Mempro™ Acyl CoA Binding Protein (ACBP) Production Using Virus-Like Particles

Creative Biostructure now can provide custom Mempro™ Acyl CoA binding protein (ACBP) production service using virus-like particles.

The Acyl CoA binding protein (ACBP) is a small protein that consists of 86-103 amino acid residues and the amino acid sequences of ACBPs are highly conserved. ACBPs can bind with long-chain Acyl-CoA esters (LCA) with high affinity to regulate the function of LCA, and also plays an important role in intracellular Acyl-CoA transport and pool formation. ACBPs have been identified from various species including yeasts, plants, humans and reptiles, and can be categorized into at least four groups based on sequence alignment. The first group is 86-92 amino acid residues long and is the basic expressed ACBP which contains no Cys. The second group contains three Cys identified as testis specific isoform (t-ACBP), also called endozepine-like protein (ELP). The third group contains only one Cys at the position 43, named as brain specific isoform of ACBP (b-ACBP) as they are obtained from brains of some certain species like duck and frog. The fourth group, however, is the long sequence ACBP group that contains up to 533 amino acid residues. Some of these group members are reported to be membrane-bound ACBP domain proteins. Many of these long sequence ACBPs contain one or more Cys residues.

Figure 1. The schematic structure of human Acyl-CoA-binding domain-containing protein 4. (OPM Database)

Virus-like particles (VLPs) are self-assembled "viral empty shells" formed by viral structural proteins. VLPs act the same as native virus in many ways, except that all VLPs are non-infectious as they lack of viral genomes. VLPs have been used in various biological researches such as vaccine development, antigen delivery, and recombinant membrane protein production. Many viral tools have been developed to produce VLPs from multiple virus families, including but not limited to Parvoviridae, Flaviviridae, Retroviridae, etc.

Creative Biostructure now can provide high-quality custom Mempro™ ACBP production service with our most advanced VLPs technique. Various host systems are available to meet different experimental requirement, these host systems including bacteria, yeasts, green plants, insect cells, mammalian cells. We will co-express the viral core structural protein along with the target ACBP proteins in host cells. The expressed core structural protein will perform the self-assembly within plasmid membrane of host cells, and budding-off from the plasmid membrane to form lipoparticles(VLPs that contain high concentration of specific membrane proteins within their construction). The expressed target ACBP protein will be captured by lipoparticles during the same time. We will obtain the VLPs from the surface of host cells. The whole program is free from detergents and mechanical disruptions, and the target ACBPs are protected within the lipoparticles so that their orientation of membrane proteins and structure remains native and complete.

With years of experience in membrane protein production, Creative Biostructure can also provide other wide ranges of Mempro™ membrane protein production using advanced VLPs technique. Welcome to contact us for more details.

References:
Knudsen J, Neergaard T B F, Gaigg B, et al. Role of acyl-CoA binding protein in acyl-CoA metabolism and acyl-CoA–mediated cell signaling[J]. The Journal of nutrition, 2000, 130(2): 294S-298S.
A.Roldão, et al. Virus-like particles in vaccine development.Expert Rev. Vaccines, 2010,9(10): 1149-1176.