Mempro™ Surface Plasmon Resonance (SPR) Technology

Based on the advanced and comprehensive Mempro™ nanodisc analysis platform established for years, Creative Biostructure offers high-quality surface plasmon resonance (SPR) technology service for nanodisc production.

SPR technology has quickly turned into a standard technique in the biotechnology and pharmaceutical industries, because of its excellent characteristics that enable the identification of the interactions of almost any molecular system, including nanodiscs. This technique is broadly applied to research protein–ligand interactions taking place in a thin fluid layer directly bind to a gold-coated biosensor chip, which constitutes the heart of microfluidic flow cells. The ligand, one of the interaction partners, is required to be settled at the biosensor surface. While the analyte, the other partner, is transfused into the cell at a variety of concentrations and flows across the biosensor. SPR measures the variation in refractive index an ultra thin boundary layer, which is immediately proportional to a quantity of protein in this region, that is, immobilized ligand and bound analyte.

Figure 1. The schematic of (a) grating-, (b) prism- (c) waveguide-based coupling configurations for SPR generation and (d) the surface plasmon excitation possibilities by using evanescent wave in the dispersion curves. (Gupta, B. D. 2016)

• Nanodiscs Analysis by Surface Plasmon Resonance (SPR)
  Nanodiscs are soluble nanoscale phospholipid bilayers, which are encircled and stabilized by membrane scaffold proteins (MSP), and they have been proven to be powerful and promising tools to study membrane proteins. The hydrophobic edge of the lipid bilayer is airproofed with the recombinant MSPs. A number of membrane proteins have been successfully reconstituted into nanodiscs, thus making selection and independent characterization of oligomeric states of membrane proteins available. Mercantile possible sensor chips with a variety of surfaces particularly designed for immobilization of membranes and membrane proteins have appeared and rapidly developed.
• Advantages of Surface Plasmon Resonance (SPR)
  By reconstitution into nanodiscs, membrane protein can be used as analyte molecular interaction studies using standard SPR-based methodology. Most of SPR-based interaction researches have been accomplished with soluble ligand and analyte molecules. In the last few years, reports on SPR researches on membrane proteins have been constantly increased. There are many advantages of SPR technique, for example, it allows label-free determination, interactions enable to be detected in real time, as well as just light ligand and analyte are needed for the determination of kinetic rates and equilibrium constants of protein interactions.

Creative Biostructure has been focusing on nanodisc technology for a long time, our team is confident in providing unparalleled nanodisc analysis service using SPR technology. Creative Biostructure also provides other various Mempro™ nanodisc technology services , besides nanodiscs analysis. Please feel free to contact us for a detailed quote.

References:
Glück, J. M. (2011). “Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies”. Analytical biochemistry, 408(1), 46-52.
Gupta, B. D. (2016). “Surface Plasmon Resonance-Based Fiber Optic Sensors Utilizing Molecular Imprinting”. Sensors 16(9), 1381.
Debu, D. T. (2016). “Effect of Ti adhesive layer on individual gold nanodisk surface plasmon resonance”. In APS Meeting Abstracts.