Mempro™ Virus-Like Particles (VLPs) Small Molecule Attachment Modification
Creative Biostructure has years of experience in the virus-like particles (VLPs) modification researches, our scientists have developed various methods for appropriate modification of VLPs. Our team provides custom Mempro™ VLPs small molecule attachment modification services.
VLPs are composed of viral structural proteins which are non-infectious protein structures and can self-assemble into either icosahedral or rod-like structures. VLPs can induce strong antibody responses duo to the repeated surface epitopes on them, and they are ideal for trafficking to the lymphatic system to cause T-cell responses, for they have a proper size, representatively 25-100 nm. In order to add new function to VLPs, a lot of studies have showed that their surfaces enable to be functionalized with fluorescent dyes, carbohydrates, polymers, peptides and other organic molecules. Scientists from Creative Biostructure are confident of performing high quality tailored small molecule attachment modification services of VLPs based on the advanced Mempro™ VLPs modification platform.
Figure 1. General strategy for the construction of multifunctional drug delivery vessels derived from genome-free (mtMS2) viral capsids. (Bioconjugate Chem. 2007)
- Externally Modification of VLPs Small Molecule Attachment
According to biomedical applications, spherical nanomaterials with differentiated surfaces probably can provide special attractive systems for the delivery of therapeutic agents. Creative biostructure has developed an ideal construct for these purposes which is composed of an externally modified surface displaying multiple copies of a ligand targeted specific cell receptors, as well as providing obstruction to antibody binding that would neutralize the carriers before reaching their destinations.
- Interior Modification of VLPs Small Molecule Attachment
In addition, there has another approach, the interior modification strategies, which is achieved by installing a high payload of imaging agents, drug cargo, or other molecules of biomedical interest in a modular fashion. The greatest advantage of this method is that internal functionality can expect to exert minimal effects on biodistribution. Researches have finished the dual surface covalent modification of the tobacco mosaic virus (TMV) with small molecules and polymers, besides, they also demonstrated the capacity of MS2 viral capsids to undergo a facile, site-selective dual surface modification to afford unique hybrid drug delivery vehicles.
Creative Biostructure has been pioneered in the VLPs production for a long time, we can offer various Mempro™ VLPs modification services. Please feel free to contact us for a detailed quote.
References:
Kovacs E W, Hooker J M, Romanini D W, et al. Dual-surface-modified bacteriophage MS2 as an ideal scaffold for a viral capsid-based drug delivery system[J]. Bioconjugate chemistry, 2007, 18(4): 1140-1147.
Schlick T L, Ding Z, Kovacs E W, et al. Dual-surface modification of the tobacco mosaic virus[J]. Journal of the American Chemical Society, 2005, 127(11): 3718-3723.
Douglas T, Young M. Host–guest encapsulation of materials by assembled virus protein cages[J]. Nature, 1998, 393(6681): 152-155.
Gupta S S, Kuzelka J, Singh P, et al. Accelerated bioorthogonal conjugation: a practical method for the ligation of diverse functional molecules to a polyvalent virus scaffold[J]. Bioconjugate chemistry, 2005, 16(6): 1572-1579.
