Mempro™ Cell-Free DNase I-like Protein Production

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Based on the comprehensive and excellent membrane protein production platform established through years of experience, scientists from Creative Biostructure can provide first-class custom DNase I-like protein production services through our best Mempro™ cell-free protein expression system for global customers.

Mempro™ cell-free protein production system is the renewed platform developed by Creative Biostructure, which can bypass the limitations that encountered in cell-based protein production system, such as host cell toxicity, low yield and solubilization and purification using detergents, etc.

DNase I-like protein belongs to a protein superfamily that is composed of three families, including endonuclease/exonuclease/phosphatase (EEP) family, arthropod phospholipase D and bacterial phosphatidylinositol-specific phospholipase C. The EEP family can be widely found in endonucleases and phosphatases involved in intracellular signaling. Members of arthropod phospholipase D are actually phospholipases and sphingomyelinases that are identified in a wide range of organisms, such as bacteria, yeast, plants, animals, and viruses. The bacterial phosphatidylinositol-specific phospholipase C are small, water-soluble and calcium-independent enzymes that comprise a single domain folded as a (beta alpha) (8)-barrel. In addition, inositol polyphosphate 5-phosphatase (IPP5) encoded by ITPA gene in humans is one of the members of DNase I-like protein superfamily.

Mempro™ Cell-Free DNase I-like Protein Production Figure 1. Structural model of Type II inositol-1,4,5-trisphosphate 5-phosphatase. (OPM Databse)

Creative Biostructure can perform various strategies for high-quality Mempro™ cell-free DNase I-like proteins, including:

• Mempro™ DNase I-like proteins Production in Bacterial Extract
E. coli S30 extract is commonly applied for membrane protein production. Mempro™ membrane protein expression by bacterial extract provides customers the enhancement of expression of the DNase I-like proteins, and easy to perform scale-up membrane protein production.

• Mempro™ DNase I-like proteins Production in Wheat Germ
Creative Biostructure provides eukaryotic translation system for DNase I-like proteins production in wheat germ system. This system facilitates the membrane proteins performing correct folding and post-translational modifications and maintaining the correct structural and functional properties.

• Mempro™ DNase I-like proteins Production in Rabbit Reticulocyte
Crude rabbit reticulocyte lysate is the innovative system for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.

• Mempro™ DNase I-like proteins Production in Baculovirus
Baculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also an important host for DNase I-like proteins expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.

Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
C. Q. Pan, et al. (1998). Mutational analysis of human DNase I at the DNA binding interface: implications for DNA recognition, catalysis, and metal ion dependence. Protein science, 7(3): 628-636.
F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380.