HQExo™ Microvesicles-A549(LEV-11)
| Product Summary | |
|---|---|
| Name | HQExo™ Microvesicles-A549 |
Download Datasheet
|
|
| Cat No. | LEV-11 |
| Source | Microvesicles derived from human non-small cell lung cancer cell line (A549 cell line) |
| Product Overview | Microvesicles are a type of extracellular vesicles (EVs) that are derived by cell membrane blebbing with a diameter from 100 nm to 1000 nm. While exosomes are smaller with a diameter between 30-160 nm and released by cell exocytosis. Microvesicles involve in intercellular cross-talk and can transport molecules such as mRNA, miRNA, lipids and proteins between cells, which make microvesicle play an important role in disease diagnosis. Due to its molecular transfer function, circulating microvesicles may be useful for the delivery of drugs to specific target cells. HQExo™ microvesicles isolated from cancer cell lines could use as positive controls for ELISA, FACS, WB. It has been reported that microvesicle express CD40, selectins, integrins, and cytoskeletal proteins, and their membranes are highly enriched in cholesterol, phosphatidylserine, and diacylglycerol. Microvesicles/exosomes has attracted more and more attention to anti-cancer research and regeneration. Microvesicles can be purified by ultracentrifugation and precipitation, then characterized by nanoparticles tracking analysis (NTA) and ELISA or WB. Lyophilization is useful for a long-term storage at 4°C, and frozen liquid should be kept at -20°C to -80°C. Creative Biostructure standard microvesicles products guarantee higher purity and quality to meet our customer’s downstream analyses. |
| Form | Lyophilized powder. Reconstitute lyophilized exosome by adding deionized water for a desired final concentration. Centrifuge before opening to ensure exosomes are at bottom, resuspend exosomes by pipetting and/or vortex, please avoid bubbles. Centrifuge again and mix well for using. |
| Concentration | >1x10^9 particles |
| Storage | Lyophilized powder store at 4 °C. Resuspension store at -80°C. Recommended to avoid repeated freeze-and-thraw cycles. |
Online Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Frequently Asked Questions
-
Do quiescent cells secrete exosomes?
Yes, quiescent cells are known to secrete exosomes. Exosomes are small vesicles that are released by various types of cells, including both quiescent (non-dividing) and actively dividing cells. These vesicles are involved in cell-to-cell communication, as they carry a cargo of proteins, lipids, and nucleic acids that can be transferred to recipient cells.
-
What strategies can be employed to standardize mRNA expression within exosomes?
You can utilize miRNA genes like miRNA16, miRNA26a, miRNA221, miRNA22, miRNA181a, miRNA181c, miRNA103, miRNA191, let7d, as well as small RNAs (5SrRNA and U6snRNA) that are normally expressed in exosomes.
-
Do you offer items designed for conducting studies on tracking extracellular vesicles?
Creative Biostructure offers highly purified fluorescent exosome standards for tracking EVs in fluorescence microscopy experiments. These fluorescent exosomes are labeled with a green dye, ensuring stable fluorescent labeling. They are suitable for various applications, providing a long-lasting and well-visible signal.
