Mempro™ Plant-Based Peptidase MA Production

Based on the comprehensive membrane protein production platform established through years of experience, scientists from Creative Biostructure can provide customized Mempro™ peptidase MA production services using plant-based expression system.

Creative Biostructure has rich professional experience in high-yield, animal-free, low endotoxin, and low protease activity peptidase MA using Mempro™ plant-based expression system. Animal-free recombinant membrane proteins are particularly important for customers concerned with experimental variables caused by trace animal components or mammalian pathogens. Notably, membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination.

We can provide plant-based membrane protein production services using various types of host plants such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce. we are capable of performing two different strategies to fulfill your specific demands: transient expression and stable transformation.

Peptidase MA is one of the two zinc-dependent metallopeptidases, containing the HEXXH motif with the two His residues as zinc ligands. Up to now, over fifty metallopeptidase families have been identified. In these enzymes, a divalent cation, usually zinc but may be cobalt, manganese or copper, activates the water molecule through the nucleophilic attack on a peptide bond. Thus, metallopeptidases belong to hydrolases. Depending on the number of metal ions required for the catalysis, metallopeptidases can be divided into two classes: one class recruits only one metal ion for catalysis, while the other class, termed peptidase MA, needs to coordinate two metal ions. The upper-case letters in the name of a peptidase suggest the catalytic types: “M” represents for metallo-type, A for aspartic-type, S for serine-type, T for threonine-type, C for cysteine-type, and U for unknown type.

Figure 1. The structural model of endoplasmic reticulum aminopeptidase 2. (OPM Database)

With the Mempro™ plant-based protein production platform, Creative Biostructure can express, isolate, purify and crystallize peptidases MA to facilitate the study of their structural and biological functions. Additionally, post-translational modification such as glycosylation and disulfide bonds can be introduced in our plant-based expression system.

Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.
C. Trometer, and P. Falson (2010). Mammalian membrane protein expression in baculovirus-infected insect cells. Methods Mol. Biol., 601: 105-117.
F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.
Peptidase M10, metallopeptidase. InterPro. (http://www.ebi.ac.uk/interpro/entry/IPR001818)