Super-resolution Stimulated Emission Depletion Microscopy with Gated Detection (gSTED) Service
To probe cell biology at the molecular level and to visualize single molecule activity with macroscopic cellular behavior in real time, novel super-resolution far-field microscopy (or nanoscopy) approaches have been developed. Stimulated emission depletion microscopy with gated detection (gSTED) is one of the most advanced techniques that allow the acquisition of high-resolution images by point scanning over the sample, generating images pixel by pixel.
In gSTED, a diffraction-limited spot is excited at a certain wavelength; and a super-imposed, red-shifted, second laser beam projects to deplete a donut-shaped area, leaving only a central focal spot with a dimension below the diffraction limit. The size of the center focal spot can be tuned by changing the intensity of the depletion laser such that a lateral resolution of less than 50 nm could be achieved. As gSTED utilizes pulsed excitation in combination with continuous wave (CW) laser depletion and time-gated detection, an important prerequisite is to use CW lasers with low noise; because any fluctuations in the power level compromise the performance of this technique.
The superior resolution gSTED has several features over conventional confocal microscopy:
- Better stability and reliability
- Full spectrum of visible light and optimal color correction
- Improved resolution
- Deep live imaging and improved signal to noise ratios
Creative Biostructure provides gSTED services with the following advantages:
- Professional scientific team
- State-of-the-art technologies
- Highly reliable and reproducible result
- Competitive price in the market
Creative Biostructure promises to work closely with our customers to provide excellent services. Please feel free to contact us for more information or a detailed quote.
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References
- Wegel E, et al. Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison. Scientific Reports. 2016, 6(1): 1-13.
- Eggeling C, Honigmann A, Schulze M. gSTED Microscopy with an OPSL: Cutting Edge Super‐Resolution: Breaking the diffraction limit with a CW OPSL. Optik & Photonik. 2012, 7(2): 44-46.
- Vicidomini G, et al. STED nanoscopy with time-gated detection: theoretical and experimental aspects. PloS One. 2013, 8(1): e54421.
