Application of Circular Dichroism Spectroscopy in Nucleic Acid Research

Circular dichroism (CD) spectroscopy technology is a fast, simple, and accurate method to study the secondary and tertiary structures of proteins, DNA, and energy biomacromolecules in solution using plane-polarized light. At present, it is widely used in studying the structure and function of these macromolecules. At Creative Biostructure, we offer a comprehensive suite of CD spectroscopy services tailored specifically for nucleic acid research. Our seasoned experts employ state-of-the-art instruments and advanced methodologies to provide you with accurate and detailed data, driving your research forward.

Significance of CD Spectroscopy in Nucleic Acid Studies

Nucleic acids, including DNA and RNA, lie at the heart of biological processes. Understanding their structure is key to deciphering their myriad functions. Understanding their conformational characteristics and interactions is crucial for unraveling the intricacies of biological processes. The circular dichroism spectrum of DNA is produced by the asymmetric sugar molecules in its backbone structure, and the helical structure determined by the configuration of these sugar molecules. Circular dichroism chromatography is a detection technique for studying the change of DNA conformation with changes in environmental conditions (temperature, ionic strength, and pH), and it is also a powerful tool for studying the interaction between DNA and ligands (including small molecules and large molecules such as proteins).

Figure 1. Circular dichroism application in DNA structure research. (Holmgaard List N, et al., 2017)

How to Study Nucleic Acid Using CD Spectroscopy

Our experts meticulously prepare samples, ensuring their purity and appropriate buffer conditions. The samples are then subjected to CD spectroscopy, and the resulting spectra offer a wealth of information. From the characteristic features of base pair stacking to the identification of helical conformations, CD spectra provide a comprehensive picture of the nucleic acid's structural landscape. We couple this data with advanced computational techniques to refine our understanding further, constructing detailed models that bridge the gap between sequence and structure.

What Can We Offer?

With rich CD spectroscopy service experience and excellent scientists, Creative Biostructure provides customers with the following circular dichroism spectroscopy applications in nucleic acid research:

• Nucleic Acid Secondary Structure Analysis
  With the high sensitivity to the secondary and tertiary structure changes of nucleic acid macromolecules, circular dichroism chromatography can be used to study the helical structure of DNA in solution.
• Monitoring DNA/RNA Structural Changes
  Leveraging the differential absorption of left and right circularly polarized light, our service discerns the intricate changes in chromophore environments, base stacking interactions, and helical conformations.
• Nucleic Acid Interaction Analysis
  By subjecting nucleic acid complexes to varying experimental conditions, we discern subtle changes in chiroptical signatures, elucidating the conformational alterations induced by interactions. Our service extends its prowess beyond qualitative analysis, employing quantitative methodologies to determine binding constants, stoichiometry, and thermodynamic parameters of these interactions.

Advantages of Our Service

• Expertise: Our team comprises seasoned researchers with extensive experience in structural biology, biophysics, and nucleic acid research.
• Cutting-Edge Technology: We stay at the forefront of technological advancements, utilizing state-of-the-art CD spectroscopy equipment. Our dedication to innovation ensures that you receive the most accurate and relevant data, driving your research forward.
• Comprehensive Insights: Our service not only furnishes you with raw data but also offers comprehensive analysis and interpretation, aiding you in making informed decisions.
• Tailored Solutions: We understand that each research question is unique. Thus, our service is customizable to your specific needs. Whether you're exploring DNA stability, RNA folding, or nucleic acid-ligand interactions, our approach is tailored to deliver results that matter to you.

Creative Biostructure offers comprehensive circular dichroism analysis to understand the structures and interactions of different nucleic acids. Embark on a journey of discovery with us as we harness the power of circular dichroism spectroscopy to unravel the secrets held within nucleic acids. Contact us today to explore how our expertise can catalyze your nucleic acid research endeavors.

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References

1. Miyahara T, Nakatsuji H, Sugiyama H. Helical structure and circular dichroism spectra of DNA: A theoretical study. The Journal of Physical Chemistry A. 2013, 117(1): 42-55.
2. Holmgaard List N, et al. Origin of DNA-induced circular dichroism in a minor-groove binder. Journal of the American Chemical Society. 2017, 139(42): 14947-14953.