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Lipid Droplets Biofuel Supply

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As an established expert in artificial liposomes manufacturing, Creative Biostructure continues the effort in extending the customer population and developed a platform that is focused on transforming natural lipid droplets into biofuels. We provide substantial support to in vitro functional studies based on our advanced techniques and skills.

Why use lipid droplets as biofuel?

Nowadays energy generation heavily relies on fossil fuels causing environmental challenges. The global biofuels supply has increased by a factor of 8% since 2010, but only comprises 4% of the world’s transport fuels in 2015. The development of next generation of biofuel becomes increasingly important due to the depletion of fossil fuels and in the meantime to overcome challenges for current biofuels production – high cost and low efficiency. The biological production of lipid droplets in oleaginous microorganisms like microalgae, yeast, fungi, and bacteria becomes a promising path to the next generation of biofuels.

The lipid droplet (LD) is a cellular organelle that consists of a neutral lipid, mainly of triacylglycerols (TAGs) and cholesteryl esters, cored with a monolayer-phospholipid membrane and associated proteins. Lipid droplets widely exist in both prokaryotic and eukaryotic cells, could be collected and extracted for biofuel manufacturing. However, this technology is now limited in lab research. Methods to improve the lipid droplet production in oleaginous microorganisms, biomass pretreatment, lipid droplet extraction, industrial scalability are still under development. The experience of liposome manufacturing provides us a solid ground for lipid droplet studies and helps our clients move to a further step of new biofuel development.

Two types of lipid droplet in plants, oleosin-based lipid droplets (OLDs) & nonoleosin-based lipid droplets Figure 1. Two types of lipid droplet in plants, oleosin-based lipid droplets (OLDs) & nonoleosin-based lipid droplets (NOLDs).

(Journal of Biotechnology, 2015)

Creative Biostructure offers professional services for organelles studies:

  • Lipid droplet content analysis.
  • Lipid droplet production elevation: LD proteins characterization, culture condition optimization, microorganism selection, etc.
  • Biomass pretreatment method development.
  • Lipid droplet extraction assay development.
  • Industrial scale lipid droplet production and extraction.

With the most advanced technologies in liposome manufacturing, Creative Biostructure can support all types of research on artificial lipid membranes. Please contact us to share more details, we will certainly customize suitable plan to meet your satisfaction.

Ordering process

Ordering Process

References

  1. Yang Wang, Xiao-Ming Zhou, Xuejing Ma et al. (2016) Construction of nano-droplet/adiposome and artificial lipid droplets. ACS Nano. 10: 3312-3322.
  2. Christina G. Siontorou, Georgia-Paraskevi Nikoleli, Dimitrios P. Nikolelis. (2017) Artificial lipid membranes: past, present, and future. Membranes (Basel). 7(3): 38.
  3. Natalie Laibach, Janina Post, Richard M. Twyman et al. (2015) The characteristics and potential applications of structural lipid droplet proteins in plants. Journal of Biotechnology. 201: 15–27.
  4. Kathleen Araújo, Devinder Mahajan, Ryan Kerr, Marcelo da Silva. (2017) Global biofuels at the crossroads: an overview of technical, policy, and investment complexities in the sustainability of biofuel development. Agriculture 2017, 7(4), 32.

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