Advanced Protein Production and Engineering Services

Struggling with challenging protein targets? Our comprehensive platform excels in membrane proteins and custom engineering, providing high-purity proteins, enhanced properties, and detailed characterization data to drive your research success with proven expertise.

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Protein production and engineering services.

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Schematic diagram of gene-directed protein synthesis.

The ability to produce and engineer proteins allows researchers to explore protein function in a controlled setting, as well as create novel proteins with optimized properties for drug discovery, industrial catalysis, and biotechnological applications. High-quality proteins are fundamental for studying protein-protein interactions, mapping epitopes, and determining 3D structures.

However, researchers encounter challenges including insufficient expression levels, protein misfolding, protein aggregation, and limited stability under experimental conditions. Moreover, the growing complexity of protein engineering projects, such as designing novel binding interfaces and optimizing catalytic properties, demands sophisticated technical approaches beyond conventional methods. Our comprehensive protein services platform addresses these challenges through advanced expression systems, innovative purification strategies, and rational protein engineering approaches.

Recombinant Protein Production

Our recombinant protein production services offer comprehensive and customizable solutions from gene to purified protein. We provide expertise across multiple expression systems such as E. coli, mammalian cells (CHO, HEK293), and insect cells (Sf9/Hi5), enabling optimal production strategies for diverse protein types. Our integrated workflow encompasses codon optimization, expression condition screening, and multi-step purification development. We deliver both research-grade proteins for basic research and structural studies, as well as GMP-grade materials for downstream applications. Each production process can be tailored to meet specific requirements for yield, purity, activity, and scale.

Expression System Selection
Expression Optimization
Protein Purification
Protein Characterization
Scale and Quality Options

Specific Solutions

Bacterial Protein Expression System (E. coli, Lactococcus lactis, Bacillus subtilis, Rhodobacter sphaeroides, etc.)
Mammalian Protein Expression System (CHO, HEK293, BHK-21, COS-7, etc.)
Insect Expression System (Sf9, Sf21 and Hi5)
Yeast Expression System (P. pastoris and S. cerevisiae)
Plant Cell Expression System
Cell-free Protein Expression System

Technical Details (Partial)

Strain screening and optimization
Vector design and construction
Expression validation
System-specific protocol development

Our Core Expertise — Membrane Protein Production

With specialized expertise in membrane protein production, we excel at expressing and purifying complex targets, including GPCRs, ion channels, and transporters, using optimized detergent screening and stabilization strategies. Our advanced technical platforms and extensive experience enable us to address various membrane protein challenges through a comprehensive suite of services—from cell-based and cell-free expression systems to both traditional detergent-based and innovative detergent-free methods. Our customizable solutions are tailored to ensure protein functionality and stability, meeting diverse research needs.

Service Category	Specific Solutions	Features	Applications
Protein Expression	Cell-based Membrane Protein Expression	Natural cellular environment for protein folding Suitable for post-translational modifications Well-established protocols Good for large-scale production	Complex membrane proteins requiring PTMs Projects requiring high yields Well-characterized protein targets
	Cell-free Membrane Protein Expression	Rapid expression Direct incorporation of labels/modifications No cytotoxicity concerns High flexibility in reaction conditions	Toxic proteins Quick screening studies Proteins requiring specific modifications Initial feasibility studies
	Animal-free Membrane Protein Expression	Eliminates ethical concerns Reduced risk of contamination Consistent batch-to-batch quality	Therapeutic protein development Applications requiring regulatory compliance
Protein Purification	Detergent-based Membrane Protein Purification	Well-established protocols High efficiency in membrane protein extraction Wide range of available detergents	Stable membrane proteins Structural studies High-throughput screening
	Detergent-free Membrane Protein Purification	Better protein stability Maintains native lipid environment Preserves protein-protein interactions	Sensitive membrane proteins Functional studies Complex protein assemblies
	Plasma Membrane Protein Isolation	Preserves native protein state Maintains associated protein complexes Good for studying protein interactions	Interaction studies Native complex analysis Proteomics applications
Protein Stabilization	Nanodiscs Construction	Defined size and composition High stability Suitable for various analytical methods Good for structural studies	Structural studies (Cryo-EM single-particle analysis, NMR) Functional studies
	Amphipol Reconstitution	High stability No free detergent Compatible with various techniques	Long-term stability Biophysical characterization Structural studies
	Liposome Reconstitution	Native-like lipid environment Suitable for functional studies Flexible composition	Transport studies Functional assays Drug delivery applications

Advanced Membrane Protein Products

Creative Biostructure offers comprehensive custom membrane protein production services, encompassing transmembrane protein production, monotypic/peripheral protein production, and membrane protein peptide production.

Leveraging the latest technologies, we also provide expertly assembled nanodiscs with embedded membrane proteins, ensuring enhanced protein stability and functionality in a native-like environment.

Nanodisc Human CD20/MS4A1 Protein Nanodisc Human CD133 Protein Nanodisc Human Claudin-18.2 Protein Nanodisc Human GPRC5D Protein Nanodisc Human Claudin-6/CLDN6 Protein

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Basic structure of assembled nanodiscs with embedded membrane proteins.

Schematic diagram of the structure of a liposome.

Additionally, we have developed a portfolio of ready-to-use membrane proteins in lipid vesicles via a cell-free expression system, offering clients reliable access to high-quality, advanced membrane protein products for diverse research applications.

CCR1 Liposome CCR2 Liposome CCR5 Liposome CXCR4 Liposome GLP1R Liposome

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Mastering Protein Engineering from Evolution to Design

Our protein engineering services seamlessly integrate with our protein production expertise, offering comprehensive solutions for protein optimization and modification. We combine rational design, directed evolution, and computational approaches to engineer proteins with enhanced properties – from improved stability and solubility to optimized activity and binding affinity. Our services span from targeted mutagenesis to complex protein evolution studies, enabling the development of proteins with tailored characteristics for research, industrial, and biotechnology applications. Whether complementing our protein production services or as standalone solutions, our engineering capabilities are backed by advanced computational tools, high-throughput screening platforms, and extensive expertise in protein structure-function relationships.

Service Category	Specific Solutions	Methods & Technologies (Partial)
Protein Evolution Services	Library Design & Construction Phylogenetic Analysis Directed Evolution Rational Design De novo Design	Computational design algorithms Machine learning-based design High-throughput screening platforms Sequence-structure relationship analysis Evolutionary trace methods Structure-guided protein design In silico modeling and simulation Automated library generation systems
Custom Mutagenesis Services	Site-directed Mutagenesis Libraries Site-saturation Mutagenesis Libraries Randomized Mutagenesis Libraries Scanning Point Mutation Libraries Degenerated Mutagenesis Libraries Truncation Libraries Combinatorial Assembly Libraries DNA Shuffling Custom Codon Optimization	PCR-based mutagenesis Golden Gate Assembly Gibson Assembly Overlap extension PCR Error-prone PCR NNK/NNS codon degeneracy Combinatorial design strategies Machine learning-guided optimization High-throughput DNA synthesis
Epitope Mapping Services	Linear Epitope Mapping Conformational Epitope Mapping Discontinuous Epitope Analysis Epitope Characterization Antigenic Determinant Analysis	Peptide array screening H/D exchange mass spectrometry Epitope mapping by 3D-EM Epitope mapping by NMR Epitope mapping by crystallography Surface plasmon resonance Computational epitope prediction Alanine scanning ELISA-based mapping
Advanced Characterization	Structural Analysis Binding Affinity Studies Stability Assessment Functional Validation Quality Control	CD spectroscopy Thermal shift assays SPR analysis BLI analysis Size-exclusion chromatography Mass spectrometry Dynamic light scattering Activity assays
Computational Analysis	Protein Structure Prediction Molecular Dynamics Simulation Protein-Protein Interaction Analysis Stability Prediction Function Prediction	AlphaFold integration Rosetta modeling Molecular dynamics packages Structure visualization tools Bioinformatics pipelines Energy calculation methods

Native Protein Isolation and Purification

Our native protein purification services leverage advanced isolation and purification technologies to extract proteins from diverse biological sources while maintaining their natural structure and function. We offer comprehensive solutions for purifying proteins from various sources including microbial cultures, cell lines, plant tissues, and biological materials. Our expertise spans from initial sample processing to final purification, ensuring high purity and biological activity for applications in research, industrial processes, and biotechnology innovations. With customizable protocols and rigorous quality control, we deliver native proteins suitable for various scientific and industrial applications.

Microbial Sources
Cellular Sources
Plant Sources
Biological Materials

Sample Types	Purification Solutions	Applications
Bacteria Yeasts Fermentation products	Cell disruption Fractionation Chromatographic separation Activity preservation	Enzyme production Antimicrobial research Industrial catalysts Bioprocess development

Sample Types	Purification Solutions	Applications
Cell lines Primary cells Tissue cultures	Gentle extraction Plasma membrane protein isolation Complex preservation Organelle separation	Protein-protein interaction studies Structural biology Functional studies Biotech applications

Sample Types	Purification Solutions	Applications
Plant tissues Seeds Leaves Roots	Tissue homogenization Green chemistry methods Bioactive preservation	Natural compounds research Enzyme studies Bioactive discovery Industrial applications

Sample Types	Purification Solutions	Applications
Various tissues Biological fluids Natural products	Biofluid processing Protein complex isolation Native state preservation	Basic research Industrial processes Biomarker studies Product development

Ready to Advance Your Protein Research?

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Contact our experts to discuss your protein challenges and receive a customized quote - whether you need standalone services or integrated solutions, we'll design the optimal strategy to drive your research forward.

Instrument Platform

Creative Biostructure is equipped with advanced instruments throughout the entire protein production and characterization workflow. For protein expression, we operate advanced bioreactor systems, automated cell culture platforms, continuous perfusion systems, and microfluidic cell culture devices. For protein purification and separation, we utilize ÄKTA protein purification systems with various chromatography options. Our analytical capabilities include high-resolution mass spectrometers (LC-MS/MS), multi-angle light scattering (MALS) detector, surface plasmon resonance (SPR) analyzer, bio-layer interferometry (BLI) system, and circular dichroism (CD) spectrometer. For protein characterization, we employ differential scanning calorimetry (DSC), dynamic light scattering (DLS) analyzer, and automated high-throughput screening platforms. Our structural biology studies are supported by cryo-electron microscopy (Cryo-EM), X-ray crystallography, and NMR spectroscopy facilities. Additionally, we maintain automated protein crystallization systems and robotic liquid handling workstations for high-throughput applications.

Multi-Angle Light Scattering (MALS) system.

Featured Case Studies of Protein Production Services

* NOTE: We prioritize confidentiality in our services to safeguard technology and intellectual property for enhanced future value and protection. The following case study has been shared with the client's consent.

Case #1
Case #2

Custom Protein Production and Nanodisc Assembly for F2 Receptor

The project aimed to produce and stabilize the F2 receptor membrane protein by assembling it within a Nanodisc. This method sought to improve the protein's solubility and structural stability, ensuring it remained functional and available for downstream applications.

In this case study, our innovative approach to optimizing membrane protein production and nanodisc assembly provided a robust solution for stabilizing the F2 receptor. By fine-tuning each step from expression to purification, we delivered a high-purity, stable protein-nanodisc complex tailored to the client's specific needs. This case demonstrates our ability to adapt protein production processes to meet the specific requirements of complex membrane proteins, providing clients with reliable, high-purity protein solutions for advanced applications.

Detection of F2 receptor in supernatant with varying Nanodiscs concentration gradient.

Detection of F2 receptor in precipitation across a Nanodiscs concentration gradient.

SDS-PAGE analysis showing the purified F2-nanodiscs complex.

Western blot analysis illustrating the purified F2-nanodiscs complex.

Case Study

Custom Protein Production and Nanodisc Assembly for F2 Receptor

This poster presents the complete case study content, including background, methods and materials, results, conclusions and discussion.

cDNA Optimization and Cloning
Nanodisc Assembly and Optimization
Purification and Quality Verification

Case Study on F2 Receptor Production and Nanodisc Assembly

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Why Choose Creative Biostructure?

Excellence in Protein Production
: With extensive expertise in both conventional and challenging proteins, we offer optimized solutions for prokaryotic, mammalian, and cell-free expression systems. Our strength in membrane protein production, supported by cutting-edge technologies, ensures exceptional protein quality and yield.
Advanced Protein Engineering Platform
: Our comprehensive protein engineering platform integrates directed evolution, rational design, and high-throughput screening capabilities. From custom mutagenesis libraries to sophisticated protein evolution strategies, we provide data-driven solutions for optimizing protein properties and functions.
Specialized in Challenging Targets
: We excel in handling challenging proteins, particularly membrane proteins and complex protein assemblies, through innovative approaches including advanced detergent screening, protein stabilization technologies, and detergent-free systems.
Customized Project Design
: Every project receives a tailored strategy based on specific requirements. Our flexible service model allows clients to choose from standalone services to integrated solutions, with adjustable scales from milligrams to grams.

"Our collaboration involved a complex membrane protein project. Their expertise in handling membrane proteins shone through, from tailor-made detergent selection to protein characterization. Their regular updates and adaptability to refine protocols based on preliminary outcomes were highly beneficial. The high-quality final protein product surpassed our expectations, facilitating our structural analysis. Their technical proficiency and problem-solving skills greatly hastened our research progress."

— Dr. Michael C., Senior Research Scientist, Institute of Molecular Biology

"Creative Biostructure enhanced our industrial enzyme's stability and activity through a blend of rational design and directed evolution. The high-throughput screening platform they custom-built for our project was highly effective. Their meticulous documentation and comprehensive data analysis clarified the optimization process. The resulting enzyme exhibited a fourfold stability boost and a 2.5-fold activity increase."

— Dr. Sarah A., Principal Investigator, Department of Biochemistry

"This team consistently delivers high-quality proteins, even with challenging targets. What sets them apart is their systematic approach and attention to detail. Using their automated platforms, they efficiently optimized expression conditions for multiple proteins simultaneously. Each batch comes with comprehensive analysis and documentation. Their technical expertise and consistent quality have made them our long-term partner for protein production."

— Prof. Allen S., Head of Protein Science

Protein Production and Engineering FAQs

What is the typical timeline for membrane protein production, from gene to purified protein?

For a typical membrane protein project, the complete process usually takes 6-8 weeks. This includes 2-3 weeks for expression system optimization and initial trials, followed by 3-4 weeks for purification optimization and final production. However, timeline can vary based on protein complexity and specific requirements. For challenging targets requiring extensive optimization, we'll provide a customized timeline during initial consultation.

How do you determine the most suitable expression system for my protein?

Our selection process is based on comprehensive analysis of protein characteristics, including size, modifications required, and complexity. We conduct parallel small-scale expression trials in multiple systems (E. coli, mammalian cells, insect cells) to evaluate expression level, protein quality, and functionality. This systematic approach allows us to quickly identify the optimal expression system for your specific protein.

What strategies do you employ when protein engineering projects face solubility or stability issues?

We implement an integrated approach combining computational analysis with experimental optimization. This typically involves structure-guided modifications, systematic mutagenesis, and high-throughput stability screening. Our advanced protein engineering platform enables rapid identification of optimal conditions and mutations. We continuously adjust our strategy based on real-time results to achieve the desired protein properties.

What quality control measures and documentation do you provide with the final protein?

Each protein batch comes with a comprehensive analysis package including purity assessment (SDS-PAGE, SEC), identity confirmation (Mass Spectrometry), homogeneity analysis (DLS), and project-specific functional assays. We provide a detailed production report containing all critical parameters and methods. Additional analyses can be included based on your specific requirements.

Can you handle both small-scale protein production for research and larger-scale production?

Our flexible production platform accommodates various scales, from milligram quantities for initial research to gram-scale production when needed. We develop optimized processes that maintain consistency across different scales, supported by our comprehensive monitoring systems. Our experienced team ensures smooth transition between scales while maintaining protein quality and functionality.

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