Custom TFF/Diafiltration Service
Creative Biostructure provides custom Tangential Flow Filtration (TFF)/Diafiltration service for advanced native/recombinant biological macromolecule isolation and purification programs.
Tangential Flow Filtration (TFF), also called cross flow filtration, is a powerful and rapid membrane filtration technique for biological macromolecules-protein, virus-protein, cell-protein, protein-salt separation and purification. It can be widely applied in various biological researches such as recombinant protein production, immunology, microbiology, biochemistry, etc. TFF performs the separation based on the different molecular size of biology molecules. TFF differs from the Direct Flow Filtration (DFF). In a TFF assay, samples are forced to run parallel to the TFF filter in a cycle, components with smaller size than the pores of TFF medium membranes will be pushed out through the pores. But components with larger size than the pores will have to flow parallel to the filter in the cycle. By this way, the separation is done according to the molecular size. A reservoir is usually used to improve the TFF assay so that the sample can be recycled through numerous times. TFF is also a wonderful technique for diafiltration and desalting. Various medium filters with different pore size have been developed to meet different experimental requirements.
Figure 1. Schematic diagram of a tangential cross flow filtration
The TFF process is simple and can be performed easily, TFF experimental system is very easy to set up and handle. With TFF, researchers can perform the higher concentrations with less time than dialysis. What's more, sample concentration and diafiltration can be performed on the same TFF system, saving the time and prevent the losses of sample. It is convenient for TFF to scaled-up or scaled-down, TFF can be applied to concentrate and desalt samples from 10ml to hundreds or even thousand liters with different TFF devices. The cost of a TFF assay is also low, as the devices and cassettes can be reused. The application of TFF is universal and can be involved in multiple experiments such as:
- Concentrate and desalt bio-macromolecules such as proteins, peptides, DNAs, RNAs, etc.
- Fractionate dilute protein mixtures
- Harvest cells.
- Deliver, recover and purify of certain macromolecules from cultured cells, blood or other sources.
- Prepare sample for chromatography.
- Recover or remove viruses.
- Clarify tissue homogenates or cell lysates.
Creative Biostructure now can provide custom TFF/Diafiltration Service for various biological researches. Various TFF systems are available for customers to meet your special requirements. Our science team will help you to establish the optimal TFF strategies for each individual case.
Creative Biostructure also provide other kind of custom chromatography technique services for biological macromolecule purification including but not limited to:
Affinity Chromatography
Ion-exchange Chromatography
Size-Exclusion Chromatography
Hydrophobic Interaction Chromatography
Reversed Phase Chromatography
Please feel free to contact us to discuss your project!
Ordering process
References:
- Christy C, Adams G, Kuriyel R, et al. High-performance tangential flow filtration: a highly selective membrane separation process[J]. Desalination, 2002, 144(1): 133-136.
- Benner R, Biddanda B, Black B, et al. Abundance, size distribution, and stable carbon and nitrogen isotopic compositions of marine organic matter isolated by tangential-flow ultrafiltration[J]. Marine Chemistry, 1997, 57(3): 243-263.
- Ugai H, Yamasaki T, Hirose M, et al. Purification of infectious adenovirus in two hours by ultracentrifugation and tangential flow filtration[J]. Biochemical and biophysical research communications, 2005, 331(4): 1053-1060.
