Exosome Spatial Omics Service
In organisms, cells must interact and combine in three-dimensional tissues. The position of each cell is as important as its inherent properties, determining the function of the tissue or the dysfunction of the disease. Therefore, even when studying the structure of simple organisms or single tissues, it is necessary not only to decipher the molecular profiles of thousands of cells, but also to understand how and what the spatial environment affects. Creative Biostructure provides advanced exosome spatial omics services to accurately locate and visualize the distribution of exosomes in tissues.
The Significance of Exosome Spatial Omics Analysis
Spatial omics, emerging after single-cell sequencing, addresses the spatial distribution of cells, which is a gap in single-cell sequencing. This technology examines cell positional relationships in tissues to understand their impact on diseases, focusing on proteins, mRNA, and their modifications. Despite its high costs and time-consuming nature, it offers fresh insights into areas such as animal development, brain structure, and tumor microenvironment characteristics linked to patient outcomes.
Exosomes, nanoscale vesicles secreted by cells, have been studied for their content and roles in physiological and pathological processes. They facilitate intercellular communication by carrying proteins and nucleic acids, crossing cell membranes, and influencing recipient cell functions. However, their distribution and effects on target cells are not well understood. By integrating spatial omics with exosome injections in experimental models, we can gain a deeper understanding of exosome behavior, shedding light on their potential in disease progression, therapeutic response, and drug delivery.
Figure 1. Spatial omics and multiplexed imaging. (Lewis S M, et al., 2021)
Our Comprehensive Service Portfolio for Exosome Spatial Omics
Creative Biostructure provides advanced exosome spatial omics services to accurately locate and visualize the distribution of exosomes in tissues. By combining spatial transcriptomics and spatial proteomics, we are able to reveal the interaction of exosomes with specific cell types and their impact on the local microenvironment.
Advanced Technologies
| Category | Technology | Specifications | Applications |
| Spatial Transcriptomics | smFISH |
|
|
| MERFISH |
|
|
|
| Imaging Systems | Super-resolution Microscopy |
|
|
| Light-sheet Microscopy |
|
|
|
| Mass Spectrometry | MALDI Imaging |
|
|
| Nano-HPLC-MS/MS |
|
|
Analytical Capabilities
| Analysis Type | Features | Outputs | Applications |
| Spatial Statistics |
|
|
|
| Machine Learning |
|
|
|
| Multi-Omics Integration |
|
|
|
| Data Visualization |
|
|
|
Our Workflow of Exosome Spatial Omics Service
- Project Planning and Design: Tailored experimental design incorporating client objectives, sample specifications, and analytical requirements to ensure optimal research outcomes.
- Exosome Extraction and Experiment: State-of-the-art exosome isolation and characterization of exosomes using ultracentrifugation, size-exclusion chromatography, and advanced validation techniques.
- Sample Collection and Processing: Standardized protocols for tissue preservation, sectioning, and preparation to maintain spatial integrity and molecular quality for downstream analysis.
- Spatial Omics Analysis: Integration of multiple spatial omics platforms to simultaneously analyze RNA transcripts, proteins, and other biomolecules while preserving spatial information.
- Data Analysis and Mapping: Advanced bioinformatics processing utilizing machine learning algorithms and statistical tools to generate comprehensive spatial-molecular maps.
- Project Report Compilation: Detailed documentation of methods, results, and interpretations, including high-resolution visualizations and statistical analyses in publication-ready format.
Figure 2. Our exosome spatial omics service workflow. (Creative Biostructure)
Why Choose Creative Biostructure?
- Rich project experience: many years of exosome research experience, with many successful cases.
- Multimodal analysis: combining transcriptome and proteome for comprehensive analysis.
- Professional data analysis: senior bioinformatics team, accurate interpretation of data.
- Customized service: tailor-made research plan according to customer needs.
Frequently Asked Questions
-
How is your data analysis capability?
Our senior bioinformatics team has extensive experience in data analysis and can efficiently process and interpret spatial omics data, providing one-stop services from raw data to advanced statistical analysis.
-
What types of samples are compatible with your service?
Our platform accommodates various sample types including fresh-frozen tissues, FFPE samples, and specially preserved tissue sections. Each sample type requires specific preparation protocols which our team will guide you through.
-
Can you analyze multiple tissue regions simultaneously?
Yes, our platform allows for multiplexed analysis of different tissue regions while maintaining spatial context and resolution.
Creative Biostructure offers premium services in the field of exosome research, leveraging our expertise to tailor solutions that align with your specific requirements. For more information or to obtain a comprehensive pricing breakdown, please contact us.
Ordering Process
References
- Lewis S M, Asselin-Labat M L, Nguyen Q, et al. Spatial omics and multiplexed imaging to explore cancer biology. Nature Methods. 2021. 18(9): 997-1012.
- Lin S, Zhao F, Wu Z, et al. Streamlining spatial omics data analysis with Pysod. Nature Protocols. 2024, 19(3): 831-895.
- Palla G, Spitzer H, Klein M, et al. Squidpy: a scalable framework for spatial omics analysis. Nature Methods. 2022. 19(2): 171-178.
- Xu C, Jin X, Wei S, et al. DeepST: identifying spatial domains in spatial transcriptomics by deep learning. Nucleic Acids Research. 2022. 50(22): e131-e131.
