HQExo™ Exosome-CD63-EGFP(Exo-GC07)
| Product Summary | |
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| Name | HQExo™ Exosome-CD63-EGFP |
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| Cat No. | Exo-GC07 |
| Source | Exosome derived from human embryonic kidney cell line (HEK293, CD63-EGFP) |
| Product Overview | Exosomes are small extracellular vesicles with sizes of 30-160 nm, which is a subtype of extracellular vesicles (EVs). Exosomes are secreted by all cell types and play a crucial role in intercellular signaling and communication. Exosomes are nano-sized shuttles that transport signaling RNAs, lipids, peptides and proteins to other cells. Studying exosome contents to get an insight into their roles in disease initiation and progression. HQExo™ exosomes derived from human embryonic kidney cell line (HEK293) could use as positive controls for exosome isolation and functional research, such as ELISA, FACS, WB. Exosome can be purified by ultracentrifugation and characterized by nanoparticles tracking analysis (NTA) and ELISA or WB. Lyophilization is useful for a long-term storage at 4°C, and frozen liquid should be kept at -20°C to -80°C. Creative Biostructure standard exosome products guarantee higher purity and quality to meet our customer’s downstream analyses. |
| Form | Lyophilized powder/ frozen liquid |
| Concentration | >1x10^9 particles |
| Storage | Lyophilized powder store at 4 °C. Frozen liquid store at -20°C to -80°C. Recommended to avoid repeated freeze-and-thaw cycles. |
| Reconstitution | Reconstitute lyophilized exosome by adding deionized water for a desired final concentration. Centrifuge before opening to ensure exosomes are at bottom, resuspend exosomes by pipetting and/or vortex, please avoid bubbles. Centrifuge again and mix well for using. |
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Frequently Asked Questions
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Do quiescent cells secrete exosomes?
Yes, quiescent cells are known to secrete exosomes. Exosomes are small vesicles that are released by various types of cells, including both quiescent (non-dividing) and actively dividing cells. These vesicles are involved in cell-to-cell communication, as they carry a cargo of proteins, lipids, and nucleic acids that can be transferred to recipient cells.
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What strategies can be employed to standardize mRNA expression within exosomes?
You can utilize miRNA genes like miRNA16, miRNA26a, miRNA221, miRNA22, miRNA181a, miRNA181c, miRNA103, miRNA191, let7d, as well as small RNAs (5SrRNA and U6snRNA) that are normally expressed in exosomes.
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Do you offer items designed for conducting studies on tracking extracellular vesicles?
Creative Biostructure offers highly purified fluorescent exosome standards for tracking EVs in fluorescence microscopy experiments. These fluorescent exosomes are labeled with a green dye, ensuring stable fluorescent labeling. They are suitable for various applications, providing a long-lasting and well-visible signal.
