGAG^CTC
CTC^GAG
Blunt
MalIMboIGATCGA^TC
CT^AG
Blunt
MauBICGCGCGCGCG^CGCGCG
GCGCGC^GC
5' - GCGC
MluCITspEIAATT^AATT
TTAA^
5' - TTAA
MreISse232ICGCCGGCGCG^CCGGCG
GCGGCC^GC
5' - GGCC
NaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
NarIGGCGCCGG^CGCC
CCGC^GG
5' - GC
NblIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
NciICauIICCSGGCC^SGG
GGS^CC
5' - S
NcoICCATGGC^CATGG
GGTAC^C
5' - GTAC
NcrIBglIIAGATCTA^GATCT
TCTAG^A
5' - CTAG
NcuIMboIIGAAGAGAAGANNNNNNNN^
CTTCTNNNNNNN^N
N - 3'
NdaINarIGGCGCCGG^CGCC
CCGC^GG
5' - GC
NdeICATATGCA^TATG
GTAT^AC
5' - AT
NdeIIMboIGATC^GATC
CTAG^
5' - CTAG
NgoAIIISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
NgoAIVNaeIGCCGGCG^CCGGC
CGGCC^G
5' - GGCC
NgoMIVNaeIGCCGGCG^CCGGC
CGGCC^G
5' - GGCC
NgoPIIHaeIIIGGCCGG^CC
CC^GG
Blunt
NgoPIIISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
NheIGCTAGCG^CTAGC
CGATC^G
5' - GATC
NlaIIMboIGATC^GATC
CTAG^
5' - CTAG
NlaIIICATGCATG^
^GTAC
CATG - 3'
NlaIVGGNNCCGGN^NCC
CCN^NGG
Blunt
Nli3877IAvaICYCGRGCYCGR^G
G^YGCRC
YCGR - 3'
NmeCIMboIGATC^GATC
CTAG^
5' - CTAG
NmeRIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
NmuCITsp45IGTSAC^GTSAC
CASTG^
5' - CASTG
NopISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
NotIGCGGCCGCGC^GGCCGC
CGCCGG^CG
5' - CCGG
NphIMboIGATC^GATC
CTAG^
5' - CTAG
NruITCGCGATCG^CGA
AGC^GCT
Blunt
NruGIEam1105IGACNNNNNGTCGACNNN^NNGTC
CTGNN^NNNCAG
N - 3'
NsbIMstITGCGCATGC^GCA
ACG^CGT
Blunt
NsiIAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
NsiCIEcoRVGATATCGAT^ATC
CTA^TAG
Blunt
NspIRCATGYRCATG^Y
R^GTACY
CATG - 3'
NspIISduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
NspIIIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
NspIVAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
NspVAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Nsp7121IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Nsp29132IIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
NspBIICMGCKGCMG^CKG
GMC^GKC
Blunt
NspHINspIRCATGYRCATG^Y
R^GTACY
CATG - 3'
NspLKIHaeIIIGGCCGG^CC
CC^GG
Blunt
NspMACIBglIIAGATCTA^GATCT
TCTAG^A
5' - CTAG
NspSAIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
NspSAIIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
NspSAIVBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
NunIINarIGGCGCCGG^CGCC
CCGC^GG
5' - GC
NmeAIIIGCCGAGGCCGAGNNNNNNNNNNNNNNNNNNNN^
CGGCTCNNNNNNNNNNNNNNNNNN^NN
NN - 3'
OfoIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
OkrAIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
OliICACNNNNGTGCACNN^NNGTG
GTGNN^NNCAC
Blunt
OxaNISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
PacITTAATTAATTAAT^TAA
AAT^TAATT
AT - 3'
Pac25ISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
PaeISphIGCATGCGCATG^C
C^GTACG
CATG - 3'
PaeAISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
PaeBISmaICCCGGGCCC^GGG
GGG^CCC
Blunt
PaeHIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
PaePIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
PaeQISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
PaeR7IXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
Pae2kIBglIIAGATCTA^GATCT
TCTAG^A
5' - CTAG
Pae5kISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
Pae14kISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
Pae17kIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
Pae18kIBglIIAGATCTA^GATCT
TCTAG^A
5' - CTAG
PagIBspHITCATGAT^CATGA
AGTAC^T
5' - GTAC
PalIHaeIIIGGCCGG^CC
CC^GG
Blunt
PamIMstITGCGCATGC^GCA
ACG^CGT
Blunt
PamIIAcyIGRCGYCGR^CGYC
CRGC^YG
5' - GC
PanIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
PasICCCWGGGCC^CWGGG
GGGWC^CC
5' - GWC
PauIBsePIGCGCGCG^CGCGC
CGCGC^G
5' - GCGC
PauAINspIRCATGYRCATG^Y
R^GTACY
CATG - 3'
PauAIIAhaIIITTTAAATTT^AAA
AAA^TTT
Blunt
PceIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
PciIBspLU11IACATGTA^CATGT
TGTAC^A
5' - GTAC
PctIBsmIGAATGCGAATGCN^
CTTAC^GN
CN - 3'
Pde12IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Pde133IHaeIIIGGCCGG^CC
CC^GG
Blunt
Pde137IHpaIICCGGC^CGG
GGC^C
5' - GC
PdiINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
PdmIXmnIGAANNNNTTCGAANN^NNTTC
CTTNN^NNAAG
Blunt
PfaAIHgiCIGGYRCCG^GYRCC
CCYRG^G
5' - CYRG
PfaAIINdeICATATGCA^TATG
GTAT^AC
5' - AT
PfaAIIISphIGCATGCGCATG^C
C^GTACG
CATG - 3'
PfeITfiIGAWTCG^AWTC
CTWA^G
5' - TWA
Pfl8IBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
Pfl21IPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Pfl23IISplICGTACGC^GTACG
GCATG^C
5' - CATG
Pfl27IPpuMIRGGWCCYRG^GWCCY
RCCWG^GY
5' - CWG
PflBIPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
PflFITth111IGACNNNGTCGACN^NNGTC
CTGNN^NCAG
5' - N
PflKIHaeIIIGGCCGG^CC
CC^GG
Blunt
PflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
PfoITCCNGGAT^CCNGGA
AGGNCC^T
5' - GGNCC
PgaIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
PhaISfaNIGCATCGCATCNNNNN^NNNN
CGTAGNNNNNNNNN^
5' - NNNN
PhoIHaeIIIGGCCGG^CC
CC^GG
Blunt
PinAIAgeIACCGGTA^CCGGT
TGGCC^A
5' - GGCC
PinBIAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
PinBIIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
PlaIHaeIIIGGCCGG^CC
CC^GG
Blunt
PlaIIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
PlaAIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
PlaAIIRsaIGTACGT^AC
CA^TG
Blunt
PleIGAGTCGAGTCNNNN^N
CTCAGNNNNN^
5' - N
Ple19IPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
PmaCICACGTGCAC^GTG
GTG^CAC
Blunt
PmeIGTTTAAACGTTT^AAAC
CAAA^TTTG
Blunt
Pme55IStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
PmlIPmaCICACGTGCAC^GTG
GTG^CAC
Blunt
PpaAIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
PpaAIITaqITCGAT^CGA
AGC^T
5' - GC
PpeIApaIGGGCCCGGGCC^C
C^CCGGG
GGCC - 3'
PpiIGAACNNNNNCTCGAACNNNNNCTCNNNNNNNNNNNNN^
CTTGNNNNNGAGNNNNNNNN^NNNNN
NNNNN - 3'
PpsIPleIGAGTCGAGTCNNNN^N
CTCAGNNNNN^
5' - N
Ppu10IAvaIIIATGCATA^TGCAT
TACGT^A
5' - ACGT
Ppu111IEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
PpuAISplICGTACGC^GTACG
GCATG^C
5' - CATG
PpuMIRGGWCCYRG^GWCCY
RCCWG^GY
5' - CWG
PpuXIPpuMIRGGWCCYRG^GWCCY
RCCWG^GY
5' - CWG
PshAIGACNNNNGTCGACNN^NNGTC
CTGNN^NNCAG
Blunt
PshBIVspIATTAATAT^TAAT
TAAT^TA
5' - AT
PsiITTATAATTA^TAA
AAT^ATT
Blunt
Psp03IAvaIIGGWCCGGWC^C
C^CWGG
GWC - 3'
Psp5IIPpuMIRGGWCCYRG^GWCCY
RCCWG^GY
5' - CWG
Psp6IEcoRIICCWGG^CCWGG
GGWCC^
5' - GGWCC
Psp23IPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Psp1406IAclIAACGTTAA^CGTT
TTGC^AA
5' - GC
PspAISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
PspALISmaICCCGGGCCC^GGG
GGG^CCC
Blunt
Psp124BISacIGAGCTCGAGCT^C
C^TCGAG
AGCT - 3'
PspCIPmaCICACGTGCAC^GTG
GTG^CAC
Blunt
PspEIBstEIIGGTNACCG^GTNACC
CCANTG^G
5' - CANTG
PspGIEcoRIICCWGG^CCWGG
GGWCC^
5' - GGWCC
PspLISplICGTACGC^GTACG
GCATG^C
5' - CATG
PspN4INlaIVGGNNCCGGN^NCC
CCN^NGG
Blunt
PspOMIApaIGGGCCCG^GGCCC
CCCGG^G
5' - CCGG
PspPIAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
PspPPIPpuMIRGGWCCYRG^GWCCY
RCCWG^GY
5' - CWG
PspXIVCTCGAGBVC^TCGAGB
VGAGCT^CB
5' - AGCT
PsrIGAACNNNNNNTACGAACNNNNNNTACNNNNNNNNNNNN^
CTTGNNNNNNATGNNNNNNN^NNNNN
NNNNN - 3'
PssIDraIIRGGNCCYRGGNC^CY
RC^CNGGY
GNC - 3'
PstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
PstNHINheIGCTAGCG^CTAGC
CGATC^G
5' - GATC
PsuIXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
Psu161IPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
PsuAIBsaAIYACGTRYAC^GTR
YTG^CAR
Blunt
PsyITth111IGACNNNGTCGACN^NNGTC
CTGNN^NCAG
5' - N
PtaIBspMIITCCGGAT^CCGGA
AGGCC^T
5' - GGCC
Pun14627IMstITGCGCATGC^GCA
ACG^CGT
Blunt
Pun14627IIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
PunAIAvaICYCGRGC^YCGRG
GYGCR^C
5' - YGCR
PunAIINspIRCATGYRCATG^Y
R^GTACY
CATG - 3'
PvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
PvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
Pvu84IIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
PI-SceIATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCAATCTATGTCGGGTGC^GGAGAAAGAGGTAATGAAATGGCA
TAGATACAGCC^CACGCCTCTTTCTCCATTACTTTACCGT
GTGC - 3'
PalAIAscIGGCGCGCCGG^CGCGCC
CCGCGC^GG
5' - GCGC
PciSISapIGCTCTTCGCTCTTCN^NNN
CGAGAAGNNNN^
5' - NNN
PI-PspITGGCAAACAGCTATTATGGGTATTATGGGTTGGCAAACAGCTATTAT^GGGTATTATGGGT
ACCGTTTGTCGAT^AATACCCATAATACCCA
TTAT - 3'
PluTINarIGGCGCCGGCGC^C
C^CGCGG
GCGC - 3'
Ppu21IYACGTRYAC^GTR
YTG^CAR
Blunt
PscIACATGTA^CATGT
TGTAC^A
5' - GTAC
PspFICCCAGCCCCAG^C
G^GGTCG
CCAG - 3'
RalF40IMboIGATC^GATC
CTAG^
5' - CTAG
RcaIBspHITCATGAT^CATGA
AGTAC^T
5' - GTAC
RflFISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
RflFIIScaIAGTACTAGT^ACT
TCA^TGA
Blunt
RleAICCCACACCCACANNNNNNNNNNNN^
GGGTGTNNNNNNNNN^NNN
NNN - 3'
RmaIMaeICTAGC^TAG
GAT^C
5' - AT
Rme21IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
RsaIGTACGT^AC
CA^TG
Blunt
RshIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
RspLKISphIGCATGCGCATG^C
C^GTACG
CATG - 3'
RspLKIIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
RspXIBspHITCATGAT^CATGA
AGTAC^T
5' - GTAC
RsrIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
RsrIICGGWCCGCG^GWCCG
GCCWG^GC
5' - CWG
Rsr2IRsrIICGGWCCGCG^GWCCG
GCCWG^GC
5' - CWG
RtrISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
Rtr63ISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
RgaIGCGATCGCGCGAT^CGC
CGC^TAGCG
AT - 3'
RigIGGCCGGCCGGCCGG^CC
CC^GGCCGG
CCGG - 3'
RsaNIGTACG^TAC
CAT^G
5' - AT
RseICAYNNNNRTGCAYNN^NNRTG
GTYNN^NNRAC
Blunt
SacIGAGCTCGAGCT^C
C^TCGAG
AGCT - 3'
SacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SacNIHgiJIIGRGCYCGRGCY^C
C^RCGYG
RGCY - 3'
SalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
SalPIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
SanDIGGGWCCCGG^GWCCC
CCCWG^GG
5' - CWG
SapIGCTCTTCGCTCTTCN^NNN
CGAGAAGNNNN^
5' - NNN
SarIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
SatIFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
SauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
Sau96IAsuIGGNCCG^GNCC
CCNG^G
5' - CNG
Sau3239IXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
Sau3AIMboIGATC^GATC
CTAG^
5' - CTAG
SauBMKINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SauHPINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SauLPINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SauLPIIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
SauMIMboIGATC^GATC
CTAG^
5' - CTAG
SauNINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SauSINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SbfISse8387ICCTGCAGGCCTGCA^GG
GG^ACGTCC
TGCA - 3'
Sbi68IXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
Sbo13INruITCGCGATCG^CGA
AGC^GCT
Blunt
SbvIHaeIIIGGCCGG^CC
CC^GG
Blunt
ScaIAGTACTAGT^ACT
TCA^TGA
Blunt
SceIIINaeIGCCGGCG^CCGGC
CGGCC^G
5' - GGCC
SchIPleIGAGTCGAGTCNNNNN^
CTCAGNNNNN^
Blunt
SchZISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SciIXhoICTCGAGCTC^GAG
GAG^CTC
Blunt
SciNIHhaIGCGCG^CGC
CGC^G
5' - GC
ScrFICCNGGCC^NGG
GGN^CC
5' - N
SdaISse8387ICCTGCAGGCCTGCA^GG
GG^ACGTCC
TGCA - 3'
SdiISfiIGGCCNNNNNGGCCGGCCNNNN^NGGCC
CCGGN^NNNNCCGG
NNN - 3'
SduIGDGCHCGDGCH^C
C^DCGHG
DGCH - 3'
SecICCNNGGC^CNNGG
GGNNC^C
5' - GNNC
SelIFnuDIICGCG^CGCG
GCGC^
5' - GCGC
SenPT16IXmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
SenPT14bISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SepIAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
SexAIACCWGGTA^CCWGGT
TGGWCC^A
5' - GGWCC
SexBISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SexCISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SfaIHaeIIIGGCCGG^CC
CC^GG
Blunt
SfaNIGCATCGCATCNNNNN^NNNN
CGTAGNNNNNNNNN^
5' - NNNN
SfcISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
SfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
SfiIGGCCNNNNNGGCCGGCCNNNN^NGGCC
CCGGN^NNNNCCGG
NNN - 3'
SflIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
SfoINarIGGCGCCGGC^GCC
CCG^CGG
Blunt
Sfr274IXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
Sfr303ISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SfuIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
SgfIGCGATCGCGCGAT^CGC
CGC^TAGCG
AT - 3'
SgrAICRCCGGYGCR^CCGGYG
GRGGCC^YC
5' - GGCC
SgrBISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SimIGGGTCGG^GTC
CCCAG^
5' - CAG
SinIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
SlaIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
SleIEcoRIICCWGG^CCWGG
GGWCC^
5' - GGWCC
Slu1777INaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SmaICCCGGGCCC^GGG
GGG^CCC
Blunt
SmiISwaIATTTAAATATTT^AAAT
TAAA^TTTA
Blunt
SmiMIMslICAYNNNNRTGCAYNN^NNRTG
GTYNN^NNRAC
Blunt
SmlICTYRAGC^TYRAG
GAYRT^C
5' - AYRT
SmuIFauICCCGCCCCGCNNNN^NN
GGGCGNNNNNN^
5' - NN
SmuEIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
SnaBITACGTATAC^GTA
ATG^CAT
Blunt
SniIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
SnoIApaLIGTGCACG^TGCAC
CACGT^G
5' - ACGT
SolIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
Sol10179IXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
SpaHISphIGCATGCGCATG^C
C^GTACG
CATG - 3'
SpeIACTAGTA^CTAGT
TGATC^A
5' - GATC
SphIGCATGCGCATG^C
C^GTACG
CATG - 3'
SplICGTACGC^GTACG
GCATG^C
5' - CATG
SpmIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
SpoINruITCGCGATCG^CGA
AGC^GCT
Blunt
SpuISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
SrfIGCCCGGGCGCCC^GGGC
CGGG^CCCG
Blunt
SrlINaeIGCCGGCG^CCGGC
CGGCC^G
5' - GGCC
Srl5DIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
Srl32DIIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
Srl55DIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
Srl56DISfeICTRYAGC^TRYAG
GARYT^C
5' - ARYT
SruIAhaIIITTTAAATTT^AAA
AAA^TTT
Blunt
Sru4DIVspIATTAATAT^TAAT
TAAT^TA
5' - AT
Sru30DIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
SsbIHindIIIAAGCTTA^AGCTT
TTCGA^A
5' - TCGA
SscL1IHinfIGANTCG^ANTC
CTNA^G
5' - TNA
Sse9ITspEIAATT^AATT
TTAA^
5' - TTAA
Sse232ICGCCGGCGCG^CCGGCG
GCGGCC^GC
5' - GGCC
Sse1825ISanDIGGGWCCCGG^GWCCC
CCCWG^GG
5' - CWG
Sse8387ICCTGCAGGCCTGCA^GG
GG^ACGTCC
TGCA - 3'
Sse8647IAGGWCCTAG^GWCCT
TCCWG^GA
5' - CWG
SseAINarIGGCGCCGG^CGCC
CCGC^GG
5' - GC
SseBIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
SshAISauICCTNAGGCC^TNAGG
GGANT^CC
5' - ANT
SsiIAciICCGCC^CGC
GGC^G
5' - GC
SsiAIMboIGATC^GATC
CTAG^
5' - CTAG
SsiBIMboIGATC^GATC
CTAG^
5' - CTAG
SslIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
SsoIEcoRIGAATTCG^AATTC
CTTAA^G
5' - TTAA
SsoIIScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
SspIAATATTAAT^ATT
TTA^TAA
Blunt
Ssp1IAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
Ssp4800IBsp1407ITGTACAT^GTACA
ACATG^T
5' - CATG
Ssp5230IAatIIGACGTCGACGT^C
C^TGCAG
ACGT - 3'
Ssp27144IClaIATCGATAT^CGAT
TAGC^TA
5' - GC
SspAIEcoRIICCWGG^CCWGG
GGWCC^
5' - GGWCC
SspBIBsp1407ITGTACAT^GTACA
ACATG^T
5' - CATG
SspCINaeIGCCGGCGCC^GGC
CGG^CCG
Blunt
SspD5IHphIGGTGAGGTGANNNNNNNN^
CCACTNNNNNNNN^
Blunt
SspD5IIAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'
SspRFIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
SsrIHpaIGTTAACGTT^AAC
CAA^TTG
Blunt
SstISacIGAGCTCGAGCT^C
C^TCGAG
AGCT - 3'
SstIISacIICCGCGGCCGC^GG
GG^CGCC
GC - 3'
Sst12IPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
SteIStuIAGGCCTAGG^CCT
TCC^GGA
Blunt
SthIKpnIGGTACCG^GTACC
CCATG^G
5' - CATG
Sth117IEcoRIICCWGGCC^WGG
GGW^CC
5' - W
Sth132ICCCGCCCGNNNN^NNNN
GGGCNNNNNNNN^
5' - NNNN
Sth134IHpaIICCGGC^CGG
GGC^C
5' - GC
Sth368IMboIGATC^GATC
CTAG^
5' - CTAG
StrIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
StsIFokIGGATGGGATGNNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNNN^
5' - NNNN
StuIAGGCCTAGG^CCT
TCC^GGA
Blunt
StyICCWWGGC^CWWGG
GGWWC^C
5' - GWWC
StyD4IScrFICCNGG^CCNGG
GGNCC^
5' - GGNCC
SuaIHaeIIIGGCCGG^CC
CC^GG
Blunt
SunISplICGTACGC^GTACG
GCATG^C
5' - CATG
SurIBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
SviIAsuIITTCGAATT^CGAA
AAGC^TT
5' - GC
SwaIATTTAAATATTT^AAAT
TAAA^TTTA
Blunt
SetIASSTASST^
^TSSA
ASST - 3'
SfaAIGCGATCGCGCGAT^CGC
CGC^TAGCG
AT - 3'
SgrDICGTCGACGCG^TCGACG
GCAGCT^GC
5' - AGCT
SgsIGGCGCGCCGG^CGCGCC
CCGCGC^GG
5' - GCGC
SmoICTYRAGC^TYRAG
GAYRT^C
5' - AYRT
SspDIGGCGCCG^GCGCC
CCGCG^G
5' - CGCG
TaaITsp4CIACNGTACN^GT
TG^NCA
N - 3'
TaiIMaeIIACGTACGT^
^TGCA
ACGT - 3'
TaqITCGAT^CGA
AGC^T
5' - GC
TaqIIGACCGACACCCAGACCGACACCCANNNNNNNNNNN^
CTGGCTGTGGGTNNNNNNNNN^NN
NN - 3'
Taq52ITseIGCWGCG^CWGC
CGWC^G
5' - GWC
TaqXIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
TasITspEIAATT^AATT
TTAA^
5' - TTAA
TatIWGTACWW^GTACW
WCATG^W
5' - CATG
TauIGCSGCGCSG^C
C^GSCG
CSG - 3'
TelITth111IGACNNNGTCGACN^NNGTC
CTGNN^NCAG
5' - N
TfiIGAWTCG^AWTC
CTWA^G
5' - TWA
ThaIFnuDIICGCGCG^CG
GC^GC
Blunt
TliIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
Tru1IMseITTAAT^TAA
AAT^T
5' - AT
Tru9IMseITTAAT^TAA
AAT^T
5' - AT
Tru201IXhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
TscIMaeIIACGTACGT^
^TGCA
ACGT - 3'
TseIGCWGCG^CWGC
CGWC^G
5' - GWC
Tsp1IBsrIACTGGACTGGN^
TGAC^CN
GN - 3'
Tsp32ITaqITCGAT^CGA
AGC^T
5' - GC
Tsp32IITaqITCGAT^CGA
AGC^T
5' - GC
Tsp45IGTSAC^GTSAC
CASTG^
5' - CASTG
Tsp49IMaeIIACGTACGT^
^TGCA
ACGT - 3'
Tsp509ITspEIAATT^AATT
TTAA^
5' - TTAA
TspBIDsaICCRYGGC^CRYGG
GGRYC^C
5' - GRYC
Tsp4CIACNGTACN^GT
TG^NCA
N - 3'
TspDTIATGAAATGAANNNNNNNNNNN^
TACTTNNNNNNNNN^NN
NN - 3'
TspEIAATT^AATT
TTAA^
5' - TTAA
Tsp8EIBglIGCCNNNNNGGCGCCNNNN^NGGC
CGGN^NNNNCCG
NNN - 3'
TspGWIACGGAACGGANNNNNNNNNNN^
TGCCTNNNNNNNNN^NN
NN - 3'
TspRICASTGNNCASTGNN^
^GTSACNN
CASTGNN - 3'
Tth111IGACNNNGTCGACN^NNGTC
CTGNN^NCAG
5' - N
Tth111IICAARCACAARCANNNNNNNNNNN^
GTTRGTNNNNNNNNN^NN
NN - 3'
TthHB8ITaqITCGAT^CGA
AGC^T
5' - GC
TscAINNCASTGNNNNCASTGNN^
^NNGTSACNN
NNCASTGNN - 3'
TsoITARCCA(N)9NNTARCCA(N)9NNNNNNN^
ATRGGT(N)9NNNNN^NN
NN - 3'
TspMISma ICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
Uba4009IBamHIGGATCCG^GATCC
CCTAG^G
5' - CTAG
Uba153AIPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
UbaM39IPvuIICAGCTGCAG^CTG
GTC^GAC
Blunt
UnbIAsuIGGNCC^GGNCC
CCNGG^
5' - CCNGG
UthSISmaICCCGGGCC^CGGG
GGGC^CC
5' - GC
Uur960IFnu4HIGCNGCGC^NGC
CGN^CG
5' - N
Van91IPflMICCANNNNNTGGCCANNNN^NTGG
GGTN^NNNNACC
NNN - 3'
Vha464IAflIICTTAAGC^TTAAG
GAATT^C
5' - AATT
VneIApaLIGTGCACG^TGCAC
CACGT^G
5' - ACGT
VpaK32ISapIGCTCTTCGCTCTTCN^NNN
CGAGAAGNNNN^
5' - NNN
VpaK11AIAvaIIGGWCC^GGWCC
CCWGG^
5' - CCWGG
VpaK11BIAvaIIGGWCCG^GWCC
CCWG^G
5' - CWG
VspIATTAATAT^TAAT
TAAT^TA
5' - AT
XagIEcoNICCTNNNNNAGGCCTNN^NNNAGG
GGANNN^NNTCC
5' - N
XapIApoIRAATTYR^AATTY
RTTAA^Y
5' - TTAA
XbaITCTAGAT^CTAGA
AGATC^T
5' - GATC
XcaISnaIGTATACGTA^TAC
CAT^ATG
Blunt
XceINspIRCATGYRCATG^Y
R^GTACY
CATG - 3'
XciISalIGTCGACG^TCGAC
CAGCT^G
5' - AGCT
XcmICCANNNNNNNNNTGGCCANNNNN^NNNNTGG
GGTNNNN^NNNNNACC
N - 3'
XcyISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
XhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
XhoIIRGATCYR^GATCY
RCTAG^Y
5' - CTAG
XmaISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
XmaIIICGGCCGC^GGCCG
GCCGG^C
5' - CCGG
XmaCISmaICCCGGGC^CCGGG
GGGCC^C
5' - GGCC
XmaJIAvrIICCTAGGC^CTAGG
GGATC^C
5' - GATC
XmiIAccIGTMKACGT^MKAC
CAMK^TG
5' - MK
XmnIGAANNNNTTCGAANN^NNTTC
CTTNN^NNAAG
Blunt
XorIIPvuICGATCGCGAT^CG
GC^TAGC
AT - 3'
XpaIXhoICTCGAGC^TCGAG
GAGCT^C
5' - AGCT
XspIMaeICTAGC^TAG
GAT^C
5' - AT
YenIPstICTGCAGCTGCA^G
G^ACGTC
TGCA - 3'
ZanIEcoRIICCWGGCC^WGG
GGW^CC
5' - W
ZhoIClaIATCGATAT^CGAT
TAGC^TA
5' - GC
ZraIAatIIGACGTCGAC^GTC
CTG^CAG
Blunt
ZrmIScaIAGTACTAGT^ACT
TCA^TGA
Blunt
Zsp2IAvaIIIATGCATATGCA^T
T^ACGTA
TGCA - 3'

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