How to Isolate Exosome?

Exosomes are membrane-secreted extracellular vesicles carrying nucleic acids, proteins, lipids, and metabolites that transfer cell-specific components to recipient cells and play a critical role in intercellular communication and signaling. In recent years, the role of exosomes in pathobiological processes has attracted much attention. It has been reported that exosomes have great potential as drug carriers, as biomarkers, and for developing therapeutic approaches.

Figure 1. Biogenesis of exosomes and other vesicles. (Chen J, et al., 2022)

What are Exosomes?

Exosomes are extracellular vesicles released by virtually all cell types and range in size from 30nm to 150nm. In addition to performing many biological functions (intercellular communication and signaling), several biological entities (e.g., proteins and microRNAs) in exosomes are closely associated with the pathogenesis of most human malignancies. Therefore, they may serve as valuable biomarkers for disease diagnosis, prognosis, and treatment.

With a great deal of research into the biological origin, material composition, and distribution in body fluids it has been found that exosomes have multiple functions. Research has found that exosomes play a critical role in the following processes:

Based on the great promise of exosomes as a tool for disease diagnosis and management, it is essential to isolate exosomes from various biological matrices efficiently, simply, and economically. Creative Biostructure has decades of experience in exosome research, and we have cutting-edge exosome isolation technology to provide clients with customized solutions for exosome preparation.

Our Exosome Isolation Technologies

At Creative Biostructure, we have developed and optimized methods and tools for rapid, scalable, and reproducible exosome isolation, providing high-throughput solutions for exosome heterogeneity and biomarker discovery.

Figure 2. Exosome isolation methods. (Sidhom K, et al., 2020)

Below is a summary of our exosome isolation methods.

What are the Applications of Exosomes?

• Diagnostic potential of exosomes

Exosomes are present in all biological fluids, based on the specificity and stability of their contents, making them useful as minimally invasive liquid biopsy tools.

• Therapeutic potential of exosomes

Exosomes are being actively explored as therapeutic agents either by themselves or as carriers for drug payload delivery. The engineering of exosomes further enhances their potential to intervene in a wide range of diseases.

• Exosome-based vaccine development

Exosomes derived from innate immune cells and tumor cells have the potential to be used as cancer vaccines. The use of exosomes to transfer membrane proteins or their contents to alter cancer cell phenotypes facilitates exosome-based vaccine development.

• Role of exosomes in regenerative medicine

Exosomes from mesenchymal stem cells are frequently used in regenerative and aesthetic medicine and can be utilized as a novel cell-free therapy for the treatment of skin diseases.

Order Our Exosome Products

In addition to exosome isolation services, Creative Biostructure also offers high-quality exosome kits. Standardized exosome isolation kits address the heterogeneity of exosomes and provide a reproducible, simple, and straightforward method of exosome preparation. Our kits make it easier for clients to isolate exosomes in the lab with comparable or better results than ultracentrifugation and without the need for specialized equipment.

Furthermore, our scientists have successfully developed exosome products from different sources that have multiple applications in exosome-based research, reflecting the functional status of their parental cells. Our products include exosomes isolated from cancer cell lines, exosomes isolated from stem cell lines, exosomes isolated from immune-related cell lines, exosomes isolated from general cell lines, exosomes isolated from body fluids, fluorescent exosomes/microvesicles, lyophilized microvesicles, exosomes isolated from human disease-state body fluids, and exosomes isolated from plant. Using our exosome products as a starting point for research helps clients contribute to the advancement of science and medicine.

Creative Biostructure has been following advances in exosome research for many years. With expertise in exosome isolation, characterization, analysis, engineering, and application development, we provide strong support to our clients. If you are looking for high-quality exosome products and reliable exosome isolation services, welcome to contact us anytime.

References

1. Chen J, et al. Review on Strategies and Technologies for Exosome Isolation and Purification. Front Bioeng Biotechnol. 2022. 9: 811971.
2. Sidhom K, et al. A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option? International Journal of Molecular Sciences. 2020. 21(18): 6466.
3. Gao J, et al. Recent developments in isolating methods for exosomes. Front Bioeng Biotechnol. 2023. 10: 1100892.
4. Li P, et al. Progress in Exosome Isolation Techniques. Theranostics. 2017. 7(3): 789-804.