Single Particle Analysis (SPA)

In recent years, cryo-electron microscopy (cryo-EM) technology has made great progress, and it has been widely applied in the study of high-precision structures of biological macromolecules, especially in the structural analysis of protein complexes which are difficult to be solved by other approaches. Cryo-EM single-particle analysis (SPA) has become a popular and effective approach for studying the structure of biologically significant molecules at a near-atomic resolution. As a professional service provider in the field of structural biology, Creative Biostructure has extensive expertise and experience in SPA technology.

Advantages of Cryo-EM Single-Particle Analysis (SPA)

• Rapid freezing of the sample keeps the protein close to its natural state.
• Allows simultaneous analysis of diverse conformations of the target protein.
• Requires only a small amount of sample and is more tolerant to protein purity.
• Does not require the protein to crystalize.

The Workflow of Our Cryo-EM SPA Service

Our cryo-EM SPA service covers the entire technical process. The sample to be analyzed can be supplied by our customers or produced by us, and then subjected to negative staining EM (optional). Negative staining EM is a convenient approach for quickly screening homogeneous samples and offers an initial model for acquiring cryo-EM structures with high resolution. Followed by operations related to cryo-EM SPA, including sample vitrification, EM data acquisition and processing, three-dimensional (3D) model reconstruction and refinement.

Creative Biostructure offers comprehensive and unbundled cryo-EM SPA services, including but not limited to molecular cloning, protein expression, protein purification, cryo-EM SPA sample preparation, EM imaging, data processing and interpretation. Moreover, if you choose the cryo-EM SPA service, we can provide you with negative staining EM for free to screen out cryo-EM samples of high quality.

If your research project involves biological macromolecules or protein complexes that are difficult to crystallize, then our cryo-EM SPA service will effectively provide you with structural details about these molecules. Please feel free to contact us for more information or a detailed quote.

Ordering Process