Nano Differential Scanning Fluorimetry (NanoDSF)

Nano Differential Scanning Fluorimetry (NanoDSF) is an advanced, ultra-high-resolution differential scanning fluorescence technique that uses autofluorescence of tryptophan and tyrosine in proteins to detect their stability. It is widely used in the fields of antibody engineering, membrane protein research, formulation, and quality control.

Creative Biostructure uses nanoDSF technology to obtain de-folding curves with the ultra-high resolution based on unparalleled scanning speed and data point density for simple, fast and accurate analysis of protein folding and stability. The data is unaffected by sample aggregation and even instantaneous unfolding signals can be detected.

Figure 1. nanoDSF demonstrates the thermostabilization of RV-A2 by pleconaril. (Real-Hohn, A.; et, al. 2020)

Applications

• Monoclonal antibody formulation screening, drug screening, and target discovery.
• Identification of new ligands, affinity kinetic analysis of newly discovered protein drugs.
• Protein stability assessment, high throughput screening of protein stabilizers and inhibitors.
• Thermal stability parameters testing of protein binding to ligands, cofactors, and molecular chaperones.
• High-throughput screening of proteins for crystallization conditions.
• Screening of small molecule compound inhibitors.

Technical Advantages

• Low sample requirement (10 ul) and not limited by buffers.
• Wide range of assays for protein samples up to 250 mg/ml and down to 5 ug/ml. temperature range from 15 °C to 95 °C.
• No external dyes or sample processing is required, completely label-free. It can analyze the chemical and thermal stability of proteins by detecting changes in tryptophan and tyrosine autofluorescence only.
• Ultra-high resolution, with 48 capillaries tested every 3 seconds and over 1000 data points collected per sample.
• High throughput, reproducibility, and accuracy with simultaneous protein de-folding and aggregation detection.

Creative Biostructure offers nanoDSF technology services including chemical analysis of different protein families, identification of new ligands, and study of the stability of a large number of proteins in buffers with different chemical compositions. We have established an excellent platform for drug development and protein resolution to meet the diverse needs of our customers. You can contact us at any time to submit a nanoDSF analysis request.

Ordering Process

Reference

1. Real-Hohn, A.; et al. nanoDSF: in vitro label-free method to monitor picornavirus uncoating and test compounds affecting particle stability. Frontiers in Microbiology. 2020, 11, 1442.