Nuclear Magnetic Resonance (NMR) Platform

MagHelix™ Nuclear Magnetic Resonance Spectroscopy (NMR) platform provides clients a broad array of services ranging from the production of labeled proteins to the acquisition and analysis of high field NMR data. Wide ranges of samples are tested, including both liquid and solid-state samples. Creative Biostructure experts will assist you in designing and developing a suitable plan regarding your research of interest. We promise quality analysis with rapid turnaround times. Services can be offered to Good Laboratory Practice (GLP) or Good Manufacturing Practice (GMP) if needed.

Figure 1. Workflow for protein structure determination by NMR

Platform Equipment
Our MagHelix™ platform is characterized by its state-of-the-art NMR instrumentation, which includes Bruker AVANCE 900 MHz, AVANCE III 600 MHz-HD, AVANCE III-NanoBay and other spectrometers. With these modern NMR equipment, we can offer our specialized services to customers in academia and various industries.

Bruker AVANCE 900 MHz Spectrometer
Our 900 MHz digital NMR spectrometer equipped with TCI Cryo probe, VT CPMAS probe, and other probes, is one of the most powerful and sensitive NMR instrumentations. It enables performance of both sophisticated solution and solid-state NMR experiments.

Bruker AVANCE III-HD
This superconducting FT NMR Spectrometer features the most advanced RF generation in the field, and ideal for fast acquisition techniques of samples.

Bruker AVANCE III-NanoBay
Combine with CryoProbe Prodigy and autosampler SampleXpress, it represents the most highly integrated NMR spectrometer, and offers efficient structure elucidation of small molecules, therefore, is great for mixture identification and quantification.

NMR Software
A wide range of NMR software are installed in our NMR workstation. Topspin2.1，NMRPipe/TALOS, and NUTS for spectral processing; Sparky and Autoassign for assignments; Procheck for validation; PyMol and MolMol for molecular graphics.

Services
Efficient and reliable NMR structure determination requires acquisition of high quality experimental data, application of computational NMR methods and specialist knowledge for interpretation and manual fine-tuning of the results. With tremendous experience from our senior scientists, Creative Biostructure develops this NMR platform to support NMR services including but not limited to:

• Compound structure determination
• Chemical composition
• Quality control
• Quantitative analysis
• Sample purity determination
• Dynamics, disorder
• Molecular characterization
• Reaction kinetics examination

Creative Biostructure set high standards for our service and keep up with the latest developments in the science and technology. Our NMR platform support the clients with projects of structural determination and interaction of protein/peptide drugs with targets. Each project is tailored to the customer’s specifications of scale, purity, analytical characterization and activity. Please do not hesitate to contact us.

Ordering Process

Reference:

1. Wüthrich K (2003). “NMR studies of structure and function of biological macromolecules (Nobel lecture)”. J. Biomol. NMR, 27: 13-39
2. Cheng X (2013). “Structure and interaction of the human programmed cell death 1 receptor”. J.Biol.Chem., 228: 11771-11785