Biological Science

Inquiry

Nuclear magnetic resonance spectroscopy (NMR) is an important spectroscopic research tool in molecular structure analysis. It can provide atomic-level molecular plane and three-dimensional structure, as well as dynamic information. With the continuous development of new technologies and methods, its research field and application scope has expanded to almost all fields of natural science.

As an expert in the field of nuclear magnetic resonance, Creative Biostructure has mastered mature NMR and related technologies, and can skillfully apply them to structural identification, conformational analysis, medical clinical detection, proteomics, metabonomics and other aspects.

Figure 1. Current applications of NMR in various fields of science. (Spiess, 2017)

Basic Principle of NMR

The chemical shift, coupling constant and relaxation rate of NMR spectrum essentially reflect the intrinsic structure and dynamics of matter. In particular, the relaxation rate contains the kinetic information from microsecond to millisecond within the molecule, and some important biological processes (such as protein folding) occur in this time scale.

Application of NMR in the Study of Protein Interaction

The interaction or combination between proteins and other biological molecules (such as DNA, RNA, polysaccharides, and drugs) is the main way to complete life activities. Because the nuclear magnetic resonance parameters (chemical shift, relaxation time, diffusion coefficient, etc.) of nuclear spin in binding and free ligand molecules are quite different, the kinetic information of protein structure change can be obtained through NMR, which is very useful for drug design and screening optimization. Moreover, NMR studies the kinetic properties of liquid protein in a close physiological environment, and the results obtained are more convincing. Our services include:

Determination of Protein Structure and Dynamics

Analysis of RNA Structure and Dynamics

Characterization of Protein-Ligand Interactions

Characterization of Protein-Protein Interactions

Characterization of Protein-RNA Interactions

NMR Mapping of Protein Interactions in Living Cells

Application of NMR in Chemical Structure Identification of Natural Products

Characterization of Dynamic Conformations of Oligosaccharides

Characterization of Large Biomolecular Complexes

Natural Product Analysis

Application of NMR in Biomembrane Dynamics

Nuclear magnetic resonance technology has become an important means to study membrane structure and membrane dynamics. It is well known that the bilayer membrane structure model has successfully explained the structure and function of the membrane, but the simulation and verification of the bilayer membrane structure have encountered great difficulties. The skilled application of nuclear magnetic technology provides an effective method to solve this problem. Our services include:

Analysis of Peptides in Oriented Biomembranes

Characterization of Lipid Membranes

Characterization of Peptide Structures and Peptide-Lipid Interactions in Membranes

NMR of Paramagnetic Compounds

Application of NMR in the Study of Metabolomics

By measuring and analyzing the urine, serum or other biological samples collected in turn within a certain time, we can get the dynamic change pattern of metabolites in organs. NMR can be used to carry out experiments under physiological conditions or close to physiological conditions within a certain temperature and buffer range without any physical separation (non-destructive, without damaging the structure and properties of the sample) and qualitative analysis of compounds, to characterize and study massive metabolic information and its change rules (metabolomics), thus obtaining rich biological information such as physiology, pathology, pharmacology, and toxicology. Our services include:

Metabolic Profiling for Molecular Complexity

NMR-based Metabolomics

Combining NMR and Mass Spectrometry for Metabolomics

Creative Biostructure is committed to providing high-quality NMR analysis services to advance the life sciences fields. If you have any questions or needs, please contact us and our customer service staff will help you the first time.

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