Protein Technical Resources
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| Protein Expression Basics | Protein Purification | Protein Tags |
| Cell Free Expression | Affinity Chromatography | GST-tagged Proteins |
| Bacterial Expression | Ion Exchange Chromatography | His-tagged Proteins |
| Yeast Expression | Gel Filtration Chromatography | MBP-tagged Proteins |
| Baculovirus Expression | Cell Lysis and Fractionation | Flag-tagged Proteins |
| Mammalian Expression | Protein Dialysis, Desalting, and Concentration | SUMO-tagged Proteins |
| Algae Expression | Native Protein Isolation | c-Myc-tagged Proteins |
| Comparison of Protein Expression Systems | Pulldown | Tag Removing and Protease |
| Chromatography | ||
| High-Performance Liquid Chromatography (HPLC) | ||
| Reversed-Phase Chromatography (RPC) | ||
| Hydrophobic Interaction Chromatography (HIC) | ||
| Chromatogram | ||
| Protein QC | Case Studies | |
| Protein Purity and Quantity Determination | Co-expression of Protein Complexes | |
| Endotoxin Level | *** expression in Bacterial System | |
| *** expression in Yeast System | ||
| *** expression in Baculovirus System | ||
| *** expression in Mammalian System |
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