Custom MemPro™ T-cell Surface Glycoprotein CD3 Zeta Chain
Creative Biostructure provides custom MemPro™ gene-to-structure services for T-cell surface glycoprotein CD3 zeta chains. Our seasoned scientists can perform the production of membrane proteins and the determination/analysis of their three-dimensional (3D) structures (Figure 1).
Figure 1. 3D model of CD3 zeta chain.
T-cell surface glycoprotein CD3 zeta chain is a transmembrane protein belonging to the CD3 ζ (zeta)/FCER1G family, also known as T-cell receptor T3 zeta chain or CD247 (Cluster of Differentiation 247). This protein is part of a protein complex together with the T cell receptor (TCR), which can recognize various antigens with major histocompatibility complex (MHC) molecules. The structure of zeta transmembrane dimer consists of a left-handed coiled coil with polar contacts. Two aspartic acids are critical for dimerisation of zeta chain and assembly with TCR. The various signals that are triggered in T cells by antigen recognition are transduced not by the TCR but by invariant CD3 zeta that are noncovalently linked to the antigen receptor to from the T-cell receptor (TCR) complex. TCR and CD3 complex includes a TCR alpha/beta (or T) heterodimer and the invariant subunits of CD3 labelled gamma, delta, epsilon, zeta, etc. at the cell surface. The CD3 zeta chain consists of copies of a sequence motif named Immuno-receptor tyrosine-based activation motifs (ITAMs) in intracellular domain, playing a critical role in intracellular signal-transduction after antigen recognition (Figure 2). As shown in Figure 2, the ability of the CD3 zeta chain to deliver intercellular signals is its interactions with protein tyrosine kinases (PTKs). Src-like PTK, called Lck, is responsible for binding to the cytoplasmic domains of CD4/CD8 and triggering the phosphorylation of tyrosine residues in the CD3 zeta chain of the TCR complex. The gene encoded CD3 zeta chain located on chromosome 1, is 87,896 bases in length. The CD3 zeta chain is 164 amino acids long with a predicted weight of 18.696 kD. It has been shown that T-cell surface glycoprotein CD3 zeta chain can interact with Janus kinase 3 and Protein unc-119 homolog.
Figure 2. Signaling transduction by CD3 zeta chain.
Creative Biostructure has successfully conducted functional and structural analysis for hundreds of membrane proteins. We can offer you sophisticated strategies to meet your specific needs. Creative Biostructure can provide custom MemPro™ membrane protein gene-to-structure services. Please feel free to contact us for a detailed quote.
References:
CD247. (https://en.wikipedia.org/wiki/CD247).
T-cell receptor. (http://slideplayer.com/slide/5310658/).
T-cell surface glycoprotein CD3 zeta/eta subunit/High affinity IgE receptor gamma subunit (IPR021663). (http://www.ebi.ac.uk/interpro/entry/IPR021663).
