Protein Technical Resources
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Protein Expression | Protein Purification | Protein Tags |
Cell Free Expression | Affinity Chromatography | GST-tagged Proteins |
Bacterial Expression | Ion Exchange Chromatography | His-tagged Proteins |
Yeast Expression | Gel Filtration Chromatography | MBP-tagged Proteins |
Baculovirus Expression | Cell Lysis and Fractionation | Flag-tagged Proteins |
Mammalian Expression | Protein Dialysis, Desalting, and Concentration | SUMO-tagged Proteins |
Algae Expression | Native Protein Isolation | c-Myc-tagged Proteins |
Pulldown | Tag Removing and Protease | |
Chromatography | ||
High-Performance Liquid Chromatography (HPLC) | ||
Reversed-Phase Chromatography (RPC) | ||
Hydrophobic Interaction Chromatography (HIC) | ||
Chromatogram | ||
Protein QC | Case Studies | |
Protein Purity and Quantity Determination | Co-expression of Protein Complexes | |
Endotoxin Level | *** expression in Bacterial System | |
*** expression in Yeast System | ||
*** expression in Baculovirus System | ||
*** expression in Mammalian System |
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