HQExo™ Exosome-HCT116(Exo-CH22)
| Product Summary | |
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| Name | HQExo™ Exosome-HCT116 |
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| Cat No. | Exo-CH22 |
| Source | Exosome derived from human colorectal carcinoma cell line initiated from an adult male (HCT116 cell line) |
| Product Overview | Exosomes are nanosized vesicles (30-160 nm) secreted by exocytosis by most cell types and contain specifical cargos, such as RNAs, lipids, and proteins. The cargos amount and composition of exosomes depend on the cell type from which they are released, which making them useful for biomarker discovery and functional characterization. Exosomes have been isolated from cancer cell lines (human and mouse), which helps understand tumor growth microenvironments. Exosome derived from enormous model human cancer cell lines to improve the studies of tumor growth and invasion signaling pathways as well as how these tumor exosomes function and get an insight into antitumor research. HQExo™ standard exosomes could use as positive controls for exosome isolation and functional research, such as ELISA, FACS, WB. Lyophilization is useful for a long-term storage at 4°C, and frozen liquid should be kept at -20°C to -80°C. Ultracentrifugation and precipitation techniques are mainly used in exosome Isolation. It had been reported that both methods yielded extracellular vesicles in the size range of exosomes and included apoproteins, which can be used in downstream analyses. Nanoparticles Tracking Analysis (NTA) is used for measuring exosome particles concentration, and WB or ELISA can be used in exosomal biomarkers analysis. Creative Biostructure standard exosome products guarantee higher purity and quality to meet our customer research. |
| Form | Lyophilized powder/ frozen liquid |
| Concentration | > 1x10^8 particles |
| Storage | Lyophilized powder store at 4 °C. Frozen liquid store at -20°C to -80°C. Recommended to avoid repeated freeze-and-thaw cycles. |
| Reconstitution | Reconstitute lyophilized exosome by adding deionized water for a desired final concentration. Centrifuge before opening to ensure exosomes are at bottom, resuspend exosomes by pipetting and/or vortex, please avoid bubbles. Centrifuge again and mix well for using. |
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Frequently Asked Questions
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Do quiescent cells secrete exosomes?
Yes, quiescent cells are known to secrete exosomes. Exosomes are small vesicles that are released by various types of cells, including both quiescent (non-dividing) and actively dividing cells. These vesicles are involved in cell-to-cell communication, as they carry a cargo of proteins, lipids, and nucleic acids that can be transferred to recipient cells.
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What strategies can be employed to standardize mRNA expression within exosomes?
You can utilize miRNA genes like miRNA16, miRNA26a, miRNA221, miRNA22, miRNA181a, miRNA181c, miRNA103, miRNA191, let7d, as well as small RNAs (5SrRNA and U6snRNA) that are normally expressed in exosomes.
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Do you offer items designed for conducting studies on tracking extracellular vesicles?
Creative Biostructure offers highly purified fluorescent exosome standards for tracking EVs in fluorescence microscopy experiments. These fluorescent exosomes are labeled with a green dye, ensuring stable fluorescent labeling. They are suitable for various applications, providing a long-lasting and well-visible signal.
