High-Pressure NMR Service

Inquiry

The combination of high-resolution NMR spectroscopy with pressure perturbation, called variable pressure NMR spectroscopy or high-pressure NMR spectroscopy, is a rapidly developing and promising technique for the future. The importance of this method lies in its ability to systematically detect and analyze the structure and thermodynamic stability of high-energy sub-states in proteins.

Figure 1. ¹³C-edited 1D ¹H NMR spectra of L391 Hδ1 recorded at pressures between 20 and 2500 bar. (Xu, X.; et al. 2021)

Creative Biostructure has high-end equipment with patented and proprietary design and manufacturing processes, modular design of various accessories for variable temperature, and high-pressure simulations. With high-precision thermostatic probes for more stable data acquisition, our NMR services provide systematic and unique information for a wide range of scientific disciplines.

Service Advantages

Large-bore NMR analysis and visualization system
Specialized variable temperature and high-pressure probes
Ring pressure tracking, pressure accuracy 0.25%
Providing information not only on the three-dimensional structure of biomolecules but also on their thermodynamic and kinetic properties, making it an essential tool for understanding the function of biomolecules at atomic resolution
Providing the possibility to monitor structural transformations that occur during protein unfolding at atomic resolution and determining the kinetic properties of the process
Equipped with modern cryogenic probes to provide unparalleled NMR performance and reliability

Practical Application Examples

Transition state measurements in enzyme catalysis.
Characterizing protein functional sub-states and protein structures.
Understanding the relationship between amino acid sequence, structure, and dynamic properties of the natural conformation of proteins.
Understanding the details of protein denaturation and partially folded state conformations and detecting intrinsic protein fluctuations.
Quantitative tests to analyze physical parameters such as pore structure, pore size distribution, and movable/bound fluid saturation in unconventional reservoirs.
Probing the thermodynamic and kinetic characteristics of proteins interconverting between stable folded conformations.

Creative Biostructure has high-end equipment to provide a full range of high-pressure NMR analysis services to customers in the fields of structural biology and molecular biophysics, helping our customers to obtain unique and in-depth research information. Please feel free to contact us to submit your analytical needs or feedback on our services, and you will receive a satisfactory response soon.

Ordering Process

Reference

Xu, X.; et al. Volume and compressibility differences between protein conformations revealed by high-pressure NMR. Biophysical Journal. 2021, 120(5): 924-935.