Surface Plasmon Resonance (SPR)
Real-time analysis of biomolecular interactions and binding kinetics
Structural Biology Services for Protein and Macromolecule Insights
Need to visualize how proteins and nucleic acids interact with their partners? Our comprehensive structural biology techniques map biomolecular interactions with atomic precision, providing high-resolution insights that bridge molecular structure and biological function. These services support therapeutic development, protein engineering, and fundamental research.
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Your Trusted Partner for Protein Structure Analysis – Membrane Protein as an Example
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Advanced Structural Biology Solutions Trusted by Leading Researchers
Creative Biostructure offers expert-driven, technology-focused, and cost-effective structural biology solutions to advance your research.
What Is Structural Biology?
Structural biology is a field of life science that focuses on determining and analyzing the three-dimensional structures of biological molecules, particularly proteins, nucleic acids, and their complexes at atomic resolution.
This cross-cutting combination of biochemistry, biophysics, and computer algorithms allows researchers to probe the way that structural changes shape molecular function.
Why Is Structural Biology Research Important?
Understanding molecular structures is crucial for drug discovery, protein engineering, and disease mechanism studies. It bridges the gap between sequence information and biological function, enabling rational design of therapeutics and biotechnology applications.
How Can You Support My Structural Biology Project?
Structural analysis meets a wide range of research and development needs. At Creative Biostructure, you can conduct any of the structural biology projects listed below or contact our specialists to design customized strategies that integrate multiple
technologies to tackle your unique research challenges. Our experts provide comprehensive support to help you achieve accurate results and interpret your data effectively.
Comprehensive Structural Biology Techniques for Your Research Success
At Creative Biostructure, we provide an extensive portfolio of structural biology technologies to meet diverse research needs. Our expert team guides clients in selecting the most suitable techniques based on specific research objectives, sample characteristics, and resolution requirements.
- Suitable for soluble proteins and well-ordered crystals
- Atomic resolution up to 1Å or better
- Excellent for protein-ligand complex structures
- Suitable for proteins of various sizes
- No crystallization required
- Suitable for membrane proteins or large molecular complexes
- Captures multiple conformational states
- Minimal sample amount needed
- Structure determination in solution state
- Real-time conformational analysis
- Protein dynamics investigation
- Direct observation of binding events
- Single-molecule visualization in native cellular environment without averaging
- Dynamic structural analysis in living cells
- Specific molecular identification via fluorescent labels
- Integration with other methods for in situ structural analysis
- Combines multiple techniques for complete structural insights
- Overcomes limitations of individual methods
- Captures dynamic states across different scales
- Enhanced reliability through cross-validation
Integrate Biophysical Assays to Enhance Structural Biology Research
Creative Biostructure offers multi-dimensional structural biology studies, integrating complementary technologies for deep insights into protein stability, conformational changes, and molecular recognition. Our expert team designs customized workflows combining biophysical methods to ensure reliable and comprehensive biomolecule characterization.
1. Binding Interaction Analysis
Isothermal Titration Calorimetry (ITC)
Direct measurement of binding thermodynamics and stoichiometry
Bio-Layer Interferometry (BLI)
Label-free detection of protein-protein and protein-ligand interactions
Microscale Thermophoresis (MST)
Solution-based measurement of molecular interactions with minimal sample consumption
Fluorescence Polarization (FP)
High-throughput screening of small molecule binding to proteins
2. Thermal Stability Analysis
Differential Scanning Calorimetry (DSC)
Quantitative analysis of protein thermal stability and folding characteristics
Thermal Shift Analysis (TSA)
High-throughput screening of protein stability and ligand binding
Isothermal Chemical Denaturation (ICD)
Assessment of protein conformational stability under various conditions
3. Spectroscopic Analysis
Circular Dichroism (CD) Spectroscopy
Rapid assessment of protein secondary structure and conformational changes
Fourier Transform Infrared Spectroscopy (FTIR)
Detailed analysis of protein secondary structure and aggregation states
Dynamic Light Scattering (DLS)
Size distribution analysis and aggregation monitoring of proteins in solution
Multi-Angle Light Scattering (MALS)
Absolute molecular weight determination and oligomeric state analysis
Raman Spectroscopy
Label-free characterization of local protein structure and ligand binding
4. Advanced Biophysical Methods
Analytical Ultracentrifugation (AUC)
High-resolution analysis of protein size, shape, and complex formation
Size Exclusion Chromatography (SEC)
Analysis of protein oligomeric state and complex formation
Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS)
Mapping protein dynamics and conformational changes with peptide-level resolution
Microscale Antibody Characterization (MAC)
Comprehensive characterization of antibody stability and functionality
Computational Analysis Services for Structural Biology Research
We leverage advanced computational methods and AI technologies to predict, model, and analyze biomolecular structures and their dynamics, providing valuable insights for structural biology research.
General and Quality Control:
- Structural Data Processing and Interpretation
- Structure Validation and Refinement
Structure Prediction:
- Homology Modeling
- Ab Initio Structure Prediction
- AI-Based Protein Structure Prediction
Dynamics Analysis:
- Molecular Dynamics (MD) Simulation
- Normal Mode Analysis (NMA)
- Principal Component Analysis (PCA)
Interaction Studies:
- Protein-Protein Docking
- Molecular Docking
- Free Energy Calculations
- Quantum Mechanics/Molecular Mechanics (QM/MM)
What Types of Biomolecule Structures Can We Help You Study
1. Protein Structure Analysis
- Single-Domain Proteins (Soluble Proteins, Membrane Proteins)
- Multi-Domain Proteins (Multi-subunit Complexes, Oligomeric Assemblies)
- Special Protein Types (Antibodies and Fragments, Enzymes, Receptors, Intrinsically Disordered Proteins)
2. Nucleic Acid Structure Analysis
- DNA Structure Characterization (DNA-protein Complexes, DNA Origami Structures)
- RNA Structure Characterization (Regulatory RNA Elements, Non-coding RNAs, RNA-protein Complexes)
- Nucleic Acid-Based Complexes (DNA-RNA Hybrids, Ribonucleoprotein Complexes)
3. Biomolecular Complex Analysis
- Protein-Protein Complexes (Stable Complexes, Transient Complexes)
- Protein-Ligand Complexes (Protein-Small Molecule Complexes, Protein-Peptide Complexes)
- Macromolecular Assemblies (Molecular Machines, Cellular Complexes)
4. Dynamic Structure Analysis
- Conformational States (Open/Closed States, Active/Inactive States)
- Structure Transitions (Allosteric Changes, Folding Intermediates)
- Time-Resolved Structures (Reaction Intermediates, Assembly/Disassembly States)
Our structural biology expertise covers comprehensive structure determination and characterization of diverse biomolecules, from single-domain proteins to complex macromolecular assemblies, including soluble and membrane proteins, antibodies, nucleic acids, and their dynamic interactions in both stable and transient complexes.
Various structures of proteins.
Advanced Structural Biology Platforms
We have developed structural biology platforms including X-ray crystallography platform, NMR spectroscopy platform, electron microscopy (EM) platform, and we employ a variety of cutting-edge instruments to support our extensive macromolecular structure determination capabilities. Our facilities are equipped with crystallization robots for automated crystal screening, high-throughput crystal imaging systems, 300 kV Titan Krios G3i, Bruker AVANCE 800 MHz Spectrometer, etc. These state-of-the-art facilities, combined with our experienced team, ensure reliable and precise structural analysis for your research projects.
Image Source: Hudson Robotics
Image Source: SPT Labtech
Image Source: Thermo Fisher Scientific
Image Source: Bruker
Featured Case Studies of Structural Analysis
Cryo-TEM for CDXX Receptor Structural Analysis
The goal of this project is to analyze the 3D structure of the CDXX and AB-X antibody complex using Cryo-TEM. Our work began with sample preparation, followed by data acquisition, image processing, and model building and refinement using single particle analysis (SPA) methods.
Figure 1. Representative image of CDXX-antibody complex (300 kV Cryo-TEM).
Figure 2. Representative 2D classification results.
Figure 3. The interaction between antibody CDR and CDXX.
Case Study
Cryo-TEM for CDXX Receptor Structural Analysis
Download this case study to explore the full details of Cryo-TEM analysis, including background, methodology, results, and key insights into the CDXX receptor structure.
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Why Choose Our Structural Biology Services
Our unique combination of integrated technologies, customized solutions, expert team, and comprehensive support provides unparalleled structural biology insights, accelerating your research from basic characterization to applications.
High-Resolution Structural Insights
Leverage advanced structural biology platforms, such as X-ray crystallography, NMR spectroscopy, and cryo-EM, for unparalleled insights.
Expert Structural Biology Team
Our team consists of highly experienced structural biologists, skilled in applying a range of technologies for precise biomolecular structure determination.
End-to-End Streamlined Services
From consultation and project design to execution and data interpretation, we offer seamless, efficient services tailored to your specific needs.
Customized & Cost-Effective Solutions
We deliver high-precision protein structure services with competitive pricing and strategies that meet the unique requirements of your research.
What Our Customers Say
Applications of Structural Biology in Advanced Research
Our structural biology services support drug discovery, protein engineering, and mechanism investigation, offering solutions for target structure analysis, protein-ligand interactions, structural-based drug design, and conformational dynamics studies.
Drug Discovery Support
- Target structure analysis
- Protein-ligand interaction research
- Structure-based drug design
- Lead compound optimization support
Protein Engineering
- Structure-based protein design
- Mutation effect analysis
- Stability enhancement research
Mechanism Investigation
- Molecular recognition analysis
- Binding site characterization
- Conformational dynamics research
- Structure-function relationship analysis
Structure-Based Drug Discovery
DownloadDownload our comprehensive brochure on structure-based drug discovery solutions to explore how our advanced techniques can accelerate your drug development process.
Research Publications Highlighting Our Structural Biology Expertise
Here are a few key publications highlighting the impact of our structural biology services:
| Article | Journal | Year | Description |
|---|---|---|---|
| APOE Christchurch-mimetic therapeutic antibody reduces APOE-mediated toxicity and tau phosphorylation | Alzheimer's & Dementia | 2024 | …We resolved the three-dimensional crystal structure of purified 7C11.IgGFab protein as a fee-for-service with Creative Biostructure … |
| A novel mechanism of herbicide action through disruption of pyrimidine biosynthesis | Proceedings of the National Academy of Sciences | 2023 | … Creative Biostructure (Shirley, NY) provided the X-ray crystallographic support … |
| Linoleic acid binds to SARS-CoV-2 RdRp and represses replication of seasonal human coronavirus OC43 | Scientific Reports | 2022 | ...It is a complex structure of SARS-CoV-2 RNA polymerase with double-strand RNA fragments (Creative Biostructure, Shirley, NY) … |
| Synthesis and biological evaluation of selective survivin inhibitors derived from the MX-106 hydroxyquinoline scaffold | European Journal of Medicinal Chemistry | 2021 | … We are currently working with a CRO (Creative Biostructure, Inc) to optimize the crystallization condition in order to obtain the crystal structures of survivin protein in complexes with this … |
| All major cholesterol-dependent cytolysins use glycans as cellular receptors | Science advances | 2020 | … We thank Creative Biostructure (NY, USA) for protein chemical shift assignment … |
FAQs About Our Structural Biology Services
How do you handle membrane protein samples for structural studies?
We utilize a systematic workflow combining initial stability screening, detergent/lipid optimization, and structure determination primarily through Cryo-EM or X-ray crystallography, with comprehensive validation using complementary biophysical methods. In addition, we are skilled in reconstituting membrane proteins into nanodiscs, virus-like particles, and liposomes for downstream studies.
What sample requirements are needed for Cryo-EM analysis?
For optimal Cryo-EM analysis, we typically require samples at 0.5-1 mg/mL concentration with >90% purity, demonstrated homogeneity by SEC and DLS, and a minimum molecular weight of approximately 100 kDa.
What computational methods do you use for structure-based drug design?
Our comprehensive computational pipeline integrates virtual screening, molecular dynamics simulations, free energy calculations, and machine learning approaches, all validated with experimental binding data from SPR/ITC measurements.
What is your typical timeline for protein structure determination projects?
Project timelines vary based on complexity, but generally we deliver initial structures within 2-3 months for well-behaved proteins using X-ray crystallography or Cryo-EM.
How do you handle protein complexes that are challenging to reconstruct due to weak or transient interactions?
We employ a multi-technique approach combining crosslinking mass spectrometry (XL-MS), small-angle X-ray scattering (SAXS), and high-resolution Cryo-EM with advanced computational modeling to characterize challenging protein complexes with weak or transient interactions.
If you have any questions, please contact our support team, who are dedicated to helping you maximize the potential of your research with Creative Biostructure.
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