Protein Technical Resources

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Protein Expression Basics	Protein Purification	Protein Tags
Cell Free Expression	Affinity Chromatography	GST-tagged Proteins
Bacterial Expression	Ion Exchange Chromatography	His-tagged Proteins
Yeast Expression	Gel Filtration Chromatography	MBP-tagged Proteins
Baculovirus Expression	Cell Lysis and Fractionation	Flag-tagged Proteins
Mammalian Expression	Protein Dialysis, Desalting, and Concentration	SUMO-tagged Proteins
Algae Expression	Native Protein Isolation	c-Myc-tagged Proteins
Comparison of Protein Expression Systems	Pulldown	Tag Removing and Protease
	Chromatography
	High-Performance Liquid Chromatography (HPLC)
	Reversed-Phase Chromatography (RPC)
	Hydrophobic Interaction Chromatography (HIC)
	Chromatogram

Protein QC	Case Studies
Protein Purity and Quantity Determination	Co-expression of Protein Complexes
Quantitative Analysis	*** expression in Bacterial System
Endotoxin Level	*** expression in Yeast System
Protein Stability Testing	*** expression in Baculovirus System
Protein Aggregation Analysis	*** expression in Mammalian System
Protein Homogeneity

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