In this study, researchers used the single LCNAN particle freezing electron microscopy to explore the mechanism. Since the quaternary complex of NALCN-FAM155-UNC79-UNC80 is not stable enough, they focus on the structural analysis of the relatively stable core subunit of NALCN-FAM155. After homologous protein screening and other steps, they determined the complex composed of rat NALCN and mouse FAM155a subunit as the research object. After overcoming the difficulties of sample preparation…