Gel Permeation Chromatography Analysis
To provide a comprehensive understanding of polymer materials, Creative Biostructure offers gel permeation chromatography analysis services. Our aim is to deliver precise and dependable molecular weight data that can significantly enhance the development and quality control processes in various industries.
What Is Gel Permeation Chromatography Analysis?
Gel permeation chromatography analysis, commonly known as GPC, is a type of chromatography technique used to separate polymers based on their molecular size. It is widely utilized in chemistry, biology, pharmacy, and materials science to determine the molecular weight and distribution of polymer materials.
Principle: GPC involves the separation of molecules based on their size as they pass through a column packed with porous gel. Larger molecules elute faster as they cannot penetrate deeply into the pores of the gel, while smaller molecules elute slower as they diffuse into the pores more extensively.
Stationary Phase: The column is filled with porous material that contains small pores with various sizes. The stationary phase is essentially inert and does not interact with the analytes chemically.
Mobile Phase: A solvent is used to carry the sample through the column. The choice of solvent depends on the nature of the polymers being analyzed.
Figure 1. General GPC result and GPC chromatogram result. (Creative Biostructure)
Why Perform Gel Permeation Chromatography Analysis?
- Understanding Polymer Properties: The molecular weight and its distribution are crucial in determining the properties and applications of polymer materials. GPC provides insights into these characteristics, which is essential for material development and application.
- Simple Operation: GPC is known for its ease of use. The process involves injecting a polymer solution into a column filled with porous gel, which simplifies the operation compared to other molecular weight determination methods.
- High Resolution and Repeatability: The technique offers high resolution, allowing for the separation of polymer molecules based on their molecular weight. Additionally, GPC is known for its good repeatability, ensuring consistent results in molecular weight measurements.
- Versatility in Applications: GPC is applicable in various fields such as research and development of new materials and quality control in the industrial sector. It plays an irreplaceable role in both scientific and industrial settings.
Our Gel Permeation Chromatography Analysis
Creative Biostructure's GPC analysis service is designed to be rigorous and efficient, ensuring a professional and convenient experience for our clients. Our streamlined service process includes the following steps:
1. Initial Consultation: We initiate the process with a thorough consultation where clients detail their testing requirements and provide sample information. Our dedicated customer service team promptly responds, offering expert advice and guidance tailored to their needs.
2. Sample Receipt and Registration: Upon receiving the samples, we meticulously document and register them to ensure full traceability. We also confirm the testing protocols and standards with the client, guaranteeing the precision and reliability of the outcomes.
3. Sample Pre-treatment: Our technical team conducts meticulous pre-treatment of the samples, including dissolution, dilution, and filtration, as required. This step is crucial for eliminating any impurities or interferences, thereby ensuring the accuracy of the subsequent analysis.
4. GPC Analysis: Leveraging state-of-the-art GPC equipment, we perform the testing with high-resolution and sensitivity. Adhering strictly to international standards and client-specific requirements, we ensure that the molecular weight and distribution determinations are both precise and dependable.
5. Data Interpretation and Reporting: Post-testing, our team of analysts meticulously interprets the data and compiles a comprehensive report. This document outlines the molecular weight and distribution of the samples, accompanied by detailed analysis and insights. We present the report in either electronic or hard copy, as per the client's preference, and provide additional consultation services for a deeper understanding of the results.
Figure 2. The workflow of our gel permeation chromatography analysis. (Creative Biostructure)
At Creative Biostructure, we are committed to delivering a service that not only meets but exceeds expectations, ensuring that our clients receive the highest quality GPC testing services in the industry. If you are interested, please contact us for a detailed quote.
Key Advantages of Our of GPC Analysis Services
- Rigorous Process: From consultation to sample receipt and registration, our stringent procedures ensure accurate and reliable results.
- Expert Pre-treatment: Customized pre-treatment methods such as dissolution, dilution, and filtration to eliminate impurities and enhance accuracy.
- Advanced Equipment: High-resolution and sensitive GPC technology, following strict international and customer-specific standards for precise molecular weight determination.
- Comprehensive Analysis: Detailed data analysis with thorough result interpretation provided in customizable report formats (electronic or paper) with added consultation services.
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Gel Permeation Chromatography Analysis
Frequently Asked Questions
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Why must samples have good solubility in the specified mobile phase for GPC testing?
GPC is a chromatographic test that separates components of different molecular weights through a chromatography column. If the sample has poor solubility, it must be filtered before testing. Components that do not dissolve cannot be tested for their molecular weight, and if the sample has poor solubility, it may precipitate in the chromatography column, damaging the column.
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What is the difference between relative and absolute molecular weights?
Relative Molecular Weight refers to the molecular weight of a substance in comparison to the molecular weight of another substance. Absolute Molecular Weight, on the other hand, is the actual molecular weight of a substance, expressed as an absolute value without comparison to other substances.
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What should I do if I have questions about the test report?
If you have any questions or require further explanation regarding the test report, please feel free to contact our customer service team at any time. Our team will provide you with professional answers and support to ensure you fully understand the content and significance of the test report.
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