Mass Spectrometry Imaging Service

Mass spectrometry imaging is a cutting-edge scientific research field derived from the development of mass spectrometry technology. It is a high-tech "photography" method that combines mass spectrometry technology and image visualization technology. It can analyze target molecules such as small molecules, peptides, and proteins without using specific labels. While providing structural information of target compounds, it can also reveal the spatial distribution and content changes of these molecules in the sample. Creative Biostructure is committed to providing high-quality mass spectrometry imaging solutions to meet the needs of scientific research and industry for high-precision molecular imaging.

The Significance of Mass Spectrometry Imaging

Through a high-resolution mass spectrometer, it is possible to directly obtain and draw a map of molecules such as proteins/peptides in a specific area. This not only helps to deeply understand the spatial distribution characteristics of molecules inside biological samples, but also helps researchers observe the changing laws of the target composition under different conditions (such as between disease state and healthy state). In addition, this technology is also of great significance for the study of drug metabolism dynamics. It can be used to track the distribution of drugs and their metabolites in the body, thereby providing valuable data support for the development of new drugs.

At present, there are three widely used and relatively mature mass spectrometry imaging technologies:

• Secondary ion mass spectrometry imaging (SIMS-MSI)
• Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI)
• Desorption electrospray ionization mass spectrometry imaging (DESI-MSI)

Creative Biostructure is committed to providing the above three advanced mass spectrometry imaging solutions to meet the needs of scientific research and industry for high-precision molecular imaging.

Figure 1. Workflow for different MSI methods. (PY Barre F, et al., 2017)

Our Service Procedure for Mass Spectrometry Imaging

Our MSI services cover a variety of target substances from small molecules to large molecules, including but not limited to:

At Creative Biostructure, we offer comprehensive MSI services tailored to a variety of research needs, from sample management to sophisticated data interpretation. Our end-to-end approach to MSI ensures accurate and exhaustive outcomes.

1. Sample Delivery: To maintain sample integrity, we require samples to be stored at -80°C and transported on dry ice. This ensures the stability of molecular structures prior to analysis.
2. Sample Preparation: Samples are meticulously prepared upon receipt, with tissue sections processed to align with the demands of the specific MSI method, optimizing imaging conditions.
3. MSI Procedure: Based on the research goals and sample characteristics, we select the most suitable MSI technique, such as SIMS-MSI, MALDI-MSI, or DESI-MSI. Each method is tailored to maximize the detection of target molecules within the sample.
4. Data Acquisition: High-resolution mass spectrometry data is collected during the experiment, following stringent, predefined parameters to ensure the precision of spatial and molecular information.
5. Data Analysis: Our sophisticated analysis involves thorough data mining and image creation, facilitating the extraction of key insights and a clear depiction of molecular distributions.
6. Results Compilation: We provide a comprehensive project report post-analysis, encompassing raw data and graphical representations. These reports offer a clear summary of our findings and actionable intelligence to advance your research goals.

Figure 2. Our MSI workflow. (Creative Biostructure)

Why Choose Creative Biostructure?

• State-of-the-art technology: Our lab is equipped with the cutting-edge mass spectrometry imaging systems for unparalleled measurement precision.
• Expertise: Our team boasts seasoned researchers with extensive experience, combining deep theoretical knowledge with hands-on expertise.
• Customized solutions: We are committed to customer-centric services, crafting bespoke solutions to meet specific needs.
• Comprehensive services: We offer an end-to-end service package, from solution design to data interpretation.

Frequently Asked Questions

• What types of samples can you process?

  We can process many types of biomedical samples, including animal tissues, plant materials, etc. Please let us know the specific sample type in advance so that we can make corresponding preparations.

• How do you ensure data security and privacy?

  All information provided by customers will be kept strictly confidential. No project details will be shared with the public unless expressly permitted. At the same time, we have also taken multiple security measures to protect electronic files stored on the server from unauthorized access.

• How do I submit a sample for analysis?

  Please contact us for detailed sample submission guidelines and to discuss your specific project requirements.

Creative Biostructure delivers premier MSI services, backed by expert technical insights to cater to diverse client requirements. If you are interested in our services, please contact us for a detailed quote.

Ordering Process

References

1. PY Barre F, MA Heeren R, Ogrinc Potocnik N. Mass spectrometry imaging in nanomedicine: unraveling the potential of MSI for the detection of nanoparticles in neuroscience. Current Pharmaceutical Design. 2017. 23(13): 1974-1984.
2. Chen K, Baluya D, Tosun M, et al. Imaging mass spectrometry: a new tool to assess molecular underpinnings of neurodegeneration. Metabolites. 2019. 9(7): 135.
3. Stoeckli M, Chaurand P, Hallahan D E, et al. Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nature Medicine. 2001. 7(4): 493-496.
4. Aichler M, Walch A. MALDI Imaging mass spectrometry: current frontiers and perspectives in pathology research and practice. Laboratory Investigation. 2015. 95(4): 422-431.