Mempro™ Plant-Based Phosphotyrosine Protein Phosphatases II Production

Creative Biostructure can provide world-leading custom phosphotyrosine protein phosphatases (PTPs) II production services using plant-based expression system. Our plant-based expression system has lots of advantages for membrane protein production, such as high-yield, animal-free, low protease activity, and low endotoxin, etc.

Creative Biostructure is highly-experienced in providing excellent membrane protein production services using various types of plant hosts, including Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce. We can perform two different strategies for membrane protein production in plants: transient expression and stable transformation. We can produce animal-free recombinant membrane proteins, which are very important for customers concerned with experimental variables caused by trace animal components or mammalian pathogens. Recombinant membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. Additionally, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system.

Figure 1. Structural model of protein tyrosine phosphatase 1. (Wikipedia)

Phosphotyrosine protein phosphatase (PTP) II superfamily is composed of five membrane protein families: the mycobacterial PtpB-like family, the myotubularin-like phosphatases family, the higher-molecular-weight phosphotyrosine protein phosphatases family, the myo-inositol hexaphosphate phosphohydrolase (phytase) PhyA family and the dual specificity phosphatase-like family. Protein Try phosphorylation is an important post-translational modification (PTM) motif that provides a novel recognizing motif for protein interactions and cellular localisation. Through this way, members from PTPs II superfamily play a crucial role in many cellular processes, including cellular differentiation, cell growth, signal transduction, etc. Coupled with tyrosine kinases, PTPs control the phosphorylation state of biological molecules.

With the Mempro™ plant-based protein production system, Creative Biostructure is able to express, isolate and purify phosphotyrosine protein phosphatases (PTPs) II superfamily to facilitate the study of their biological functions. We can overcome the common difficulties encountered in cell-based expression system, such as misfolding, aggregation, inactivity, high endotoxin, poor stability and solubility, etc.

Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
A. Caselli, et al. (1995) In vivo inactivation of phosphotyrosine protein phosphatases by nitric oxide. FEBS Letters, 374(2): 249-252.
A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.
Protein tyrosine phosphatase. (https://en.wikipedia.org/wiki/Protein_tyrosine_phosphatase)