Exosome Analysis Services

Exosomes contain proteins, lipids, nucleic acids, and metabolites, it has become a topic of interest in medical research and molecular biology. With a unique molecular signature and it can be easily enriched from biofluids, which can provide very valuable information for early detection and disease monitoring. The composition and quantity of exosomes may be directly related to the pathological state of the patient, so the analysis of exosomes could be of great relevance for the diagnosis and prognosis of different cancers.

Exosomes in liver metastasis of colorectal cancer. Figure 1. Exosomes in liver metastasis of colorectal cancer. (Biting Zhou, et al. 2020)

Therefore, to acquire a deeper knowledge of exosome biology and accelerate the use of these extracellular vesicles in diagnostic tests or clinical research, Creative Biostructure provides sensitive, reproducible methods for exosome analysis.

Exosome RNA Sequencing Services

Nucleic acid is another important cargo that exosomes carry, including deoxynucleic acids (DNA), coding and non-coding ribonucleic acid (RNA) like messenger RNA (mRNA) and microRNA (miRNA). After exosome isolation, nucleic acids need to be accurately quantified in order to track many kinds of medical conditions and diseases, especially when nucleic acids of exosomes are greatly different from the parental cell content.

Creative Biostructure provides a complete exosome workflow solution to solve nucleic acids profiles of exosomes, starting from fast and efficient isolation of RNA to exosomal RNA sequencing using qRT-PCR and novel sequencing techniques.

Exosome Lipidomics & Metabolomics Services

Lipids, an important part of cellular physiology, are the major components of exosomes. The importance of lipids in exosome biology has been associated with the fact that the lipid content of exosomes differs from the parent cells and changes during physiological processes. Exosome lipidomics study provides great access to identify lipid-based biomarkers and unravel its monitoring function in diseases. Besides, a wide range of metabolites in exosomes could also be used for biomarker discovery by showing distinct metabolite populations during different disease treatment stages.

Our customized services provide comprehensive support for exosomal lipidomics & metabolomics study by using different analysis methods for data acquisition, analysis, and even interpretation: Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC), NMR spectroscopy, Mass spectrometry, etc.

Exosome Proteomics Services

Exosome proteomics has been recognized as easy access for biomarkers monitoring, providing information on the physiology study of the parent cells and exosomes. For analysis by mass spectrometry, exosomal proteins can be separated by SDS-PAGE, in-gel digested, followed by LC-MS/MS experiments. Based on our customer need, we can work from exosome isolation to data collection and analysis, a final report with results will be provided.

Creative Biostructure's exosome analysis services make it easy to profile exosome lipids, proteins and RNAs with our years of experience working on exosomes. Our expertise provides comprehensive support for your exosome study.

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References

  1. Biting Zhou et al. Application of exosomes as liquid biopsy in clinical diagnosis. Signal Transduct Target Ther. 2020, 5: 144.
  2. Sitar Simona et al. Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. Anal Chem. 2015, 87(18): 9225-9233.
  3. A comprehensive view of the characterization of exosomes. Sponsored by Wyatt Technology. 2018.
  4. Tatiana Altadill et al. Enabling metabolomics based biomarker discovery studies using molecular phenotyping of exosome-like vesicles. PLoS One. 2016, 11(3): e0151339.
  5. Alicia Llorente et al. Molecular lipidomics of exosomes released by PC-3 prostate cancer cells. Biochim Biophys Acta. 2013, 1831(7): 1302-1309.
  6. Kevin L Schey et al. Proteomics characterization of exosome cargo. Methods. 2015, 87: 75-82.
  7. Jeoffrey Schageman et al. The complete exosome workflow solution: from isolation to characterization of RNA cargo. Biomed Res Int. 2013, 2013: 253957.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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