Mempro™ Immunoliposomes

Creative Biostructure has established an innovative Mempro™ Liposome platform to offer a wide range of customized products and services for both academic and industrial clients. In recent years, Creative Biostructure has applied Mempro™ Liposome Technology to develop novel functional liposomes, of which immunoliposomes are the most important ones.

Figure 1. Schemed diagram of PS-containing immunoliposomes which can escape from the immune system for a long time to optimize binding to a selected pathogen such as HIV-1. (R. A. Petazzi, et al., 2015)

Liposomes have been created to encapsulate a variety of biosensors to identify the analytes such as bacteria or proteins. Immunoliposomes are specially built to incorporate immunogens. Liposomes have shown a lot of advantages such as low toxicity, biodegradable and biocompatible, and they are easy to functionalize with high stability and efficiency by different formulations or modifications. Functional liposomes with specific ligands allow cell-specific delivery. The specific ligands vary from peptide to antibody fragment, and they are attached to the surface of liposomes to increase drug delivery and reduce random accumulation in nonspecific target tissues. Immunoliposomes are artificially prepared spherical vesicles composed of a lamellar phase lipid bilayer. Compared to traditional detection methods, novel methods by using immunoliposomes provide several diagnostic advantages by the tagged antibodies on their outer surfaces.

Immunoliposomes have been extensively applied in the treatment of a variety of diseases including Alzheimer’s disease. It is with particular difficulty to deliver drugs to the brain since the blood brain barrier presents. Therefore, numbers of drugs, which show great potentials for treatmemt of Alzheimer’s disease, are unable to reach the brain. To solve this problem, pegylated immunoliposomes which are targeted with anti-transferrin receptor and anti-Aβ mABs can be used because PEG is able to not only reduce the immunogenicity but also prolong the half-life time of liposomes.

Liposomes circulate for a period long enough in the blood stream as other nanoparticles, which usually cause the encapsulated drugs extensively accumulated in tumor tissue. This unique process is known as EPR (enhanced permeability and retention) effect, and it might because the tumor vasculature is leaky and disorganized. Liposomes do not support targeted delivery of drugs into a specific cell type, however, immunoliposomes have achieved targeted delivery upon the EPR effect by conjugating some mAB fragments to the surface of them. These mAb fragments allow immunoliposomes to bind and internalize into the target cell with high specificity.

The properties of liposomes can be quite different based on different liposome formulations. Creative Biostructure is specialized in developing and producing novel functional liposomes to meet your custom demands. Please feel free to contact us for a detailed quote.

References:
S. Shukala, et al. (2016). Immunoliposome-based immunomagnetic concentration and separation assay for rapid detection of Cronobacter sakazakii. Biosensors and Bioelectronics, 77: 986-994.
R. A. Petazzi, et al. (2015). Time-controlled phagocytosis of asymmetric liposomes: Application to phosphatidylserine immunoliposomes binding HIV-1 virus-like particles. Nanomedicine: Nanotechnology, Biology, and Medicine, 11: 1985-1992.
F. L. Tansy, et al. (2016). Potential of activatable FAP-targeting immunoliposomes in intraoperative imaging of spontaneous metastases. Biomaterials, 88: 70-82.