Ultra Performance Liquid Chromatography Service

Creative Biostructure provides professional ultra performance liquid chromatography (UPLC) analysis services, dedicated to providing customers with high-sensitivity, high-resolution molecular separation and quantitative analysis. We have advanced equipment and an experienced technical team, which can meet the complex sample analysis needs in different fields and ensure accurate and reliable results.

What Is Ultra Performance Liquid Chromatography?

Ultra Performance Liquid Chromatography (UPLC) is a cutting-edge analytical technique that enhances traditional High-Performance Liquid Chromatography (HPLC) by leveraging smaller particle sizes, higher pressure, and improved detection capabilities. UPLC is widely recognized for its high resolution, rapid separation capabilities, and superior sensitivity, making it indispensable in modern analytical chemistry.

Key Advancements of UPLC over HPLC

• Speed & Efficiency: UPLC utilizes sub-2-micron particle size columns, significantly increasing separation efficiency and reducing analysis time.
• Higher Resolution: The smaller particle size allows better peak separation, enabling precise identification of closely related compounds.
• Enhanced Sensitivity: With lower detection limits, UPLC can analyze trace-level compounds with greater accuracy.
• Improved Throughput: Shorter analysis times and automation lead to increased sample processing efficiency.
• Lower Solvent Consumption: Reduced run times minimize solvent usage, making UPLC more cost-effective and environmentally friendly.
• High-Pressure Tolerance: UPLC operates at pressures exceeding 15,000 psi, which allows for greater chromatographic efficiency and better separation performance.

Due to these advantages, UPLC is a preferred method for biopharmaceutical analysis, environmental testing, material science, and food safety assessments. In addition, UPLC can also be used in conjunction with other detection methods such as mass spectrometry (MS) and fluorescence detectors to further enhance the ability of protein analysis.

Our Ultra Performance Liquid Chromatography Service

As a leading provider of structural biology and analytical solutions, Creative Biostructure offers UPLC services tailored to meet diverse research and industrial needs. Our state-of-the-art UPLC platforms, combined with expert analytical strategies, ensure precise molecular separation and quantification.

Service Workflow of Ultra Performance Liquid Chromatography

1. Sample Preparation: Optimizing sample extraction, purification, and concentration to ensure accurate and reproducible analysis.
2. Chromatographic Separation: Utilizing high-resolution UPLC columns and optimized mobile phase conditions for precise molecular separation.
3. Detection and Quantification: Employing advanced detectors such as mass spectrometry (MS) and fluorescence to enhance sensitivity and specificity.
4. Data Analysis and Interpretation: Providing comprehensive data processing, peak integration, and quantitative analysis to ensure reliable results.
5. Reporting and Consultation: Delivering detailed analytical reports with expert consultation to support further research and decision-making.

Application Areas

UPLC can be applied in many fields of science and technology due to its excellent separation efficiency, speed and sensitivity.

Figure 1. Application areas of ultra-high performance liquid chromatography. (Creative Biostructure)

Why Choose Creative Biostructure?

At Creative Biostructure, we deliver cutting-edge UPLC services with the following advantages:

• Unparalleled Precision & Sensitivity: High-resolution separation ensuring accurate quantification.
• Customized Analytical Strategies: Tailored protocols for complex biological, chemical, and material science samples.
• Integration with Advanced Detection Methods: LC-MS, UV, fluorescence, and refractive index detection for comprehensive molecular insights.
• Fast Turnaround Time: High-throughput capabilities for expedited sample processing and data delivery.
• Expert Technical Support: A dedicated team of chromatography specialists ensuring optimal analytical performance.

Whether you require small-molecule profiling, protein characterization, or impurity detection, our UPLC solutions provide precise, reproducible, and high-throughput results.

Frequently Asked Questions

• What are the key differences between UPLC and HPLC?

  UPLC offers higher resolution, greater sensitivity, and faster analysis compared to HPLC due to the use of sub-2-micron particle size columns and higher operating pressures (up to 15,000 psi).

• Can UPLC be used for large biomolecules such as proteins and polysaccharides?

  Yes, UPLC is highly effective for protein and polysaccharide analysis when combined with specialized columns (e.g., size-exclusion or hydrophilic interaction chromatography columns).

• What detection methods can be integrated with UPLC?

  UPLC can be coupled with Mass Spectrometry (MS), UV, fluorescence, and refractive index detectors, allowing for highly versatile analytical applications.

• How long does a typical UPLC analysis take?

  The duration depends on the sample complexity and analytical requirements, but UPLC typically reduces analysis time by 50% or more compared to HPLC. You can consult us for solution analysis.

Creative Biostructure provides high-quality UPLC services based on professional technical knowledge to meet the diverse needs of our customers. If you are interested in our services, please contact us for a detailed quote.

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