Transfersomes Production

Creative Biostructure offers liposome manufacturing services to elevate the current industrial standard with our cutting-edge technologies and approaches. Our experts are specialized in liposome manufacturing and characterization, and have already established the transfersome platform to transport drug through skin.

How Transfersomes Work

Different from other types of liposomes, transfersomes are mainly developed for transdermal drug delivery systems. Transfersomes are composed of self-assembled phospholipids (eg. phosphatidylcholine) and edge activators (such as a biocompatible surfactant or an amphiphilic drug) which increases lipid bilayer flexibility and permeability. Therefore, transfersomes can deform and pass through narrow constriction (from 5 to 10 times less than their own diameter) to underlying viable skin without measurable loss due to transdermal osmotic gradients which is developed by skin penetration barrier.

Transfersomes are an ideal choice for drug development of non-invasive therapeutic use. Based on the capability of increasing the transdermal flux, prolonging the release and improving the site specificity of bioactive molecules, transfersome has become a potential candidate for both systemic and topical delivery of drugs.

• Transfersomes have high entrapment efficiency (e.g. close to 90% for lipophilic drugs);
• Better penetration of intact vesicles due to its high deformability;
• A carrier for both low and high molecular weight drugs;
• Transfersomes protect the encapsulated drug from metabolic degradation;
• Easy to scale up, simple procedures do not involve unnecessary pharmaceutically unaccepted additives;
• Transfersomes are biocompatible and biodegradable as they consist of natural phospholipids.

However, chemical instability, phospholipid impurity, and expensive formulation limit the manufacturing of transfersomes. Creative Biostructure can overcome these obstacles to support the preparation using our professional knowledge and unique technologies.

Figure 1. Schematic presentation of mechanism of action of transfersome in transdermal delivery of anticancer agent. (Nano reviews & experiments, 2017)

Transfersomes Applications

Transfersome is a good carrier option to deliver drug into the skin layers for the treatment of skin cancers. Current application of transfersomes has been presented in treatment of actinic keratosis, basal cell carcinoma, squamous cell carcinoma, melanoma, and Kaposi’s sarcoma. In the future, it is likely that more transfersome applications will be developed based on its versatile encapsulating ability for proteins, anti-cancer drugs, anti-fungal drugs, analgesics, anesthetics, corticosteroids, sex hormone, insulin, albumin, herbal drugs, NSAIDS, interferons and more.

Creative Biostructure offers comprehensive services including transferosomes manufacture and analysis.

• Visualization: Performed using transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
• Particle size and size distribution: Determined by dynamic light scattering (DLS) and photon correlation spectroscopy (PCS).
• Entrapment efficiency: Measured by the ultracentrifugation technique.
• Vesicle stability: Determined by assessing the size and structure of the vesicles over time, and drug content can be quantified by high performance liquid chromatography (HPLC) or spectrophotometric methods.
• Penetration ability: Evaluated using fluorescence microscopy.
• In vitro drug release: Measured using a diffusion cell or a dialysis method.

Creative Biostructure persists in the best-quality service providing. Equipped with the latest expertise and advanced facilities, we can customize projects which completely meet your requirements. Please contact us for more information.

Ordering Process

References

1. Shubhra Rai, Vikas Pandey, Gopal Rai. (2017) Transfersomes as versatile and flexible nano-vesicular carriers in skin cancer therapy: the state of the art. Nano reviews & experiments. 8:1-18.
2. Reshmy Rajan, Shoma Jose et al. (2011) Transferosomes - A vesicular transdermal delivery system for enhanced drug permeation. Journal of Advanced Pharmaceutical Technology & Research. 2:1338-143.
3. Y. Dastagiri Reddy, A. B. Sravani et al. (2015) Transferosomes: a novel vesicular carrier for transdermal drug delivery system. Journal of Controlled Release. 2: 193-208.