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Mouse MSP1E3D1 lyophilized protein with a his-tag(MSP-1006)

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Product Summary
Name Mouse MSP1E3D1 lyophilized protein with a his-tag
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Cat No.MSP-1006
ShortnameMSP1E3D1
SourceE.coli
Product OverviewRecombinant Mouse membrane scaffold protein 1E3D1 was expressed in E.coli with N-terminal His tag.
Purity>90%
SpeciesMouse
TagHis
Molecular Mass32.53kDa
StorageStored lyophilized powder at -20°C. The reconstituted solution should be stored at 2-8°C and should be used up in several days.
BackgroundNanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein coat that confers enhanced stability and a narrow particle size distribution. The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.
ReconstitutionAdding double distilled watwe to prepare a stock solution of 4mg/mL. This stock solution can be diluted further as required by the differnet application protocols.
Scaffold Diameter12-14 nm
FormulationLyophilized from 20mM Tris pH 7.4, 100 mM NaCl, 0.5 mM EDTA.

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Frequently Asked Questions Frequently Asked Questions

  • What are the key advantages of pre-assembled nanodiscs?

    Pre-assembled nanodisc products offer a streamlined solution for membrane protein studies. They provide a ready-to-use platform, reducing preparation time and ensuring consistent results.

  • Can you elaborate on the compatibility of pre-assembled nanodiscs with common analytical techniques?

    Pre-assembled nanodiscs are compatible with a range of analytical techniques, including NMR spectroscopy, circular dichroism, and various chromatographic methods. Their well-defined structure facilitates accurate analysis of membrane protein properties, allowing researchers to obtain detailed insights into structure, dynamics, and interactions.

  • What quality control measures are in place for pre-assembled nanodiscs?

    Quality control measures for pre-assembled nanodiscs typically include rigorous testing of stability, homogeneity, and functionality. We can provide detailed QC data with each product batch.

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