Microbubble Services

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Microbubbles are a new, economically viable, non-invasive alternative to traditional techniques. Microbubbles range in size from 1-100 μm and contain oxygen or air and remain in suspension to prolong drug release. Due to their great potential, microbubbles are used for the delivery of therapeutic drug molecules or genetic material to specific targets in various diseases.

Figure 1. Preparation of micro-bubbles by batch sonication technique. (Sandeep, R., et al., 2021)

Construction of gene-loaded multifunctional polymeric microbubbles

Microbubble preparation	Polymeric microbubble contrast agents were prepared by a single emulsification method. Cationic polymers were used to encapsulate plasmid DNA. Targeted modification of polymeric microbubbles by covalent coupling method.
Microbubble characterization	Optical microscopy to observe microbubble morphology and measure particle size. Qualitative detection of plasmid and antibody binding by fluorescence microscopy. Quantitative detection of plasmid and antibody binding by flow cytometry.
Targeted binding assay	Tumor cells are cultured in vitro. Add multifunctional microbubbles for target binding. Set up the antibody-blocking experimental group. Microscopic observation of multifunctional microbubbles and cell target binding.

Construction of ultrasonic microbubble-mediated gene delivery system

Co-hybrid delivery system. The microbubbles, genes, and cells are in close contact so that the microjets or vibrations generated by microbubble rupture act on the cell membrane surface, enabling rapid gene entry into cells.
Loaded delivery system. Prepared by physically wrapping the gene drug inside the microbubble or electrostatically adsorbing it to the microbubble surface. The further surface modification allows specific delivery of the microbubble to the target cell, achieving close contact between the microbubble, gene, and cell, and greatly improving the efficiency of gene expression.
Coupled delivery system. It has sufficient stability for long circulation in the blood to the tumor site and can be targeted by loading ultrasound at specific tissues to achieve local release of the gene carrier.

Technical advantages

By encapsulating protein drugs in microbubbles for drug delivery, the direct exposure of proteins to the body's immune system can be avoided, reducing the body's immune rejection response and achieving the desired therapeutic effect.
It increases the permeability of local microvasculature and cell membranes, facilitating the penetration of the drug, thus achieving localized drug release in vivo and improving the therapeutic effect.

Creative Biostructure can prepare microbubbles for your application with great potential. Please contact our staff to learn how we can provide you with solutions related to microbubble drug delivery systems.

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Reference

Rathor S, et al. A comprehensive review on microbubble concept, development and its application in therapeutic drug delivery and clinical management of disease. Current Pharmaceutical Biotechnology. 2021. 22(11): 1424-1443.