In Vivo NMR Spectroscopy Service

In vivo NMR spectroscopy is a method that utilizes NMR technology to detect and analyze molecules within the body. It can provide detailed information about molecular structures and metabolic activities. Creative Biostructure has extensive experience in NMR projects and has provided NMR services to customers from around the globe.

What Is In Vivo NMR Spectroscopy Technology?

In Vivo NMR Spectroscopy is a non-invasive analytical technique that utilizes nuclear magnetic resonance (NMR) to study living organisms, including animals and humans. This technology allows for the observation of molecular structures, metabolic activities, and chemical changes within the body in real time. It is distinguished by its ability to provide detailed insights into biochemical processes without causing harm to the subject. It provides crucial data for various applications, including disease diagnosis, drug development, and biomedical research.

Figure 1. The basis of in vivo NMR spectroscopy. (Lysak D H, et al., 2023).

Why Perform In Vivo NMR Spectroscopy?

• Non-invasive: In vivo NMR spectroscopy allows for continuous, real-time monitoring of biological processes without the need for tissue sampling or other invasive procedures.
• High Resolution, Sensitivity, and Specificity: The technology is known for its high resolution, which allows for the differentiation of closely related compounds, high sensitivity to detect even minor changes, and high specificity to accurately identify the molecules of interest.
• Molecular and Metabolic Insights: It offers a way to study the molecular composition and metabolic pathways within organisms, which is crucial for understanding disease mechanisms and the effects of treatments.
• Disease Diagnosis and Drug Development: The data obtained from in vivo NMR spectroscopy can support the early diagnosis of diseases and the development of new drugs by providing insights into the biochemical changes associated with various conditions.
• Metabolomic Research: In vivo NMR spectroscopy has been extensively used in metabolomic studies, which involve the comprehensive analysis of small-molecule metabolites found within cells, biofluids, tissues, or organisms.
• Advantages Over Other Techniques: As a non-destructive and non-targeted method, NMR spectroscopy excels in accurately characterizing the chemical nature of biochemical processes and the organism's response to various stressors.

Our In Vivo NMR Spectroscopy Service

Creative Biostructure has a professional NMR service team with a profound background in nuclear magnetic resonance technology and extensive practical experience. We are committed to providing customers with high-quality and efficient NMR analysis services. Our one-stop NMR services include sample preparation, NMR data acquisition, data processing and analysis, and result interpretation. Every experimental step is strictly controlled and optimized to ensure the accuracy and reliability of the data. Here is an overview of our in vivo NMR spectroscopy workflow:

Figure 2. Our in vivo NMR spectroscopy workflow. (Creative Biostructure)

1. Project Initiation: The first step involves initiating the project with a clear understanding of the objectives and requirements. This stage sets the foundation for the entire process by defining the scope, goals, and specific needs of the in vivo NMR spectroscopy project.

2. Experimental Design: The experimental design is developed in this phase, which includes determining the parameters to be studied and the methods for data collection and analysis.

3. Sample Preparation: In this step, samples are collected from living organisms and prepared according to the project's needs, ensuring they are ready for the NMR data collection phase.

4. NMR Data Collection: This step focuses on the actual collection of NMR data, capturing the biochemical signatures of the samples. It involves obtaining relaxation, spectral, and other relevant data from the prepared sample.

5. Data Processing & Analysis: After the data collection, this step is crucial for processing and analyzing the raw NMR data. It includes noise reduction, spectral alignment, and quantification of metabolites to extract meaningful biological insights.

6. Project Summary: The project concludes with a comprehensive summary that encapsulates the study's objectives, methods, results, and conclusions. This summary serves as a report to stakeholders, detailing the project's outcomes and any implications for future research.

Creative Biostructure keeps up with the latest developments in international NMR technology and continuously explores new NMR application methods and technical means to promote the continuous progress and development of NMR technology. If you are interested in our in vivo NMR spectroscopy services, please contact us for a detailed quote.

Key Advantages of Our In Vivo NMR Spectroscopy Service

• One-Stop Service: From sample preparation to result interpretation, we provide a complete suite of NMR services.

  . Expert Team: Our team has extensive experience and deep expertise in nuclear magnetic resonance technology.

  . State-of-the-Art Equipment: We use the latest NMR technology for high-resolution and high-sensitivity data acquisition.

  . Commitment to Quality: Every step of the process is optimized and strictly controlled to ensure the highest standards of accuracy and reliability.

Frequently Asked Questions

• What types of organisms can your in vivo NMR spectroscopy service analyze?

  Our in vivo NMR spectroscopy service can analyze a wide range of organisms, including microorganisms, plants, invertebrates, and mammals (both small and large). Our technology is highly adaptable and can cater to the specific requirements of different biological subjects.

• What specific types of metabolic activities can your service analyze?

  Our service can analyze a broad spectrum of metabolic activities, including but not limited to, energy metabolism, amino acid metabolism, lipid metabolism, and nucleic acid metabolism. This helps in understanding various biochemical pathways and disease states within the organism.

• What are the typical turnaround times for the in vivo NMR spectroscopy service?

  The typical turnaround time varies depending on the complexity of the analysis and the volume of samples. However, we prioritize efficiency while ensuring meticulous work. We also offer expedited services for urgent projects.

Ordering Process

References

1. Valette J, Tiret B, Boumezbeur F. Experimental strategies for in vivo 13C NMR spectroscopy. Analytical Biochemistry. 2017. 529: 216-228.
2. Soong R, Liaghati Mobarhan Y, Tabatabaei M, et al. Flow-based in vivo NMR spectroscopy of small aquatic organisms. Magnetic Resonance in Chemistry. 2020. 58(5): 411-426.
3. Lysak D H, Downey K, Cahill L S, et al. In vivo NMR spectroscopy. Nature Reviews Methods Primers. 2023, 3(1): 91.