HQExo™ Exosome-Pleural Fluid(Exo-BF07)
| Product Summary | |
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| Name | HQExo™ Exosome-Pleural Fluid |
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| Cat No. | Exo-BF07 |
| Source | Exosome derived from human Pleural Fluid (healthy donors) |
| Product Overview | Exosomes are small membrane vesicles with a size of 30-160 nm that are released by different cell types. They can be found in various body fluids including plasma, malignant ascites, urine, amniotic fluid and saliva, which contains a tissue-specific signature wherein a rich cargo of proteins, RNAs, cytokines and lipids are selectively packaged. Exosome could serve as an emerging platform for diagnostics. HQExo™ exosomes derived from body fluids could use as disease biomarkers research by ELISA, FACS, WB. Exosome are membranous structures and protected from degradation by extracellular proteases, which make the exosomes are highly stable. And due to the sample complexity reducing compared to the whole bodily fluids, exosome-based biomarker analysis has attracted more and more attention to clinical diagnostics. Exosome can be purified by ultracentrifugation and characterized by nanoparticles tracking analysis (NTA) and ELISA or WB. Lyophilization is useful for a long-term storage at 4°C, and frozen liquid should be kept at -20°C to -80°C. Creative Biostructure standard exosome products guarantee higher purity and quality to meet our customer’s downstream analyses. |
| Form | Lyophilized powder/ frozen liquid |
| Concentration | >1x10^6 particles |
| Storage | Lyophilized powder store at 4 °C. Frozen liquid store at -20°C to -80°C. Recommended to avoid repeated freeze-and-thaw cycles. |
| Reconstitution | Reconstitute lyophilized exosome by adding deionized water for a desired final concentration. Centrifuge before opening to ensure exosomes are at bottom, resuspend exosomes by pipetting and/or vortex, please avoid bubbles. Centrifuge again and mix well for using. |
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Frequently Asked Questions
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Do quiescent cells secrete exosomes?
Yes, quiescent cells are known to secrete exosomes. Exosomes are small vesicles that are released by various types of cells, including both quiescent (non-dividing) and actively dividing cells. These vesicles are involved in cell-to-cell communication, as they carry a cargo of proteins, lipids, and nucleic acids that can be transferred to recipient cells.
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What strategies can be employed to standardize mRNA expression within exosomes?
You can utilize miRNA genes like miRNA16, miRNA26a, miRNA221, miRNA22, miRNA181a, miRNA181c, miRNA103, miRNA191, let7d, as well as small RNAs (5SrRNA and U6snRNA) that are normally expressed in exosomes.
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Do you offer items designed for conducting studies on tracking extracellular vesicles?
Creative Biostructure offers highly purified fluorescent exosome standards for tracking EVs in fluorescence microscopy experiments. These fluorescent exosomes are labeled with a green dye, ensuring stable fluorescent labeling. They are suitable for various applications, providing a long-lasting and well-visible signal.
